Morphology of the rat knee joint after intraarticular injection of hydroxymethyl-quinoxaline dioxide

Authors

DOI:

https://doi.org/10.26641/2307-0404.2022.3.265824

Keywords:

hydroxymethyl-quinoxaline dioxide, experiment, rats, knee joint, histology

Abstract

Topical antiseptics are widely used in orthopedics and traumatology. However, studies highlighting the effects of antiseptics during long-term use on joint tissues are limited. In the performed work, the peculiarities of the structure of intra-articular tissues of the knee joint and muscles in rats in two series of experiments were noted: control group (saline injection into the joint) and experimental group (hydroxymethyl-quinoxaline dioxide injection into the joint) daily for 5 days. Histological methods with semi-quantitative assessment of articular cartilage and synovial membrane were used. During the first day after the drug administration, similar reactive changes to the injection of saline and hydroxymethyl-quinoxaline dioxide were observed in the joint tissues of the two groups of rats. The synovial membrane of the capsule was thickened, and synoviocytes were hypertrophied. The articular cartilage contains single cells with dense nuclei only in the superficial parts, but in general, the cytoarchitectonics of articular cartilage was preserved. In 5 days after injection, no statistically significant differences between saline and hydroxymethyl-quinoxaline dioxide were observed for the study periods. According to the studies the safety of long-term use of the drug as an antiseptic for articular tissues was confirmed.

References

Fuchs M, Pumberger M, Hommel H, Perka C, von Roth P, Thiele K. Bacterial Colonization of Irrigation Fluid during Aseptic Revision Knee Arthroplasty. J Clin Med. 2020;9(9):2746. doi: https://doi.org/10.3390/jcm9092746

Loskutov AE. Dehtyar AV, Ctepanskyi DA. [Comparative evaluation of the antimicrobial activity of local antiseptics in revision hip arthroplasty]. Medicni perspektivi. 2017;22(1):25-32. Ukrainian. doi: https://doi.org/10.26641/2307-0404.2017.1.100870

Shahi A, Parvizi J. Prevention of Periprosthetic Joint Infection. Arch Bone Jt Surg. 2015;3(2):72-81. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4468618/

Calkins TE, Culvern C, Nam D, Gerlinger TL, Levine BR, Sporer SM et al. Dilute betadine lavage reduces the risk of acute postoperative periprosthetic joint infection in aseptic revision total knee and hip arthroplasty: a randomized controlled trial. J Arthroplasty. 2019;35(2):538-43. doi: https://doi.org/10.1016/j.arth.2019.09.011

Goswami K, Austin MS. Intraoperative povidone-iodine irrigation for infection prevention. Arthroplast Today. 2019;5:306-8. doi: https://doi.org/10.1016/j.artd.2019.04.004

Koburger T, Hubner NO, Braun M, Siebert J, Koburger T. Standardized comparison of antiseptic efficacy of triclosan, PVP-iodine, octenidine dihydrochloride, poly-hexanide and chlorhexidine digluconate. Antimicrob Chemother. 2010;5(8):1712-9. doi: https://doi.org/10.1093/jac/dkq212

Wood T, Ekhtiari S, Mundi R, Citak M, Sancheti PK, Guerra-Farfan E, et al. The Effect of Irrigation Fluid on Periprosthetic Joint Infection in Total Hip and Knee Arthroplasty: A Systematic Review and Meta-Analysis. Cureus. 2020;12(4):e7813. doi: https://doi.org/10.7759/cureus.7813

Akhtyamov IF, Rimashevskii DV, Kurmanga-liev Е-DT, Moldakulov ZhM, Eremin IK. [Prevention of infectious complications of knee arthroplasty]. Meditsin-skaia praktika. Kazan, 2014. Russian.

Hart A, Hernandez NM, Abdel MP, Mabry TM, Hanssen AD, Perry KI. Povidone-iodine wound lavage to prevent infection after revision total hip and knee arthroplasty. J Bone Joint Surg. 2019;101(13):1151-59. doi: https://doi.org/10.2106/JBJS.18.01152

European Convention for the protection of vertebrate animals used for experimental and other scientific purposes. Strasburg; 1986(123). Available from: https://rm.coe. int/168007a67b

[Order of the Ministry of Education and Science of Ukraine No. 249 of March 1, 2012 “Procedure for conduc¬ting experiments and experiments on animals by scientific institutions]. (2012). Available from: https://zakononline.com.ua/documents/show/218506___218571

Sarkisov DS, Perov YuL, editors. [Microscopic technique. A guide for physicians and laboratory assis-tants]. Moskva: Meditsina; 1996. Russian.

Gerwin N, Bendele AM, Glasson S, Carlson CS. The OARSI histopathology initiative – recommendations for histological assessments of osteoarthritis in the rat. 2010;18(Suppl. 3):S24-34. doi: https://doi.org/10.1016/j.joca.2010.05.030

Grzhibovsky AM, Ivanov SV, Gorbatova MA. [Comparison of Quantitative Data from Two Independent Samples Using Statistica and SPSS Software: Parametric and Nonparametric Tests]. Nauka i zdravoohranenie. 2016;2:5-28. Russian. Available from: https://cyberleninka.ru/article/n/sravnenie-kolichestvennyh-dannyh-dvuh-nezavisimyh-vyborok-s-ispolzovaniem-programmnogo-obespecheniya-statistica-i-spss.

Boddapati V, Fu MC, Mayman DJ, Su EP, Sculco PK, McLawhorn AS. Revision total knee arthroplasty for periprosthetic joint infection is associated with increased postoperative morbidity and mortality relative to noninfectious revisions. J Arthroplast. 2018;33:521-6. doi: https://doi.org/10.1016/j.arth.2017.09.021

`Wood T, Ekhtiari S, Mundi R, Citak M, Sancheti PK, Guerra-Farfan E, et al. The Effect of Irrigation Fluid on Periprosthetic Joint Infection in Total Hip and Knee Arthroplasty: A Systematic Review and Meta-Analysis. Cureus. 2020;12(4):e7813. doi: https://doi.org/10.7759/cureus.7813

Frisch NB, Kadri OM, Tenbrunsel T, Abdul-Hak A, Qatu M, Davis JJ. Intraoperative chlorhexidine irrigation to prevent infection in total hip and knee arthroplasty. Arthroplast Today. 2017;12:294-97. doi: https://doi.org/10.1016/j.artd.2017.03.005

Dehtyar VA, Dehtyar AV. Dedukh NV, Nikolchenko OA. [Short and long-term effects of the antiseptic decametoxin (dekasan®) on structural components of the knee joint in conditions intra-articular introduction (experimental study)]. Visnyk problem biolohii i medy-tsyny. 2019;1-2(149):286-90. Ukrainian. doi: https://doi.org/10.29254/2077-4214-2019-1-2-149-286-290

Downloads

Published

2022-09-30

How to Cite

1.
 Degtiar A. Morphology of the rat knee joint after intraarticular injection of hydroxymethyl-quinoxaline dioxide. Med. perspekt. [Internet]. 2022Sep.30 [cited 2024Nov.5];27(3):33-8. Available from: https://journals.uran.ua/index.php/2307-0404/article/view/265824

Issue

Section

THEORETICAL MEDICINE