INFLUENCE OF DYNAMIC LOADS DIFFERENT IN DURATION ON CHANGES IN THE ACTIVITY OF LACTATE DEHYDROGENASE ISOENZYMES IN INTERVERTEBRAL DISC CELLS

Andrіі Sak; Raisa Antypova

Authors

Andrіі Sak Kharkiv State Academy of Physical Culture, https://orcid.org/0000-0002-8491-3434
Raisa Antypova Kharkiv State Academy of Physical Culture, https://orcid.org/0000-0002-7172-4597

Keywords:

lactate dehydrogenase, intervertebral disc, treadmill, experimental running.

Abstract

Purpose: to determine the effect of long-term dynamic loads on changes in the activity of isoenzymes lactate dehydrogenase (LDH) in the cells of the intervertebral discs in the age aspect.
Material and Methods: the study was performed on male Wistar rats of three age groups: 1, 3 and 12 months. A total of 90 animals of the experimental series and 90 animals of the control series were observed. Dynamic loads were created by running in a horizontal treadmill. Using histochemical methods, the analysis of changes in the activity of lactate dehydrogenase isoenzymes in the cells of the intervertebral discs of Wistar rats after 20- and 90-day experimental run was performed.
Results: it is experimentally established that changes in the activity of lactate dehydrogenase isoenzymes depend on the age of the animals and the duration of dynamic loads. There was a decrease in the activity of LDH-1 and LDH-2 isoenzymes in the cells of the intervertebral disc, but an increase in the activity of LDH-3 and LDH-4 isoenzymes.
Conclusions: the identified age features of changes in enzyme activity are proposed to be used as a histochemical test to objectively assess the degree of destructive effect of prolonged running on the fibrous cartilage of the intervertebral disc.

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INFLUENCE OF DYNAMIC LOADS DIFFERENT IN DURATION ON CHANGES IN THE ACTIVITY OF LACTATE DEHYDROGENASE ISOENZYMES IN INTERVERTEBRAL DISC CELLS

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