Antagonistic activity of Bacillus bacteria isolated from the surface of mineral fertilizer granules

Authors

DOI:

https://doi.org/10.33730/2310-4678.1.2025.324367

Keywords:

antagonism, bacteria isolated from the surface of granules, Bacillus amyloliquefaciens, Bacillus cereus, phytopathogenic micromycetes

Abstract

The nature of the antagonistic interaction between the bacteria Bacillus amyloliquefaciens B-22, B. amyloliquefaciens subsp. plantarum 5/13 and B. cereus 3/7, previously isolated from the surface of mineral fertilizer granules, and phytopathogenic micromycetes was investigated, and the degree of inhibition of the development of fungal mycelium in laboratory conditions was established. The antagonistic properties of bacterial strains were determined by the method of double countercultures. The inhibitory activity of the microbial culture was estimated by the percentage of inhibition of the test culture when co-cultivated with antagonists. The strains B. amyloliquefaciens subsp. plantarum 5/13 and B. amyloliquefaciens B-22 had the greatest inhibitory effect against the causative agent of Alternaria blight. The growth of mycelium of F. culmorum 50716, F. moniliforme 156 and F. oxysporum 311 was most limited by the B. amyloliquefaciens B-22 strain (68.5%, 85.5% and 62.9% respectively). Among the bacterial cultures studied, strain 5/13 inhibited the development of F. solani EK 1 by 71.1%. Against the phytopathogen N. oryzae 5000, the studied strains B. amyloliquefaciens subsp. plantarum 5/13 and B. amyloliquefaciens B-22 showed a high level of inhibitory activity — 96.6 and 83.7%, respectively. Against the phytopathogen N. oryzae 3000, the inhibition rates were somewhat lower. B. cereus 3/7 slightly inhibited the growth of phytopathogenic fungi of the genus Nigrospora — 27.7% (N. oryzae 5000) and 31.6% (N. oryzae 3000). According to the results of the study of the antagonistic activity of the bacteria B. amyloliquefaciens B-22, B. amyloliquefaciens subsp. plantarum 5/13 and B. cereus 3/7, isolated from the surface of mineral fertilizer granules, it was found that strains B-22 and 3/7 exhibit a fairly high fungistatic effect on phytopathogenic test cultures. B. cereus 3/7 is the least effective in inhibiting the growth of phytopathogenic micromycetes.

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2025-02-24

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