Effect of chlorpyrifos on some physiological, biochemical and functional properties of rats blood hаemoglobin


  • Володимир Петрович Росаловський Institute of animal biology NAAS, V. Stus street, 38, Lviv, Ukraine, 79034, Ukraine




chlorpyrifos, rats, red blood cells, hemoglobin, catalase, superoxide dismutase


We studied how chlorpyrifos affects the dynamics of hemoglobin oxygen saturation, the ratio of ligand hemoglobin forms, catalase and superoxide dismutase activity in hemolyzed erythrocytes of peripherical rat blood during an hour after exposure. We found a significant decrease in hemoglobin affinity to oxygen and increased methemoglobin at 15 min after chlorpyrifos exposure

Author Biography

Володимир Петрович Росаловський, Institute of animal biology NAAS, V. Stus street, 38, Lviv, Ukraine, 79034

junior researcher


Salyha, Y. (2010). Biological effects assessment of chlorpyrifos and some aspects of its neurotoxicity.Visnyk of Lviv University. Biology series, 54, 3–14.

Vlizlo, V. V., Salyha, Y. T. (2012). Some problems of biological safety of aplication of pesticides in Ukraine. News of agrarian sciences, 1, 24–27.

Jin, Y., Liu, Z., Peng, T., Fu, Z. (2015). The toxicity of chlorpyrifos on the early life stage of zebrafish: A survey on the endpoints at development, locomotor behavior, oxidative stress and immunotoxicity. Fish & Shellfish Immunology, 43 (2), 405–414. doi: 10.1016/j.fsi.2015.01.010

Rosalovsky, V., Salyha, Y. (2013). New biochemical and physiological aspects of chlorpyrifos neurotoxicity. Toxicology Letters, 221, 200. doi: 10.1016/j.toxlet.2013.05.468

Whitney, K. D., Seidler, F. J., Slotkin, T. A. (1995). Developmental neurotoxicity of chlorpyrifos: cellular mechanisms.Toxicol Appl Pharmacol, 134 (1), 53–62. doi: 10.1016/j.toxlet.2013.05.468

Salyha, Y. T. (2013). Chlorpyrifos Leads to Oxidative Stress-Induced Death of Hippocampal Cells in Vitro. Neurophysiology, 45 (3), 193–199. doi: 10.1007/s11062-013-9356-7

Salyha, N. O. (2013). Activity of the glutathione system of antioxidant defense in rats under the action of L-glutamic acid Ukr. Biochem J., 85 (4), 40–47.

Salyha, Y. T. (2013). Antioxidant system indices in brain of rats intoxicated by chlorpyrifos. Studia Biologica, 7 (3), 85–96

Dudok, K. P., Vlokh, I. J., Vlokh, R. O. (2010). Structural and functional state of the erythrocytes, ligand forms of haemoglobin and content of molecules of middle mass in blood plasma of patients with schizophrenia disorders Studia Biologica, 4 (1), 15–26.

Dotsenko, O. I., Dotsenko, V. А., Mishchenko,A. M. (2010). The activity of erythrocytes superoxide dismutase and catalase and some other tissues at condition of low frequency vibration. Fhysics of Alive, 18 (1), 107–113.

Salyha, Yu. T., Tolokha, N. I., Stefanyshyn, O. M. (2011). Some parameters of antioxidant system in erythrocytes of rats under the chronic influence of chlorpyrifos. Medical i Clinical Chemistry, 13 (4), 103–106.

Lukaszewicz-Hussain, А. (2001). Organophosphate insecticide chlorfenvinphos affects superoxide dismutase, catalase and malondialdehyde in rat liver. Pol. J. of Environm, 10 (4), 279–282.

Ivanov, Y. T. (1975). Modification of spectrophotometric method for determining the curves of oxygen-hemoglobin dissociation. Bull.of experiment. Biology and medicine, 79 (11), 122–128.

Bilyy, O. I., Dudok, K. P., Lukyanets, V. (1998). Determination of hemoglobin and its ligand forms in whole blood by absorption spectroscopy. Lviv, 12.

Vlizlo, V. V., Fedoruk, R. S., Ratycz, I. B. et al. (2012). Laboratory methods of research in biology, stockbreeding and veterinary medicine. Lviv, SPOLOM, 761.

Vlizlo, V. V., Fedoruk, R. S., Makar, I. A. et al. (2004). Physiological and biochemical methods of research in biology, stockbreeding and veterinary medicine. Lviv, 399.

Lowry, O. H. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193 (1), 265–275.

Salyha, Y. T., Rosalovsky, V. P. (2012). To the study of some parameters of antioxidant defense system and lipid peroxidation in blood of rats under the toxic influence of chlorpyrifos. Ukr morph. alm., 10 (3), 94–95.

Salyha, N., Salyha, Yu. (2013). Effect of L-glutamic acid on the activity of glutathione metabolism enzymes and intencity of peroxidation processes in rats Visn. Lviv Univ. Biol. Ser. 62, 61–67.

Salyha, Yu., Rosalovsky, V., Fedyakov, R. (2012). Glutathione system in erythrocytes of rats intoxicated by chlorpyrifos. Visn. Lviv Univ. Biol. Ser., 60, 99–104.





Biological sciences