Визначення L-Аргініну у крові щурів ензиматичним методом
DOI:
https://doi.org/10.15587/2313-8416.2015.54486Słowa kluczowe:
Аргінін, сечовина, кров щурів, ензиматично-хімічний аналіз, аргіназа, 2, 3-бутандіонмонооксимAbstrakt
Здійснено опрацювання ензиматично-хімічного методу кількісного аналізу L-Аргініну (Арг) на реальних зразках крові щурів. Розроблений метод ґрунтується на використанні рекомбінантної аргінази І та 2,3-бутандіонмонооксиму (ДМО). Продукт реакції (сечовина) реєструється флуорометрично. Показано придатність нового методу для визначення Арг в біологічних рідинах ссавців
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