Evoked bioelectrical activity of efferent fibers of the sciatic nerve of white rats in experimental menopause.


  • A. G. Rodinsky
  • S. S. Tkachenko
  • L. V. Guz




experimental menopause, electrophysiological studies, ventral roots


The aim of our work was analysis of the bioelectrical activity of efferent fibers of the sciatic nerve in experimental menopause condition. Experiments were performed on 25 female white rats, divided into experimental and control groups. Menopause was modeled by total ovariohysterectomy. In 120 days after modeling we had recorded evoked action potentials of fibers of isolated ventral root L5 induced by stimulation of sciatic nerve with rectangular pulses. Threshold, chronaxia, latency, amplitude and duration of the action potential (AP) were analysed. Refractory phenomenon was investigated by applying paired stimuli at intervals of 2 to 20 ms. In the context of long-term hypoestrogenemy threshold of AP appearance was 55,32±7,69%, chronaxy – 115,09±2,67%, latent period – 112,62±1,74% as compared with the control animals (p<0.01). In conditions of paired stimuli applying the amplitude of response to the testing stimulus in animals with ovariohysterectomy at intervals 3 and 4 ms was 61,25±36,45% and 53,48±18,64% (p<0.05) respectively.


Makii EA, Nerush PA, Rodinskii AG, Mya-koushko VA. [Evoked Activity of Afferent and Efferent Fibers of the Sciatic Nerve in Rats under Conditions of Experimental Hyperthyroidism]. Neurophysiology. 2002;34(1):51-59. Ukrainian. 2. Malichenko SB, Volkova VA, Khalidova KK. [System changes in menopause. Role of deficiency of calcium and vitamin D in the formation of post-menopausal symptom]. Sovremennaya rheumatologiya. 2008;1:20-31. Russian. 3. Rodins'kiy OG, Tkachenko SS, Mozgunov OV. [Ele¬ctrophysiological analysis of neuromuscular complex excitability in experimental menopause]. Eksperym ta klin fiziol and biohim. 2014;(3):7-13. Ukrainian. 4. Fedotov YO, Sapronov NS. [Effects of estrogen in the central nervous system]. Uspekhi fiziologicheskikh nauk. 2007;38(2):46-52. Russian. 5. Unal D, Halici Z, Altunkaynak Z, Keles ON, Oral E, Unal B. A new hypothesis about neuronal degeneration appeared after a rat model of menopause. Neurodegener Diseases. 2012;9(1):25-30. 6. Bruce-Keller AJ, Keeling JL, Keller JN et al. An-tiinflammatory effects of estrogen on microglia activa-tion. J Endocrinol. 2000;141:3646-456. 7. Baulieu EE, Schumacher M. Progesterone as a neu¬roactive neurosteroid, with special reference to the effect of progesterone on myelination. Hum Reprod. 2000;15(1):1-13. 8. Koszykowska M, Wojtkiewicz J, Majewski M, Jana B. Effect of steroid hormones on the peripheral ner-vous system. Journal of Animal and Feed Sciences. 2008;17:3-18. 9. Frankenhaeuser B, Hodgkin AL. The action of calcium on the electrical properties of squid axons. J Physiol. 1957 July 11;137(2):218-44. 10. Fulop T, Agarwal, M, Agrahankar M, Arnold JL, Blumstein HA, Chrousos GP, et al. Hypomagnesemia. Medscape Reference (formerly eMedicine) from WebMD. Updated May 16, 2013. Available at: http://emedicine.medscape.com/article/2038394-overview. 11. Sreekantha, Satisha TG, Avinash SS, Manjunatha Goud BK, Remya, Sudhakar GK, et al. Magnesium and Calcium levels in early surgical menopause. J Clin Diagn Res. 2011;(1):55-57. 12. Mburu D. Menopause muscle pain and muscle tension: symptoms, causes, treatments. 2014 May 3. Last Modified: October 21, 2014. Available at: https://www.consumerhealthdigest.com/menopause-center/menopause-muscle-pain-and-tension.html 13. Li Y, Yang J, et al. N-myc downstream-regulated gene 2, a novel estrogen-targeted gene, is involved in the regu¬lation of Na+/K+-ATPase. J Biol Chem. 2011;286:32289-99. 14. O'Loughlin PD, Morris HA. Oestrogen deficiency impairs intestinal calcium absorption in the rat. J Physiol. 1998;511(1):313-22. 15. Ogunshola OO, Antic A, et al. Paracrine and autocrine functions of neuronal vascular endothelial growth factor (VEGF) in the central nervous system. J Biol Chem. 2002;277(13):11410-5. 16. Paul MR, Andrew JL, Benjamin KN, Thant SZ, Hedward C, Michael G. Steroid hormone signaling between schwann cells and neurons regulates the rate of myelin synthesis. Ann N Y Acad Sci. 2003;1007:340-8. 17. Verthelyi D. Female’s heightened immune status: estrogen, T cells, and inducible nitric oxide synthase in the balance. J Endocrinol. 2006;147(2):659-61.




How to Cite

Rodinsky AG, Tkachenko SS, Guz LV. Evoked bioelectrical activity of efferent fibers of the sciatic nerve of white rats in experimental menopause. Med. perspekt. [Internet]. 2016Mar.9 [cited 2024May29];21(1):4-9. Available from: https://journals.uran.ua/index.php/2307-0404/article/view/63440