The effect of stress on the hematological indicators of rats Rattus Norvegicus F. Domesticus in the conditions of the biological experiment
Keywords:laboratory rats, stress, morphological indicators, hematological indicators
The purpose of the work was the need to study the change in weight and hematological indicators of the rats as a reaction-response to being in difficult and unfavorable experimental conditions. The biological experiment was conducted in compliance with the normative conditions of keeping the rats, in accordance with ethical standards and recommendations for humanization of work with laboratory animals. In order to accomplish these tasks, rats were divided into two groups: control rats were housed in a spacious cage with comfortable living conditions and experimental animals were housed in a small cage with limited ability to move freely. For the purity of the experiment, the feed ration of animals of two groups was the same. Weight and hematological indicators were determined by conventional methods in physiology. It has been found that improper housing conditions, causing a stressful situation adversely affect the eating activity of the test animals, which explains the weak correlation of the dynamics of the weight of the animals in the control and experimental groups. The analysis of the absolute mass of the internal organs of rats showed their direct dependence on body weight and the correlation of these parameters between animals of both groups. The study found that the quantitative ratios of the main hematological parameters of each of the rodents are strictly different. The tendency to increase of the investigated parameters in the experimental group in comparison with the control one is symptomatic and can be explained by sympathetic-vegetative influences. The amount of hemoglobin is directly correlated with the number of red blood cells. Comparison of the content of hemoglobin, erythrocytes, leukocytes with body weight showed inversely proportional relationship between them. The persistent predominance of leukocytes in the blood of rats in both groups is likely to genetically determined.
Antomonov MYu. [Mathematical processing and analysis of medico-biological data]. 2nd ed. Kyi: MIC "Medinform"; 2018. p. 579. Russian.
[Healthy eating: the calorie table of products]. Medfund. 2018;1. Ukrainian. Availabe from: https://medfond.com/static/tablicya-kaloriinosti-produktiv.html.
Kotsiumbas G, Tesarivska U, Humenetska M, Shumsk M. [Hematological parameters and morphological characteristics of the spleenin femalerats f1 underin fluense nanohermanium citrate, used indifferent doses]. Scientific Messenger Stepan Gzhytskyi NUVMBL. 2017;19:77:45-50. Ukrainian. doi: https://doi.org/10.15421/nvlvet7711
Litovchenko OL, Pertsev DP, Zavgorodnii IV, Mitelova TU, Chehovska IM, Abramova LP, Vekshin VO. [Biochemical mechanisms of mixed effect of electromagnetic radiation and low positive temperature on animals’ organism]. Annals of Mechnikov Institute. 2015;2:119. Ukrainian. Availabe from: http://www.imiamn.org.ua/journal/2_2015/PDF/21.pdf
[Scientific and practical recommendations for keeping and working with laboratory animals: manual]. Kozhemyakin YuM, Khromov OS, Boldyreva NE, Dob¬relya V, Saifetdinova GA, editors. Kyiv: Interservice; 2017;182. Ukrainian. ISBN 978-617-696-560-2.
[Order 01.03.2012 No. 249, dated 16th March, 2012 No. 416/20729 on approval of carrying out experiments on animals by scientific establishments]. 2012. Ukrainian.
Sorokman TV, Tkach VV. [Factors influencing the development of eating disorders. Prospects for the development of medical science and education: a collection of abstracts of the All-Ukrainian scientific and methodological conference, dedicated to the 25th anniversary of the Sumy State University Medical Institute]. Sumy: Sumy State University; 2017. p. 108. Ukrainian. Availabe from: https://essuir.sumdu.edu.ua/bitstream/123456789/64734/1/Sorokman_faktory.pdf
[Higher education standard of the second (mas¬ter's) level, field of knowledge 22 Health care, specialty 221 Dentistry. Approved and implemented by the order of the Ministry of Education and Science of Ukraine No. 879 dated 24.06.2019]. 2019. Ukrainian. Availabe from: https://mon.gov.ua/storage/app/media/vishcha-osvita/zatverdzeni%20standarty/2019/06/25/221-stomatologiya-magistr.pdf.
Toziuk OYu, Kryvoviaz OV, Ivko TI, Voronkina AS. [Pharmacological effects of KB-28 compound under chronic immobilization stress conditions]. World of medicine and biology. 2018;1(63):160-3. Ukrainian. doi: https://doi.org/10.26724/2079-8334-2018-1-63-160-163
Barkasi Z. [Muroid Rodents of the Lowland Part of Transcarpathia: State of Populations and Morphophysiology]. Proceedings of the Theriological School. 2015; 13(13):3-10. Ukrainian. doi: https://doi.org/10.15407/ptt2015.13.003
Ju Hwan Kim, Jin-Koo Lee, Hyung-Gun Kim, Kyu-Bong Kim, and Hak Rim Kim. Possible effects of radiofrequency electromagnetic field exposure on central nerve system. Biomol Ther. 2019;27(3):265-75. doi: https://doi.org/10.4062/biomolther.2018.152
Makowska IJ, Weary DM. Differences in Anti¬cipatory Behaviour between Rats (Rattus norvegicus) Housed in Standard versus Semi-Naturalistic Labo¬ratory Environments Plos one. 2016;28:1-2. doi: https://doi.org/10.1371/journal.pone.0147595
Wilson Jacob Filho, Caio Cezar Lima, Marcos Rodolfo Ramos Paunksnis, Ariana Aline Silva, Mauro Sérgio Perilhão, Marina Caldeira, et al. Reference database of hematological parameters for growing and aging rats. The Aging Male. 2018;21(2):145-148. doi: https://doi.org/10.1080/13685538.2017.1350156
Shuren J, Patel B. FDA regulation of mobile medical apps. JAMA. 2018 Jul 24;320(4):337-8. doi: https://doi.org/10.1001/jama.2018.8832
The Laboratory Rat (Third Edition) A volume in American College of Laboratory Animal Medicine. Academic Press, 2019;215-42. doi: https://doi.org/10.1016/C2017-0-01188-6
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