Effect of whole body vibration on bone nanocomposites organisation and prevention of loss of bone mineral density under conditions of modeling obesity and sedentary lifestyle: experimental study





whole body vibration, bone remodeling, bone mineral density, osteoporosis, X-ray diffraction


This study aimed to investigate the influence of high-frequency whole body vibration (WBV) on metabolic and structural responses of rats' bone tissue under the sedentary lifestyle and obesity. Obesity combined with a sedentary lifestyle can present the potential negative health effects. However, whole body vibration can be used as a means of non-pharmacological correction of bone mineral density. For characterization of bone nanocomposites organisation and prevention of mineral density loss, X-ray diffraction method was used. Markers of bone remodeling in the rats' blood: leptin, osteocalcin, tartarate resistant acid phosphatase 5b, alkaline phosphatase. Using a high-calorie diet and low-mobility model, we proved that bone mineral mass had been decreasing since 8th week. It should be noted that the decrease in the relative amount of crystalline phase (hydroxyapatite) continued throughout the experiment, up to 24 weeks (p<0.05). These structural changes were accompanied by changes in quantitative indicators of the bone remodeling markers. Rats had lower bone mineral density compared to the animals that were on the normal diet and were additionaly affected by WBV. We observed the increase of the crystalline phase volume fraction from 84% to 93% (p<0.05) in group with additional whole body vibration and the decrease of the mineral component in rats with limited mobility and high-calorie diet. Therefore, WBV could improve structural conditions of bone and prevent fat accumulation and obesity-associated biochemical markers in obese rats. This can be an effective method to improve the structural and functional state of the bones while preventing the loss of bone mineral density.


Antamonov MY. [Mathematical processing and analysis of medical and biological data. Kyiv; 2017.

Alavinia SM, Omidvar M, Craven BC. Does whole body vibration therapy assist in reducing fat mass or treating obesity in healthy overweight and obese adults? A systematic review and meta-analyses. Disability and Rehabilitation. 2019;1-13. doi: https://doi.org/10.1080/09638288.2019.1688871

Bunaciu AA, UdriŞTioiu EG, Aboul-Enein HY. X-ray diffraction: instrumentation and applications. Cri¬tical reviews in analytical chemistry. 2015;45(4):289-99. doi: https://doi.org/10.1080/10408347.2014.949616

Clark SM, Iball J. The x-ray crystal analysis of bone. Progress in Biophysics and Biophysical Chemistry: Progress Series. 2016;7:226. doi: https://doi.org/10.1016/S0096-4174(18)30127-6

Morris HA, Eastell R, Jorgensen NR, Cavalier E, Vasikaran S, Chubb SAP, et al. Clinical usefulness of bone turnover marker concentrations in osteoporosis. Clinica chimica acta. 2017;467:34-41. doi: https://doi.org/10.1016/j.cca.2016.06.036

Londoño-Restrepo SM, Jeronimo-Cruz R, Millán-Malo BM, Rivera-Muñoz EM, Rodriguez-García ME. Effect of the nano crystal size on the X-ray diffraction patterns of biogenic hydroxyapatite from human, bovine, and porcine bones. Scientific reports. 2019;9(1):1-12. doi: https://doi.org/10.1038/s41598-019-42269-9

Gritschmeier F, Lange KW. Health effects of whole body vibration. Movement and Nutrition in Health and Disease. 2020;4:83-88.

Hlaing TT, Compston JE. Biochemical markers of bone turnover–uses and limitations. Annals of clinical biochemistry. 2014;51(2):189-202. doi: https://doi.org/10.1177/0004563213515190

Minematsu A, Nishii Y, Imagita H, Sakata S. Whole body vibration at low-frequency can increase trabecular thickness and width in adult rats. Journal of musculoskeletal & neuronal interactions. 2019;19(2):169.

Pang MY, Lau RW, Yip SP. The effects of whole-body vibration therapy on bone turnover, muscle strength, motor function, and spasticity in chronic stroke: a randomized controlled trial. European journal of physical and rehabilitation medicine. 2013;49(4):439-50.

Savvidis C, Tournis S, Dede AD. Obesity and bone metabolism. Hormones. 2018;17(2):205-17. doi: https://doi.org/10.1007/s42000-018-0018-4

Shapses SA, Pop LC, Wang Y. Obesity is a concern for bone health with aging. Nutrition research. 2017; 39:1-13. doi: https://doi.org/10.1016/j.nutres.2016.12.010

Smith KB, Smith MS. Obesity statistics. Primary care: clinics in office practice. 2016;43(1):121-35. doi: https://doi.org/10.1016/j.pop.2015.10.001

Li W, Xu P, Wang C, Ha X, Gu Y, Wang Y, Xie J. The effects of fat-induced obesity on bone metabolism in rats. Obesity Research & Clinical Practice. 2017;11(4):454-63. doi: https://doi.org/10.1016/j.orcp.2016.12.001

McGee-Lawrence ME, Wenger KH, Misra S, Davis CL, Pollock NK, Elsalanty M, et al. Whole-body vibration mimics the metabolic effects of exercise in male leptin receptor–deficient mice. Endocrinology. 2017;158(5):1160-71. doi: https://doi.org/10.1210/en.2016-1250

Zago M, Capodaglio P, Ferrario C, Tarabini M, Galli M. Whole-body vibration training in obese subjects: A systematic review. PloS one. 2018;13(9):e0202866. doi: https://doi.org/10.1371/journal.pone.0202866

Flores J, Jimenez A, Alonso A, Cervantes N, Hernandez N, et al. Whole body vibration training with repetitive interval musculoskeletal loading on bone in young women. Southern California Conferences for Undergraduate Research; 2017.




How to Cite

Kostyshyn N, Gzhegotskyi M, Yarova O, Kostyshyn L, Kulyk Y. Effect of whole body vibration on bone nanocomposites organisation and prevention of loss of bone mineral density under conditions of modeling obesity and sedentary lifestyle: experimental study. Med. perspekt. [Internet]. 2021Mar.26 [cited 2023Nov.30];26(1):30-9. Available from: https://journals.uran.ua/index.php/2307-0404/article/view/227726