Determination of nitrates toxcity using cytomorphologic parametres of various tissues of fish as a biomarker

Authors

  • Майя Розметовна Верголяс Institute of colloid chemistry and water chemistry named A. V. Dumansky NAS Ukraine 42 Acad. Vernadsky boul., Kyiv, Ukraine, 03680, Ukraine

DOI:

https://doi.org/10.15587/2313-8416.2015.49341

Keywords:

drinking water, nitrates, genotoxicity, cytotoxicity, WBC fish blood

Abstract

A biological analysis of aqueous samples containing different concentrations of nitrates is conducted. An influence of nitrates on the organism and the cells of fish is described. Data were obtained about the negative influence of maximum permissible concentration of nitrates in the water. It has been shown that the toxic effect of nitrates on an organism of fish is changing the quantitative composition of peripheral blood leukocytes, and observed the structural changes of cell nuclei in various tissues. It was found that the nitrates contained in the water samples have cyto-and genotoxic effect

Author Biography

Майя Розметовна Верголяс, Institute of colloid chemistry and water chemistry named A. V. Dumansky NAS Ukraine 42 Acad. Vernadsky boul., Kyiv, Ukraine, 03680

Candidate of Biology Sciences

senior researcher, head of laboratory

References

Levich, A. P., Bulgakov, N. G., Maksimov, V. N. (2004). Theoretical and methodological foundations of regional technology control environment according to ecological monitoring. Moscow, 271.

Panichev, K. V., Serednyak, V. G., Karzhan, M. V. (2000). Intoxication metgemoglobinoobrazovatelyami poisoning in children. Anesthesiology. and reanimatol, 56–58.

Gupta, S. K., Gupta, R. C., Gupta, A. B., Seth, A. K., Bassin, J. K., Gupta, A. (2000). Recurrent Acute Respiratory Tract Infections in Areas With High Nitrate Concentrations in Drinking Water. Environmental Health Perspectives, 108 (4), 363–366. doi: 10.1289/ehp.00108363

Patika, V. P., Makarenko, N. A., Moklyachuk, L. I.; Patiki, V. P. (Ed.) (2005). Agroekologіchna otsіnka Welcome mіneralnih that pestitsidіv. Kyiv, 300.

Zhukov, G. F., Filin, S. A., Zaitsev, A. N., Mamonov, L. G. (1991). Of nitrate in the composition of the diet of young children. Nutrition, 6, 49–53.

Vergolyas, M. R., Kucherenko, T. V., Arhipchuk, V. V. (2007). Comparative Analysis of Incidence of cells with micronuclei and dual cores had carp Carassius auratus in natural and laboratory conditions. Dosyagnennya problems of genetics, selektsіi that bіotehnologіi. Kyiv, 1, 203–206.

Goncharuk, V. V., Vergolyas, M. P. (2009). Sposіb viznachennya tsitotoksichnostі Water seredovischa, Pat. 85,493 Ukraine: the IPC G 01N 33/18., the applicant and the patentee, Kiev, Institute of Colloid and Water Chemistry, National Academy of Sciences of Ukraine, 200801532; zayavl.06.02.08; publ. 26.01.09, Bul. 2, 4.

Norppa, H. (2003). What do human micronuclei contain? Mutagenesis, 18 (3), 221–233. doi: 10.1093/mutage/18.3.221

Vergoljas, M. R., Goncharuk, V. V. (2008). Vykorystannja cytologichnyh biomarkeriv na rybah dlja ocinky antropogennogo zabrudnennja mors'kyh i prisnyh vod. Faktory eksperymental'noi' evoljucii' organizmiv. Kyiv: LOGOS, 4, 60–63.

Goncharuk, V. V., Vergolyas, M. R., Boltіna, І. V. (2011). Sposіb viznachennya genotoksichnosti Water seredovischa. Pat. 95,717 Ukraine: the IPC G 01,33/18. VV,; the applicant and the patentee, Kiev, Institute of Colloid and Water Chemistry, National Academy of Sciences of Ukraine. 201004569; zayavl.19.04.10; publ. 25.08.11, Bul. 16, 3.

Arkhipchuk, V. V., Garanko, N. N. (2005). Using the nucleolar biomarker and the micronucleus test on in vivo fish fin cells. Ecotoxicology and Environmental Safety, 62 (1), 42–52. doi: 10.1016/j.ecoenv.2005.01.001

Hayashi, M., Ueda, T., Uyeno, K., Wada, K., Kinae, N., Saotome et. al (1998). Development of genotoxicity assay systems that use aquatic organisms. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 399 (2), 125–133. doi: 10.1016/s0027-5107(97)00251-0

Lakin, G. F. (1980). Biometrics. Moscow: Higher, 293.

Ivanova, N. T. (1983). Atlas blood cells of fish. Moscow: Light and food industries, 184.

Vergolyas, M. R., Bezrukov, V. F., Manilo L. G. (2007).Tsitologіchna characteristic periferichnoї krovі dev'yati vidіv RIB. Suchasnі problemi bіologії, ekologії that hіmії. Zaporizhya, 2, 217–220.

SanPyN 2.1.4.1175-02 «Gygyenycheskye trebovanyja k kachestvu vody necentralyzovannogo vodosnabzhenyja. Sanytarnaja ohrana ystochnykov» (2003). Canytarno-epydemyologycheskyepravyla y normatyvy. Moscow.

DSTU 7527 (2014). Water Pitney. Vimogi that methodological kontrolyuvannya of Quality. Enter 2014-02-01. Kyiv: DP UkrNDNC, 10.

Published

2015-09-28

Issue

Section

Biological sciences