The effects of gut indigenous microbiota on intensity of oxidative stress and the cytokine immunity in women with recurrent pyelonephritis.

Authors

  • N. M. Stepanova
  • V. E. Driyanska
  • L. V. Korol
  • L. Ya Mihal
  • V. S. Savchenko

DOI:

https://doi.org/10.26641/2307-0404.2018.1(part1).127251

Keywords:

lactobacilli, intestine, oxidative stress, cytokines, recurrent pyelonephritis

Abstract

The aim of our study was to investigate the oxidative stress (OS) intensity and concentration of tumor necrosis factor alpha (TNF-α) and interleukin 10 (IL-10) depending on the content of Lactobacillus spp. in the colon of patients with recurrent pyelonephritis. Materials and methods. The observational study involved 64 women with recurrent pyelonephritis, aged 39.5±3.2 years. According to the quantitative content of Lactobacillus spp. in the patients’ intestine, the women were divided into two groups: the first group of the patients (n=38) had a deficit of Lactobacillus spp. in the intestine, and the second one (n=26) didn’t have any disorders. The intensity of OS was estimated by determining the OS index (OSI) as the ratio of total changes in the activity of oxidative processes to the total antioxidant capacity of blood. The blood concentration of TNF-α and interleukin 10 was determined. The local inflammation was characterized by the determination of the content of C-reactive protein (CRP), malondialdehyde (MDA) and the activity of N-acetyl-β-D-hexosaminidase (HEX) and β-galactosidase (β-gal) in urine. Results. The blood levels of OSI, MDA and TNF-α in the women with the deficit of Lactobacillus spp. in the gut were significantly higher compared with the deficit-free patients (р=0.03, р=0.01and р=0.007, respectively). Moreover, in the patients with the deficit of intestine lactobacillus spp., we observed high levels of CRP (р=0.045), HEX and β-gal (р=0.045) in the urine. In addition, a significant regression was found between IL-10 inthe blood and HEX in the urine (p=0.003), as well as MDA and TNF-α in the blood (p=0.02). Conclusions. Thus, the results of our work confirm the experimental studies data which demonstrate the leading role of gut indigenous microbiota in the development of the OS and inflammatory process.

Author Biographies

N. M. Stepanova

SI «Institute of Nephrology NAMN of Ukraine»
Department of Nephrology and Dialysis

V. E. Driyanska

Laboratory of Immunology

L. V. Korol

Laboratory of Biochemistry 3
Degtyarivska str., 17-B, Kyiv, 04050, Ukraine

L. Ya Mihal

Laboratory of Biochemistry 3
Degtyarivska str., 17-B, Kyiv, 04050, Ukraine

V. S. Savchenko

Laboratory of Immunology

References

KolesnykMO, editor. [Fundamentals of Nephro­logy]. Kyiv. 2013;380. Ukrainian.

Lee BT, Ahmed FA, Lee Hamm L, et al. As­sociation of C-reactive protein, tumor necrosis factor-al­pha, and interleukin-6 with chronic kidney disease. BMC Nephrology. 2015;16:77. doi: 10.1186/s12882-015-0068-7

Circu ML, Aw TY. Intestinal redox biology and oxidative stress. Semin Cell Dev Biol. 2012;23:729-37. doi: 10.1016/j.semcdb.2012.03.014

Kamada N, Chen GY, Inohara N, Núñez G. Con­trol of Pathogens and Pathobionts by the Gut Microbiota. Nat Immunol. 2013;14(7):685-90. doi: 10.1038/ni.2608

Gaĭseniuk FZ, Driianskaia VE, Drannik GN. Pro­inflammatory cytokines in patients with pyelonephritis. Lik Sprava. 2013;6:32-7.

Grabe M, Bishop MC, Bjerklund-Johansen TE, et al. Guidelines on Urological Infections. European As­sociation of Urology; 2015. Available from: https://uro­web.org/wp-content/uploads/19-Urological-infections_LR2.pdf

Heymann F, Trautwein C, Tacke F. Monocytes and macrophages as cellular targets in liver fibrosis. Inflamm Allergy Drug Targets. 2009;8(4):307-18. doi: 10.2174/18715280978935223

Korol LV, Migal LYa, StepanovaNM. Intensity of oxidative stress and activity of angionetsin converting enzyme in blood of patients with uncomplicated pyelonephritis. Ukr. Biochem. J. 2017;89(2):99-105. doi: https://doi.org/10.15407/ubj89.02.099

Mardinoglu A, Shoaie S, Bergentall M, et al. The gut microbiota modulates host amino acid and glutathione metabolism in mice. Molecular Systems Biology. 2015;11(10). doi: 10.15252/msb.20156487

Mikkelsen KH, Frost M, Bahl MI. Effect of Antibiotics on Gut Microbiota, Gut Hormones and Glucose Metabolism. PLoS ONE. 2015;10(11). doi: 10.1371/journal.pone.0142352

Núria Macha, Dolors Fuster-Botellaa Endurance exercise and gut microbiota: A review. J. Sport and Health Science. 2016;6(2):179-192. https://https://doi.org/10.1016/j.jshs.2016.05.001" target="_blank">doi.org/10.1016/j.jshs.2016.05.001

Xu J, Xu C, Chen X, et al. Regulation of an antioxidant blend on intestinal redox status and major microbiota in early weaned piglets. Nutrition. 2014;30:584-9. doi: 10.1016/j.nut.2013.10.018

Jandhyala SM, Talukdar R, Subramanyam C, et al. Role of the normal gut microbiota World Journal of Gastroenterology: WJG. 2015;21(29):8787-803. doi: 10.3748/wjg.v21.i29.8787

Panda S, Elkhader I, Casellas F, et al. Short-term effect of antibiotics on human gut microbiota. PLoS One. 2014;9(4). doi: 10.1371/journal.pone.0095476

Puchades Montesa MJ, González Rico MA, et al. Solís Salguero MA. Study of oxidative stress in advanced kidney disease. Nefrología. 2009;29(5):464-73. doi: 10.5414/CN107639 PMID: 23782545

How to Cite

1.
Stepanova NM, Driyanska VE, Korol LV, Mihal LY, Savchenko VS. The effects of gut indigenous microbiota on intensity of oxidative stress and the cytokine immunity in women with recurrent pyelonephritis. Med. perspekt. [Internet]. 2018Apr.24 [cited 2024Jul.15];23(1(part1):129-35. Available from: https://journals.uran.ua/index.php/2307-0404/article/view/127251

Issue

Section

CLINICAL MEDICINE