Gene expression of nuclear factor of activated T-cells in children with bicuspid aortic valve.
DOI:
https://doi.org/10.26641/2307-0404.2016.3.82012Keywords:
bicuspid aortic valve, children, dopplerocardiography, gene expression, NFATC1, NFATC4Abstract
The genes of nuclear factor of activated T-cells (NFATC) play a key role both in heart valves formation and immune response. The genes belong to transcriptional factors and lead to myocardial hypertrophy in postnatal period that is confirmed by experimental models on animals. The purpose of this study was to reveale NFATC1 and NFATC4 genes expression level in the blood of 30 children with congenital heart diseases (CHD), 15 of them had bicuspid aortic valve, 15 had CHD without valve anomalies and 15 healthy children of the control group. No patients had signs of heart failure. At the first sage of Doppler heart ultrasound study a significant increase of the left ventricle posterior wall and ventricular septum thickness in comparison with CHD and control was revealed in BAV group. At the second stage a significant rise of relative normalized expression of NFATC1 in BAV children compared to CHD and control without differences in the expression of NFATC4 gene were established. Thus, in children with bicuspid aortic valve, formation of early myocardial hypertrophy with changes in nuclear factor of activated T-cells genes expression with prevailing of NFATC1 expression takes place. The detection of NFATC1 expression level in the blood of children could be considered as an early marker of progressive myocardial hypertrophy.
References
Polovkova OG. [The role of calcineurin signal pathway genes in myocardium remodeling of patients with ischemic heart disease]. Avtoref. cand. med. nauk. Tomsk. 2013;23. Russian.
Fedotova AG, Astratenkova IV, Linde EV, Or-dzho¬nikidze ZG, Akhmetov II. [Clinical and genetic as-pects of the "pathological athlete's heart" formation in highly skilled athletes]. Vestnik sportivnoy nauki. 2009;2:32-37. Russian.
Shen L, Li Z.Z, Shen A.D, Liu H, Bai S, Guo J, Yuan F, Li XF. Association of NFATc1 gene polymorphism with ventricular septal defect in the Chinese Han population. Chin Med J (Engl). 2013;126(1):78-81
Fernando Macian. Nfat proteins: key regulators of Т-cell development and function. Nature reviews. Im-munology. 2005;5:472-84.
Ida Gjervold Lunde. Molecular mechanisms of heart failure; Nuclear Factor of Activated T-cell (NFAT)signaling in myocardial hypertrophy and dysfunc-tion. Dissertation for the degree of Philosophiae Doctor (PhD), University of Oslo, Oslo, Norway. Series of dissertations submitted to the Faculty of Medicine, University of Oslo, 2011;1307.
Kenneth J, Livak and Thomas D. Schmittgen, Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2- ∆∆CT Method. METHODS. 2001;25:402-8.
Edgar Serfling, Andris Avots, Stefan Klein-Hes-sling, Ronald Rudolf, Martin Vaeth and Friederike Berberich-Siebelt Cell Communication and Signaling NFATc1/αA: The other Face of NFAT Factors in Lymphocytes. 2012;10:16.
de Frutos S, Caldwell E, Nitta CH, Kanagy NL, Wang J, Wang W, Walker MK, Gonzalez Bosc LV. NFATc3 contributes to intermittent hypoxia-induced arterial remodeling in mice. Am J Physiol Heart Circ Physiol. 2010;299(2):356-63.
de Frutos S, Duling L, Alò D, Berry T, Jackson-Weaver O, Walker M, Kanagy N, González Bosc L. NFATc3 is required for intermittent hypoxia-induced hypertension. Am J Physiol Heart Circ Physiol. 2008;294(5):2382-90.
Lin CJ, Lin CY, Chen CH, Zhou B, Chang CP. Partitioning the heart: Mechanisms of cardiac septation and valve development. Development. 2012;139:3277-99.
Poirier O, Nicaud V, McDonagh T, Dargie HJ, Desnos M, Dorent R, Roizès G, Schwartz K, Tiret L, Ko-majda M, Cambien F. Polymorphisms of genes of the cardiac calcineurin pathway and cardiac hypertrophy. Eur J Hum Genet. 2003;11(9):659-64.
Eva van Rooij, Pieter A Doevendans, Chiel C de Theije, Fawzi A Babiker, Jeffery D Molkentin, Leon J. De Wind Requirement of Nuclear Factor of Activated T-cells in Calcineurin-mediated Cardiomyocyte Hyper¬trophy. The Journal Of Biological Chemistry. 2002;277(50):48617.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2017 Medical Perspectives
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Submitting manuscript to the journal "Medicni perspektivi" the author(s) agree with transferring copyright from the author(s) to publisher (including photos, figures, tables, etc.) editor, reproducing materials of the manuscript in the journal, Internet, translation into other languages, export and import of the issue with the author’s article, spreading without limitation of their period of validity both on the territory of Ukraine and other countries. This and other mutual duties of the author and all co-authors separately and editorial board are secured by written agreement by special form to use the article, the sample of which is presented on the site.
Author signs a written agreement and sends it to Editorial Board simultaneously with submission of the manuscript.
