The state of bronchi remodeling in school-age children with bronchial asthma at the Glutathione-S-Transferase gene polymorphism
DOI:
https://doi.org/10.15587/2313-8416.2016.59327Keywords:
bronchial asthma, bronchi remodeling, glutathione-s-transferase, gene polymorphism, school childrenAbstract
The aim of research was to establish connection between the bronchi remodeling processes and allelic polymorphism of GSTT1 and GSTM1 genes in school-age children with bronchial asthma (BA) for optimization of results of the basic treatment.
Methods: 66 school children with bronchial asthma in the period without attacks underwent the complex examination. All patients underwent general clinic and spirographic examination, point assessment of the bronchial asthma controllability with the help of clinically-instrumental evaluation scale, the analysis of the sample of capillary blood by the method of multiplex polymerase chain reaction (PCR) for detecting the deletions in glutathione-s-transferase genes that is GSTT1 and GSTM1.
Results: As the result of molecular and genetic analysis of studying of GSTT1 and GSTM1 genes polymorphism there were demonstrated that the (GSTT1+М1+) genotype homozygous on the normal copies was more often and took place in 40,9% of children, «null genotype» -in 9 patients (13,64 %), GSTT1-М1+ genotype was equally often, whereas the heterozygous GSTT1+М1- genotype was detected in every third patient (31,82 %).
Conclusions: The deletion polymorphism of GSТT1 and GSТM1 in homozygous state (so called “null genotype” is three times less often in school children with bronchial asthma comparing with patients with GSTT1+М1+ genotype, it raises more than twice the risk of the heavy clinical course of disease, associates with the low indices of bronchi lability. In patients with bronchial asthma even at preserved structure of glutathione-s-transferase genes (GSTT1+М1+ genotype) the continuous contact with the tobacco smoke in family raises the content of endothelial factor of vessel growth (EFVG) in sputum in 1,25 times that underlines the more aggressive remodeling of respiratory tracts
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Copyright (c) 2016 Галина Анатоліївна Білик, Олена Костянтинівна Колоскова
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