Enterosgel role in neurodegenerative changes in the retina rats under the influence of Cr (VI) - induced retinopathy by study morphological changes in dynamic

Authors

DOI:

https://doi.org/10.15587/2519-4798.2021.219921

Keywords:

hexavalent chromium, retina, toxicity experiment, enterosgel, morphology, morphometry

Abstract

Chromium galvanic production have leaded to biosphere pollution. Therefore advisable to study of role in the neurodegenerative development in retinal diseases under experimental conditions.

The aim is to study the Enterosgel effect on morphological changes in rats retina with Cr(VI) – induced retinopathy.

Materials and methods. An experimental study had carried out on 72 outbred white male rats. The rats had divided into 3 groups: I – control group of intact rats (n = 24). Control rats were received drinking water, II group – rats (n = 24), were received drinking water with Cr (VI) (K2Cr2O7) – 0.02 mol/L, III group – animals (n = 24) were received drinking water with K2Cr2O7– 0.02 mol/L and hydrogel methylsilicic acid (Enterosgel) at a dose of 0.8 mg/kg per day as a corrector. The animals had been decapitated under ether anesthesia. The retina had been studied on days 20, 40 and 60 of the experiment. Morphologically and morphometrically they had analyzed.

Results. According to histological studies, it has proved that Cr (VI) causes dystrophic and degenerative changes in all rats retina layers. They increase as the duration of the experiment. The use of Enterosgel as a corrective therapy showed positive results in restoring the morphological structure of rats retina. After Enterosgel 20 days using as a corrector of Cr (VI) exposure, there is a barely noticeable swelling of the outer and inner nuclear layers. Other layers of the retina, morphologically, look undamaged. Forty days Enterosgel treatment have outer and inner nuclear layer edema of retina of animals persists but does not increase. It is easy noticeable swelling of the outer and inner layers of mesh, but no signs of damage processes of cell populations nuclear layers. State ganglionic layer and nerve fiber layer entirely satisfactory. These pathological changes are not critical. After 60 days from the beginning of loading of Cr (VI) and application of Enterosgel in the retina of rats there are initial degenerative changes in the photosensory layer. Cystic dilated outer segments of rods and cones were visible throughout, and areas of their fragmentation were observed. Ganglion neurons are not damaged, but their axons appear somewhat thickened and fluffy. But in general, the typical structure of the retina is preserved.

Conclusions. Chromium-induced toxicity in rats is characterized by pronounced histological and morphometric changes and retinal thickness, which appear after 20 days, increase by 40 days and acquire maximum transformations after 60 days of the experiment. The use of Enterosgel improves picture morphological structures of the retina in rats under the influence of Cr (VI). The changes were expressed on days 20 and 40, which indicates the presence of protective properties for the retina

Author Biographies

Olena Kuzenko, Kharkiv Medical Academy of Postgraduate Education

Postgraduate Student

Department of Ophthalmology

Yevhen Kuzenko, Sumy State University

MD, Associate Professor

Department of Pathological Anatomy

Yuri Demin , Kharkiv Medical Academy of Postgraduate Education

MD, Professor

Department of Ophthalmology

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Published

2021-01-30

How to Cite

Kuzenko, O., Kuzenko, Y., & Demin , Y. (2021). Enterosgel role in neurodegenerative changes in the retina rats under the influence of Cr (VI) - induced retinopathy by study morphological changes in dynamic. ScienceRise: Medical Science, (1 (40), 14–20. https://doi.org/10.15587/2519-4798.2021.219921

Issue

No. 1 (40) (2021)

Section

Medical Science

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Copyright (c) 2021 Елена Владимировна Кузенко, Евгений Викторович Кузенко, Юрий Альбертович Дёмин

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