THE EFFECT OF PREPREVIOUS AND PRESUSPENDED INOCULATION OF SEEDS BY AZOTOBACTER VINELANDII 12M ON MAIZE PRODUCTIVITY AND ACTIVITY OF NITROGEN FIXATION PROCESS
DOI:
https://doi.org/10.33730/2310-4678.1.2020.203941Keywords:
maize, strain Azotobacter vinelandii 12, predecessor, chernozem typical, inoculation, nitrogen fixation, yieldAbstract
Recent years in Ukraine are characterized by an increase in acreage under maize. The attention of agricultural producers is focused on the application of the latest technologies and intensification of production, which will allow not only to increase the yield, but also to improve the quality of grain. Traditional maize cultivation technology involves the introduction of high doses of mineral fertilizers and chemical plant protection products. However, breaches of technology, neglect of recommendations and insufficient scientific justification can cause dangerous environmental impacts for agroecosystems. Therefore, more and more often in modern technologies of cultivation of agricultural crops it is possible to find ecologically safe elements based on the use of biological preparations. The effect of different types of precursors and pre-sowing seed inoculation of Azotobacter vinelandii 12M strain on nitrogen fixation activity and maize productivity was determined in the interim field experiment. It was found that the conditions formed in the chernozem typical after growing legumes are favorable for the functioning of the native nitrogenfixing microbiota and the survival of the introduced strain Azotobacter vinelandii 12M. In the rhizosphere of maize plants the level of nitrogen fixation activity was higher by 3% in the variant where the clover was predecessor and by 17% where the peas were, compared with the same variants, but without inoculation. The complex of properties possessed by the strain Azotobacter vinelandii 12M, namely the ability to absorb inert molecular nitrogen, the production of phytohormones and the manifestation of antagonism against phytopathogens, made it possible to obtain maize grain yields of 6.79 and 6.88 t/ha, respectively. clover respectively. Protein content increased by 0.3–0.5%, fat (by 0.2%) and starch (by 2–3%). In the rhizosphere of maize plants grown after winter wheat, the activity of the nitrogen fixation process was half that of the leguminous precursors. The yield and quality of the obtained grain in the variants of constant cultivation of maize and cultivation after winter wheat were lower by 0.1–1% than after leguminous precursors.
References
State Statistics Service of Ukraine. URL: http://ukrstat.gov.ua
Voronin, V.I., Stulin, A.F., Blekanov, D.N., Podrezov, P.I., Drachev, N.A. (2017). Otsenka produktivnosti kukuruzy v usloviyakh vyrashchivaniya eye v sevooborote i v vide monokultury pri dlitelnom primenenii udobreniy [Evalution of valuation of maize productivity in conditions of its crop rotation and monoculture with long-term fertilizer application]. Uspekhi sovremennoy nauki [Advances in Modern Science]. 7: 18–25. [In Russ.].
Stakhurlova, L.D., Stulin, A.F., Gromovik, A.I. (2015). Produktivnost kukuruzy v usloviyakh dlitelnogo primeneniya razlichnykh agrotekhnicheskikh priyemov na chernozemakh vyshchelochennykh [The productivity of corn under the conditions of prolonged use of various agricultural techniques on leached chernozems]. Vestnik VGU [Bulletin of the Voronezh State University]. 2: 92–95. [In Russ.].
Makarov, R.F., Arkhipova, V.V. (1997). Udobreniya i produktivnost kukuruzy [Fertilizers and corn productivity]. Kukuruza i sorgo [Corn and sorghum]. 3: 5–6. [In Russ.].
Demyanyuk, О.S., Shatsman, D.O. (2019). Ahroekolohichna ta ekonomichna otsinka zastosuvannia gruntovykh i strakhovykh herbitsydiv pry vyroshchuvanni kukurudzy na zerno v umovakh Livoberezhnoho Lisostepu Ukrainy [Agroecological and economic assessment of the application of soil and post emergent herbicides in the cultivation of corn for grain in the conditions of the left-bank forest-steppe of Ukraine]. Zbalansovane pryrodokorystuvannia [Balanced nature using]. 2: 57–64. [In Ukr.].
Van Peer, P., Punte, H.L.M., De Weger, L.A., Schippers, B. (1990). Characterization of root surface and endorhizosphere Pseudomonas in relation to their colonization of roots. Appl. Environ. Microbiol. 56: 2462–2470.
Normander, B., Prosser, J.I. (2000). Bacterial origin and community composition in the barley phytosphere as a function of habitat and presowing conditions. Appl. Environ. Microbiol. 66(10): 4372–4377.
Van Overbeek, L.S., Van Elsas, J.D. (1995). Root exudateinduced promoter activity in Pseudomonas fluorescens mutants in the wheat rhizosphere. Appl. Environ. Microbiol. 61: 890–898.
Boronin, A.M. (1998). Rizosfernyye bakterii roda Pseudomonas, sposobstvuyushchiye rostu i razvitiyu rasteniy [Rhizospheric bacteria of the genus Pseudomonas, contributing to the growth and development of plants]. Sorosovskiy obrazovatelnyy zhurnal [Soros educational journal]. 10: 25–32. [In Russ.].
Symochko, L., Demyanyuk, О. (2018). Mikrobiom gruntu kulturnykh roslyn za riznykh ahrotekhnolohii [Soil microbiome of cultural plants under different agrotechnologies]. Ahroekolohichnyi zhurnal [Agroecological journal]. 2: 87–92. [In Ukr.].
Mishustin, E.N., Shilnikova, V.K. (1968). Biologicheskaya fiksatsiya molekulyarnogo azota [Biological fixation of molecular nitrogen]. Moskva: Nauka. 530. [In Russ.].
Andreyuk, E.I., Valagurova, E.V. (1992). Osnovy ekologii pochvennykh mikroorganizmov [Fundamentals of ecology of soil microorganisms]. Kyiv: Naukova dumka. 224. [In Russ.].
Kots, S.Ya., Morgun, V.V., Patyka, V.F. еt al. (2014). Biologicheskaya fiksatsiya azota: assotsiativnaya azotfiksatsiya: monografiya. T. 4 [Biological nitrogen fixation: associative nitrogen fixation: monograph]. Kyiv: Logos: 412. [In Russ.].
Morgun, V.V., Kots, S.Ya., Kirichenko, O.V. (2009). Roststimuliruyushchiye rizobakterii i ikh prakticheskoye primeneniye [Growth-promoting rhizobacteria and their practical use]. Fiziologiya i biokhimiya kulturnykh rasteniy [Physiology and biochemistry of cultivated plants]. 41(3): 187–207. [In Russ.].
Kirichenko, E.V., Titova, L.V., Kots, S.Ya. (2010). Effektivnost bakterizatsii semyan pshenitsy yarovoy novym shtammom Azotobacter chroococcum T79 [The effectiveness of bacterization of wheat seeds in spring with a new strain of Azotobacter chroococcum T79]. Agrarna nauka [Agricultural science]. 1: 21–24. [In Russ.].
Sherstoboeva, О.V., Vaha, L.I. (2012). Bioriznomanittia ta antahonistychna aktyvnist bakterii rodu Azotobacter u gruntakh Lisostepu Ukrainy [Biodiversity and antagonistic activity of bacteria of the genus Azotobacter in the forest-steppe soils of Ukraine]. Ahroekolohichnyi zhurnal [Agroecological journal]. 1: 61–64. [In Ukr.].
Sherstoboeva, О.V., Vaha, L.I. (2012). Vplyv systemy udobrennia na biolohichnu aktyvnist shtamiv azotobaktera z gruntu ahrofitotsenozu pshenytsi ozymoi [Influence of fertilizer system on biological activity of azotobacter strains from winter wheat agrophytocenosis]. Zbalansovane pryrodokorystuvannia [Balanced nature using]. 1: 79–83. [In Ukr.].
Tejera, N., Lluch, C., Martínez-Toledo, M.V., González-López, J. (2005). Isolation and characterization of Azotobacter and Azospirillum strains from the sugarcane rhizosphere. Plant and Soil. 270(1–2): 223–232.
Bertsova, Yu.V., Demin, O.V., Bogachev, A.V. (2005). Dykhatelnaya zashchita nitrogenaznogo kompleksa u Azotobacter vinelandii [Respiratory protection of the nitrogenase complex in Azotobacter vinelandii]. Uspekhi biologicheskoy khimii [Advances in biological chemistry]. 45: 205–234. [In Russ.].
Eckert, В., Weber, O.B., Kirchhof, G. еt al. (2001). Azospirillum dobereinerae sp. nov., a nitrogen-fixing bacterium associated with the C4-grass Miscanthus. Intern. J. Syst. Evol. Microbiol. 51(1): 17–26.
Dospekhov, B.A. (1985). Metodyka polevoho opyta [Methodology of the field experience]. Moskva: Kolos: 351. [In Russ.].
Sherstoboeva, O.V., Fedak, L.I. (2009). Polifunktsionalna aktyvnist azotobakteru v gruntakh Lisostepu Ukrainy [Polyfunctional activity of azotobacter in Soils of the Forest-Steppe of Ukraine]. Ahroekolohichnyi zhurnal [Agroecological journal]. Spetsvypusk: 351–354. (In Ukr.).
Volkohon, V.V. (Ed), Nadkernychna, O.V., Tokmakova, L.M. еt al. (2010). Eksperymentalna gruntova mikrobiolohiia [Experimental soil microbiology]. Kyiv: Agrarian Science: 464. [In Ukr.].
Tkachyk, S.O. (Ed) (2014). Metodyka derzhavnoi naukovo-tekhnichnoi ekspertyzy sortiv roslyn. Metody vyznachennia pokaznykiv yakosti produktsii roslynnytstva [Methods of state scientific and technical examination of plant varieties. Methods for defining crop quality indicators]. Kyiv: 167. [In Ukr.].
Demyanyuk, O.S., Sherstoboeva, О.V., Bunas, A.A., Dmitrenko, O.V. (2018). Effects of different fertilizer systems and hydrothermal factors on microbial activity in the chernozem in Ukraine. Biosystems diversity. 26(4): 309–315.
Posypanov, G.S. (1985). Azotfiksatsiya bobovykh kultur v zavisimosti ot pochveno-klimaticheskikh rusloviy. Mineralnyy i biologicheskiy azot v zemledelii SSSR [Nitrogen fixation of legumes depending on soil and climatic channels. Mineral and biological nitrogen in the agriculture of the USSR]. Moskva: Nauka: 75–84. [In Russ.].
Kots, S.Ya. (2001). Rol biolohichnoho azotu u pidvyshchenni produktyvnosti silskohospodarskykh roslyn [The role of biological nitrogen in improving the productivity of agricultural plants]. Fiziologiya i biokhimiya kulturnykh rasteniy [Physiology and biochemistry of cultivated plants]. 33(3): 208–215. [In Ukr.].
Downloads
Published
Issue
Section
License
- The authors reserve the right to authorship their work and pass the journal the right to publish this work under a Creative Commons Attribution License license, which allows other persons to freely distribute the published work with the obligatory The authors of the original work and the first publication of this magazine.
- The authors have the right to make independent additional agreements on the nonexclusive dissemination of the work in the form in which it was published by this magazine (for example, to post work in the company's electronic storage or to publish as a monograph) , subject to the first publication of the link to this journal.
- Journal policy allows and encourages the placement of authors on the Internet (for example, in the repositories of institutions or on personal websites) manuscript work as to the presentation of this manuscript to the editorial board and during its editorial processing, as it contributes to The productive scientific discussion and positively affects the efficiency and dynamics of citation published work (see The Effect of Open Access).
