Effect of transplanted neural progenitors on hippocampal cells proliferation after ischemic brain injury

Authors

DOI:

https://doi.org/10.15587/2519-4798.2017.105502

Keywords:

neural progenitor cells, cells transplantation, brain ischemia, hippocampus, cells proliferation

Abstract

The aim of research was to establish the effect of neural progenitors (NP) transplantation on hippocampal cells proliferation after an ischemic brain injury of a mouse.

Methods. An ischemic brain injury was modeled by 20-minutes occlusion of both carotid arteries in mice of FVB line with the further reperfusion. In 24 hours after the occlusion NP, separated from the hippocampus of mice of FVB-Cg-Tg(GFPU),5Nagy/J line, transgenous by a green fluorescent protein (GFP) gen, were stereotaxically transplanted in CA1 hippocampus zone of ischemic animals. To reveal proliferating cells, all animals from experimental groups were intaabdominally administered with synthetic nucleoside 5-bromodeoxyuridine (BrdU). For the assessment of hippocampal cells proliferation after the ischemic brain injury and after NP transplantation, the immunohistochemical coloration of brain cuts was realized using antibodies against BrdU.

Results. The received data demonstrated that NP transplantation after an ischemic brain injury statistically reliably increases the number of BrdU-positive cells in the dentate gyrus comparing with animals from the control pseudo-operated group and the comparison group without the transplantation.

Conclusions. These data allow to suppose that NP transplantation after an ischemic brain injury may influence cells proliferation in the subgranular zone of the dentate gyrus and in such a way stimulate the neurogenesis in the hippocampus

Author Biographies

Oleg Tsupykov, Bogomoletz Institute of Physiology of NAS of Ukraine Bohomoltsya str., 4, Kyiv, Ukraine, 01024

PhD, Leading Researcher

Department of Cytology 

Vitalii Kyryk, State Institute of Genetic and Regenerative Medicine National Academy of Medical Sciences of Ukraine Vyshgorodska str., 67, Kyiv, Ukraine, 04114

PhD, Head of Laboratory

Laboratory of Cell and Tissue Cultures

Kateryna Yatsenko, Bogomoletz Institute of Physiology of NAS of Ukraine Bohomoltsya str., 4, Kyiv, Ukraine, 01024

PhD, Senior Researcher

Department of Cytology

Gennadii Butenko, State Institute of Genetic and Regenerative Medicine National Academy of Medical Sciences of Ukraine Vyshgorodska str., 67, Kyiv, Ukraine, 04114

Academician of NAS of Ukraine, Academician of NAMS of Ukraine, Director 

Galyna Skibo, Bogomoletz Institute of Physiology of NAS of Ukraine Bohomoltsya str., 4, Kyiv, Ukraine, 01024

MD, Head of Department

Department of Cytology 

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Published

2017-06-30

How to Cite

Tsupykov, O., Kyryk, V., Yatsenko, K., Butenko, G., & Skibo, G. (2017). Effect of transplanted neural progenitors on hippocampal cells proliferation after ischemic brain injury. ScienceRise: Medical Science, (6 (14), 32–36. https://doi.org/10.15587/2519-4798.2017.105502

Issue

No. 6 (14) (2017)

Section

Medical Science

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Copyright (c) 2017 Oleg Tsupykov, Vitalii Kyryk, Kateryna Yatsenko, Gennadii Butenko, Galyna Skibo

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