State of collagenolysis in experimental periodontitis of bacterial-immune genesis and its correction with flavonol

A.Ye.  Demkovych; Yu.I.  Bondarenko; O.O.  Fastovets; A.O.  Hrad; P.A.  Hasiuk; O.V.  Denefil

doi:10.26641/2307-0404.2021.2.234488

Authors

A.Ye. Demkovych I. Horbachevsky Ternopil National Medical University, Department of Orthopedic Dentistry , Volia sq., 1, Ternopil, 46002, Ukraine, Ukraine https://orcid.org/0000-0001-9823-4283
Yu.I. Bondarenko I. Horbachevsky Ternopil National Medical University, Department of Pathological Physiology, Volia sq., 1, Ternopil, 46002, Ukraine, Ukraine https://orcid.org/0000-0003-2681-5526
O.O. Fastovets Dnipro State Medical University, Department of Orthopedic Dentistry, V. Vernadsky str., 9, Dnipro, 49044, Ukraine, Ukraine https://orcid.org/0000-0002-2769-3244
A.O. Hrad I. Horbachevsky Ternopil National Medical University, Department of Orthopedic Dentistry, Volia sq., 1, Ternopil, 46002, Ukraine, Ukraine https://orcid.org/0000-0001-5905-452X
P.A. Hasiuk I. Horbachevsky Ternopil National Medical University, Department of Orthopedic Dentistry, Volia sq., 1, Ternopil, 46002, Ukraine, Ukraine https://orcid.org/0000-0002-2915-0526
O.V. Denefil I. Horbachevsky Ternopil National Medical University, Department of Pathological Physiology, Volia sq., 1, Ternopil, 46002, Ukraine, Ukraine https://orcid.org/0000-0002-3606-5215

DOI:

https://doi.org/10.26641/2307-0404.2021.2.234488

Keywords:

periodontitis, inflammation, collagenolysis, oxyproline, quercetin

Abstract

The article presents an assessment of the dynamics of changes in the content of the marker of collagenolysis – free oxyproline in the homogeniate of soft tissues and bone in experimental bacterial-immune periodontitis and elucidation of the effect of flavonol quercetin on these indicators. The aim of this study was to determine the role of cytokinogenesis and the effect of flavonol on it in the pathogenesis, development and course of experimental periodontitis. During the experiment, a fragment of the mandible was taken from the animals, from which the soft tissues and bone were carefully separated. The state of collagen was determined by the content of free oxyproline in the soft and bone tissues. The concentration was determined according to the calibration graph and expressed in μmol/g. The results of studies of the indicators of the state of biopolymers of connective tissue structures of periodontium on the 7^th, 14^th and 30^th day of experimental bacterial-immune periodontitis and after its correction with flavonol (from the 7^th to the 14^th day of the experiment) are presented. The data on the nature of changes in the content of collagen monomers in the process of formation of the inflammatory focus in the periodontal complex are given. During the acute phase of the inflammatory process in rats there was revealed a slight increase in blood free oxyproline in bone homogenate and homogenate of soft periodontal tissues, on the 14^th day the dynamics continued to increase, at a later stage of the experiment, namely on the 30^th day, increase in bone resorption continued as compared to the 7^th and 14^th day. During the correction of disorders resulted from the development of this pathological process there was a decrease in the level of free oxyproline in the bone homogenate and homogenate of soft tissues of mandibular periodontium, as compared to the same indicators of animals who did not receive quercetin on the 14^th day. The use of flavonol quercetin, which, by affecting immune processes, limited the inflammatory response in periodontal tissues and stabilized collagenolysis processes in periodontal tissues was manifested by a decrease in free oxyproline in bone and soft tissue homogenates of experimental animals.

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