Genetic structure of the herds of two bighead carp species in «Аgrofirm Кolos» PLC
DOI:
https://doi.org/10.33730/2310-4678.3.2023.289322Keywords:
silver carp, bighead carp, alleles, genotype, heterozygosity, inbreedingAbstract
The allelic and genotypic composition of herds of silver carp and bighead carp of Agrofirma Kolos PLC (Kyiv region) was studied. The method of electrophoresis in a polyacrylamide gel with histochemical staining and subsequent genotyping of samples according to the three most variable blood proteins: transferrin, albumin, and esterase is used to detect molecular genetic markers. The number of three-year-old fish that were used to take blood samples for genetic research was 20 specimens for each species of fishes. The frequency of alleles and genotypes, actual and expected heterozygosity, and fixation indices were calculated. According to the results of the study, the predominance of heterozygous genotypes over homozygous genotypes at all three studied loci was found in the herds of both types of fish. The highest level of heterozygosity was established at the transferrin locus (98% in silver carp and 66.2% in bighead carp), the lowest was at the albumin locus (65% in silver carp and 50.8% in bighead carp). A slight excess of heterozygous individuals was found for transferrin and esterase loci in the herd of silver carp. For the bighead carp, this was found only in the transferrin locus. The influence of inbreeding on the formation of the genetic structure of herds of both species of fishes was revealed. To prevent the negative impact of inbreeding, it is recommended to use the high genetic variability of herds for the heterogeneous selection of fish for breeding purposes and to carry out cyclic genetic monitoring of the stock.
References
Alymov, S.I., Tretiak, O.M., Kovalenko, V.O., Prystaichuk, P.B. (2004). Pidvyshchennia efektyvnosti resursooshchadnykh tekhnolohii stavovoho rybnytstva v Ukraini [Increasing the efficiency of resource-saving technologies of pond fish farming in Ukraine]. Rybne hospodarstvo: mizhvidomch. tematych. nauk. zb. — nterdepartmental thematic scientific collection, 63, 3–6. K.: Ahrarna nauka [in Ukrainian].
Hrynzhevskyi, M.V., Sherman, I.M., Hrytsyniak, I.I. et al. (2006). Orhanizatsiia selektsiino-pleminnoi roboty v rybnytstvi [Organization of selection and breeding work in fish farming]. Kyiv: Rybka moia [in Ukrainian].
Kovalenko, V.O. (2005). Problemy ta perspektyvy rozvytku pleminnoi spravy u rybnytstvi Ukrainy [Problems and prospects for the development of tribal government in the Ukrainian region]. Rybne hospodarstvo: mizhvidomch. tematych. nauk. zb. — nterdepartmental thematic scientific collection, 64, 25–30. K.: Ahrarna nauka [in Ukrainian].
Chemerys, V.A., Dushka, V.I., Maksym, V.L. (2016). Stan ta perspektyvy rozvytku akvakultury v Ukraini [State and prospects of aquaculture development in Ukraine]. Naukovyi visnyk LNUVMBT imeni S.Z. Gzhytskoho — Scientific bulletin of Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, vol. 18, no. 2 (69), 169–175 [in Ukrainian].
Tarasiuk, S.I., Bekh, V.V., Nahorniuk, T.A., Rekrut, S.V. (2011). Provedennia henetychnoi ekspertyzy plidnykiv koropa: metod. rekomendatsii [Carrying out a genetic examination of carapaces: methodical recommendations]. K.: TsNTEI [in Ukrainian].
Zaloilo, O.V., Mariutsa, A.E., Tarasiuk, S.I. (2012). Osoblyvosti henetychnoi struktury populiatsii ukrainskykh ramchastykh koropiv Lymanskoho DVSRP Kharkivskoi oblasti [Peculiarities of the genetic structure of Ukrainian frame carp populations of the Liman SPAFE of the Kharkiv region]. Nauk.-tekhn. biul. In-tu biolohii tvaryn ta Derzh. n.-d. kontrol. in-tu vetpreparativ ta korm. dobavok. — Scientific and technical bulletin. Institute of Animal Biology and the State Scientific and Research Control Institute of Veterinary Preparations and Feed Additives, issue 13, no. 3/4, 289–293 [in Ukrainian].
Tarasiuk, S.I., Hrytsyniak, I.I. (2013). Molekuliarno-henetychni doslidzhennia v rybnytstvi [Molecular genetic research in medicine]. K.: Ahrarna nauka [in Ukrainian].
Nahorniuk, T.A. (2014). Osoblyvosti henetychnoi struktury riznovikovykh hrup biloho tovstolobyka [Features of the genetic structure of different age groups of white silver carp]. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu. Seriia “Tvarynnytstvo” — Bulletin of the Sumy National Agrarian University. Animal Husbandry Series, 7 (26), 51–54 [in Ukrainian].
Davis, B.J. (1964). Disc electrophoresis. II. Method and application to human serum proteins. Ann. N. Y. Acad. Sci., 121, 404–408 [in English].
Nei, M. (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89, 583–590 [in English].
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