Ethelial-mesenchymal transformation of papillary thyroid mcrocarcinomas
Keywords:papillary thyroid microcarcinomas, epithelial-mesenchymal transformation, lymph nodes metastases
Epithelial-mesenchymal transformation (EMT) in papillary thyroid cancer results in high risk of invasion and lymph node metastasizes. The aim of the study was to investigate the immunohistochemical features of EMT in papillary thyroid microcarcinomas (PTM) for identify morphological diagnostic features of tumor aggressive clinical behavior.
Methods. The immunohistochemical expression patterns of vimentin were examinated in central regions, invasion zones and lymph node metastases in 28 patients with PTM. It is investigated an expression of NIS, VEGF, MMP-9 and pan-cytokeratin in PTM with solid/trabecular pattern of growth and high level expression of vimentin.
Results. It is shown that microcarcinomas with high level expression of vimentin, exhibiting a solid/trabecular pattern of growth associated with increase MMP-9 (p<0,005), VEGF (р<0,05), and decrease NIS (р<0,005) level expression. These changes reflect phenotype of the epithelial-mesenchymal transformation and associated with the presence of metastases in cervical lymph nodes (p<0,001).
Conclusions. Epithelial-mesenchymal transformation which can be seen under routine H&E observation is an important indicator of lymph node metastasis of PTM.
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Copyright (c) 2015 Ирина Ивановна Яковцова, Игорь Владимирович Ивахно, Оксана Владимировна Долгая, Анрей Евгениевич Олейник, Светлана Владимировна Данилюк
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