The correlation between individual biochemical parameters of functional state of the liver in expe¬rimental obstructive jaundice and course administration of L-lysine escinate and glutargine.

Authors

  • V. I. Desjateryk
  • D. V. Mamchur
  • O. V. Kotov
  • S. M. Dronov
  • V. I. Zhyliuk

DOI:

https://doi.org/10.26641/2307-0404.2016.4.89895

Keywords:

experimental obstructive jaundice, superoxide dismutase, malonic dialdehyde, oxidative modification of proteins, bilirubin, hepatic transaminases, correlation

Abstract

The aim of this study was to determine the correlations between markers of oxidant-antioxidant stress in the blood of experimental animals, as well as the content of bilirubin and activity of enzymes in experimental obstructive jaundice. Studies were conducted on 84 adult male Wistar rats (180-230 g). Animals were divided into 4 groups: intact (n = 12), control (n = 24), L-lysine escinate (0,5 mg/kg, n = 24) and glutargin (40 mg/kg, n = 24). The isolation and ligation of the common bile duct below confluence was carried out under general anesthesia. The test preparations were administered 1 time per day, intraperitoneally. Evaluation of the treatment efficacy was carried out on 4th and 10th day of the study. The content of malonic dialdehyde (MDA), aldehyde- (APH) and ketone-phenylhydrazones (CPH), bilirubin and the activity of superoxide dismutase (SOD), catalase, aspartate aminotransferase (ALT) and alanine aminotransferase (AST) in the blood were determined using conventional techniques. The correlation links between common markers (bilirubin, AST, ALT) and indicators characterizing oxidant-antioxidant stress (MDA, APH, CPH, SOD, catalase) were determined in the study. High rate of correlation between them was established. The results allow to evaluate indirectly the intensity of oxidative metabolism processes in the liver, which can be used in clinical practice to assess the severity degree of the patients with progressive obstructive jaundice.

Author Biographies

V. I. Desjateryk

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
Dzerzhinsky str., 9, Dnipro, 49044, Ukraine

D. V. Mamchur

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
Dzerzhinsky str., 9, Dnipro, 49044, Ukraine

O. V. Kotov

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
Dzerzhinsky str., 9, Dnipro, 49044, Ukraine

S. M. Dronov

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
Dzerzhinsky str., 9, Dnipro, 49044, Ukraine

V. I. Zhyliuk

SE «Dnipropetrovsk medical academy of Health Ministry of Ukraine»
Dzerzhinsky str., 9, Dnipro, 49044, Ukraine

References

Gal'peryn EY, Kotovskyj AE, Momunova ON. [Rate of biliary ducts’ decompression by the tumorous obstructive jaundice]. Hyrurgyja. 2011;8:33-40. Russian.

Goncharenko MS, Latinova AM. [Method for eva¬luation of lipid peroxidation]. Laboratornoe delo. 1985;1:60-61. Russian.

Desjateryk VI, Mamchur DV. [Prophylaxis of hepatic insufficiency in patients with mechanical jaun-dice]. Klinichna hirurgija. 2014;3:60-64. Ukrainian.

Davydov VG, Boychuk SV, Shaymardanov RSh, Valeyeva IKh, Minnebayev MM. [Quantitative asses-sment of hepatocytes destruction and biochemical changes of blood and bile at experimental mechanical jaundice]. Rossyjskyj zhurnal gastroenterologyy, gepa-tologyy, koloproktologyy. 2007;18(1):25-31. Russian.

Koroljuk MA, Yvanova LY, Majorova YG, To-karev VE. [Method for determining catalase activity]. La-boratornoe delo. 1988;1:16-19. Russian.

Nychytajlo MJu, Godlevs'kyj AI, Savoljuk SI. [Critical postoperative period in patients with non-tumor obstructive jaundice after surgical internal biliary decompression]. Harkivs'ka hirurgichna shkola. 2012;3:71-74. Ukrainian.

Laptev VV, Rumiantseva SA, Tskayev AYu, Gi-virovska NE, Cherniakov AV, Smirnova NA, Malko-va EV. [Administration of Hepa-Merz Preparation in Treat¬ment of Surgical obstructive Jaundice of Benign Genesis]. Liky Ukrai'ny. 2010;139:87-91. Russian.

Chevary S, Chaba I, Sekei I. [The role of super-oxide dismutase in cellular oxidative processes and methods for its estimation in biological materials]. La-boratornoe delo. 1985;11:678-681.

Halliwell B. Free radical in Biology and Medicine / B. Halliwell, M.C. Yutteridge. Oxford: Cla¬rendon Press. 1999;320.

Downloads

Published

2016-12-22

How to Cite

1.
Desjateryk VI, Mamchur DV, Kotov OV, Dronov SM, Zhyliuk VI. The correlation between individual biochemical parameters of functional state of the liver in expe¬rimental obstructive jaundice and course administration of L-lysine escinate and glutargine. Med. perspekt. [Internet]. 2016Dec.22 [cited 2024Nov.22];21(4):8-15. Available from: https://journals.uran.ua/index.php/2307-0404/article/view/89895

Issue

Vol. 21 No. 4 (2016)

Section

THEORETICAL MEDICINE

License

Copyright (c) 2017 Medical Perspectives

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Submitting manuscript to the journal "Medicni perspektivi" the author(s) agree with transferring copyright from the author(s) to publisher (including photos, figures, tables, etc.) editor, reproducing materials of the manuscript in the journal, Internet, translation into other languages, export and import of the issue with the author’s article, spreading without limitation of their period of validity both on the territory of Ukraine and other countries. This and other mutual duties of the author and all co-authors separately and editorial board are secured by written agreement by special form to use the article, the sample of which is presented on the site.

Author signs a written agreement and sends it to Editorial Board simultaneously with submission of the manuscript.