The effects of gut indigenous microbiota on intensity of oxidative stress and the cytokine immunity in women with recurrent pyelonephritis.

Abstract

The aim of our study was to investigate the oxidative stress (OS) intensity and concentration of tumor necrosis factor alpha (TNF-α) and interleukin 10 (IL-10) depending on the content of Lactobacillus spp. in the colon of patients with recurrent pyelonephritis. Materials and methods. The observational study involved 64 women with recurrent pyelonephritis, aged 39.5±3.2 years. According to the quantitative content of Lactobacillus spp. in the patients’ intestine, the women were divided into two groups: the first group of the patients (n=38) had a deficit of Lactobacillus spp. in the intestine, and the second one (n=26) didn’t have any disorders. The intensity of OS was estimated by determining the OS index (OSI) as the ratio of total changes in the activity of oxidative processes to the total antioxidant capacity of blood. The blood concentration of TNF-α and interleukin 10 was determined. The local inflammation was characterized by the determination of the content of C-reactive protein (CRP), malondialdehyde (MDA) and the activity of N-acetyl-β-D-hexosaminidase (HEX) and β-galactosidase (β-gal) in urine. Results. The blood levels of OSI, MDA and TNF-α in the women with the deficit of Lactobacillus spp. in the gut were significantly higher compared with the deficit-free patients (р=0.03, р=0.01and р=0.007, respectively). Moreover, in the patients with the deficit of intestine lactobacillus spp., we observed high levels of CRP (р=0.045), HEX and β-gal (р=0.045) in the urine. In addition, a significant regression was found between IL-10 inthe blood and HEX in the urine (p=0.003), as well as MDA and TNF-α in the blood (p=0.02). Conclusions. Thus, the results of our work confirm the experimental studies data which demonstrate the leading role of gut indigenous microbiota in the development of the OS and inflammatory process.