Features of expression of oncoprotein p16ІNK4a in squamous cell carcinomas of the oropharynx.


  • O. V. Poslavskaya




squamous cell carcinoma of the oropharynx, HPV, prognostic and predictive markers, p16INK4a


Up to 40% of squamous cell carcinoma of the oropharynx (SCCO) are associated with human papillomavirus of a high oncogenic risk, which shows the greatest affinity to the epithelium of the tonsils. One of the indicators of malignant transformation of the multilayered epithelium cells with evidence of HPV lesions is the accumulation of oncoprotein p16INK4a, tumor suppressor  from Ink4 family. Presence of excessive amount of p16INK4a in a free state shows its reduced suppressor function and is an indication of the active expression of viral oncogene E7. For multivariate analysis of molecular mechanisms of oncogenic transformation, the obtained data of p16INK4a expression options were distributed according to the clinical and morphological characteristics of squamous cancers. Mixed nuclear-cytoplasmic expression of p16INK4a more than 5% of tumor cells was observed in 73.6% of cases, which confirms the relations of path of carcinogenesis of cancers of this localization with HPV. Statistically significant association between a positive p16INK4a status and decrease of the degree of of SCCO differentiation (p=0,002, r=-0,365) was determined. But with respect to other clinical and morphological characteristics of SCCO and marker p16INK4a statistically significant differences between groups were not found (p>0.05), this denies its prognostic and predictive value in the diagnosis of SCCO.

Author Biography

O. V. Poslavskaya

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
Department of pathological anatomy and forensic medicine
Dzerzhinsky str., 9, Dnipropetrovsk, 49044, Ukraine


Zaporozhan VM, Tsehelskyy MR. [Gynecological pathology: Atlas]. Odessa: Odessa medical university. 2002;308. Ukrainian.

Petrov SV, Reichlin NT. [Guide on immunohis-tochemical diagnosis of human tumors: 3-rd ed.]. Kazan: TItul. 2004;456. Russian.

Bradley KT, Budnick SD, Logani S. Immuno¬his-tochemical detection of p16INK4a in dysplastic lesions of the oral cavity. Modern рathology. 2006;19(2):1310-16.

Ernoux-Neufcoeur P, Arafa M, Decaestecker C, et al. Combined analysis of HPV DNA, p16, p21 and p53 to predict prognosis in patients with stage IV hypo-pharyngeal carcinoma. J. cancer res. clin. oncol. 2011;137:173-81.

Kumar B, Cordell K G, Lee JS, et al. EGFR, p16, HPV Titer, Bcl-xL and p53, sex, and smoking as indicators of response to therapy and survival in oro-pharyngeal cancer. Clin. oncol. 2008;26(19):3128-37.

Hafkamp HC, Manni JJ, Haesevoets A, et al. Mar¬ked differences in survival rate between smokers and nonsmokers with HPV 16-associated tonsillar carcinomas. Cancer. 2008;122(12):2656-64.

Lewis JSJr, Thorstad WL, Chernock RD, et al. p16 positive oropharyngeal squamous cell carcinoma:an entity with a favorable prognosis regardless of tumor HPV status. American journal of surgical pathology. 2010;34(8):1088-96.

Weinberger PM, Yu Z, Haffty BG, et al. Progno-stic significance of p16 protein levels in oropharyngeal squamous cell cancer. Clin. cancer res. 2004;10:5684-91.

Kuo KT, Hsiao CH, Lin CH, et al. The biomar¬kers of human papillomavirus infection in tonsillar squamous cell carcinoma-molecular basis and predicting favorable outcome. Mod. pathol. 2008;21(4):376-86.

Hoover AC, Spanos WC, Harris GF, et al. The role of human papillomavirus 16 E6 in anchorage-inde-pendent and invasive growth of mouse tonsil epithelium. Arch. otolaryngol head neck surg. 2007;133(5):495-502.

Barnes L, Eveson J, Richard P, et al. World Health Organization of tumours pathology and genetics of head and neck tumours. Lyon, France: IARC Press, 2005;430.




How to Cite

Poslavskaya OV. Features of expression of oncoprotein p16ІNK4a in squamous cell carcinomas of the oropharynx. Med. perspekt. [Internet]. 2016May31 [cited 2024Jun.13];21(2):69-73. Available from: https://journals.uran.ua/index.php/2307-0404/article/view/72154