Brain aquaporin-4 expression in the rat septic model (immunohistochemical study)
DOI:
https://doi.org/10.26641/2307-0404.2022.3.265827Keywords:
sepsis-associated encephalopathy, astroglial reactivity, aquaporin-4Abstract
The study aimed to determine aquaporin-4 expression in different brain regions was performed in Wistar rats subjected to cecal ligation and puncture (CLP) septic model. The immunohistochemical study of aquaporin-4 was carried out in the sensorimotor cortex, white matter, hippocampus, thalamus and caudate nucleus/putamen regions between 20 and 38 h after CLP. From the 12th h after CLP all animals showed the progressive impairment of sepsis signs and therefore, 9 rats were euthanized between 20-38 h (“CLP-B”, non-survived); 11 animals survived up to 48 h (constituted “CLP-A”, survived). After operation, CLP-B group displayed regionally-specific dynamic increase in aquaporin-4 level in the brain mostly associated with astroglial capillary endfeet: by 23rd h in the cortex – 234.15%, by 24th h in the thalamus –129.47% and hippocampus – 101.36%, by 30th h in the white matter – 135.31% and by 38 h in the caudate/putamen – 92.85%; with the highest increase in cortex: by 3.34 times. Heterogeneous and heterochronous aquaporin-4 elevation among brain regions indicates territories more and less susceptible for systemic toxic exposure in sepsis as well as points to diverse reactive responsiveness of local astroglial populations during specific time-period after CLP. The higher rates of aquaporin-4 in the cortex of non-survived animals in CLP model reflects the importance of aquaporin-4 increase in the mechanisms of sepsis decompensation.
References
Mazeraud A, Righy C, Bouchereau E, Benghanem S, Bozza FA, Sharshar T. Septic-Associated Ence-phalopathy: a Comprehensive Review. Neurotherapeutics. 2020 Apr;17(2):392-403. doi: https://doi.org/10.1007/s13311-020-00862-1
Chung HY, Wickel J, Brunkhorst FM, Geis C. Sepsis-Associated Encephalopathy: From Delirium to Dementia? J Clin Med. 2020 Mar 5;9(3):703. doi: https://doi.org/10.3390/jcm9030703
Rump K, Adamzik M. Function of aquaporins in sepsis: a systematic review. Cell Biosci. 2018 Feb 9;8:10. doi: https://doi.org/10.1186/s13578-018-0211-9
Ramadhanti J, Yamada T, Yasui M, Nuriya M. Differentially regulated pools of aquaporin-4 (AQP4) proteins in the cerebral cortex revealed by biochemical fractionation analyses. J Pharmacol Sci. 2021 May;146(1):58-64. doi: https://doi.org/10.1016/j.jphs.2021.03.003
Vandebroek A, Yasui M. Regulation of AQP4 in the Central Nervous System. Int J Mol Sci. 2020 Feb 26;21(5):1603. doi: https://doi.org/10.3390/ijms21051603
Tradtrantip L, Jin BJ, Yao X, Anderson MO, Verkman AS. Aquaporin-Targeted Therapeutics: State-of-the-Field. Adv Exp Med Biol. 2017;969:239-50. doi: https://doi.org/10.1007/978-94-024-1057-0_16
Verkman AS, Smith AJ, Phuan PW, Tradtrantip L, Anderson MO. The aquaporin-4 water channel as a potential drug target in neurological disorders. Expert Opin Ther Targets. 2017 Dec;21(12):1161-70. doi: https://doi.org/10.1080/14728222.2017.1398236
Gong W, Wen H. Sepsis Induced by Cecal Ligation and Puncture. Methods Mol Biol. 2019;1960:249-55. doi: https://doi.org/10.1007/978-1-4939-9167-9_22
Shulyatnikova T, Shavrin V. Mobilisation and redistribution of multivesicular bodies to the endfeet of reactive astrocytes in acute endogenous toxic encepha-lopathies. Brain Res. 2021 Jan 15;1751:147174. doi: https://doi.org/10.1016/j.brainres.2020.147174
Kim SW, Roh J, Park CS. Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips. J Pathol Transl Med. 2016 Nov;50(6):411-8. doi: https://doi.org/10.4132/jptm.2016.08.08
Antomonov MYu. [Mathematical processing and analysis of medical and biological data. 2nd ed.]. Кyiv: MYCz "Medynform"; 2018. р. 579. Russian.
Sfera A, Price AI, Gradini R, Cummings M, Osorio C. Proteomic and epigenomic markers of sepsis-indu-ced delirium (SID). Front Mol Biosci. 2015 Oct 26;2:59. doi: https://doi.org/10.3389/fmolb.2015.00059
Filippidis AS, Carozza RB, Rekate HL. Aquaporins in Brain Edema and Neuropathological Conditions. Int J Mol Sci. 2016 Dec 28;18(1):55. doi: https://doi.org/10.3390/ijms18010055
Meli R, Pirozzi C, Pelagalli A. New Perspectives on the Potential Role of Aquaporins (AQPs) in the Physiology of Inflammation. Front Physiol. 2018 Feb 16;9:101. doi: https://doi.org/10.3389/fphys.2018.00101
Ikeshima-Kataoka H. Neuroimmunological Implications of AQP4 in Astrocytes. Int J Mol Sci. 2016 Aug 10;17(8):1306. doi: https://doi.org/10.3390/ijms17081306
Shulyatnikova T, Tumanskiy V. Glutamine synthetase expression in the brain during experimental acute liver failure (immunohistochemical study). J Educ Health Sport. 2021 Oct;11(10):342-56. doi: https://doi.org/10.12775/jehs.2021.11.10.033
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