State of collagenolysis in experimental periodontitis of bacterial-immune genesis and its correction with flavonol

A.Ye.  Demkovych; Yu.I.  Bondarenko; O.O.  Fastovets; A.O.  Hrad; P.A.  Hasiuk; O.V.  Denefil

doi:10.26641/2307-0404.2021.2.234488

Автор(и)

A.Ye. Demkovych I. Horbachevsky Ternopil National Medical University, Department of Orthopedic Dentistry , Volia sq., 1, Ternopil, 46002, Ukraine, Україна https://orcid.org/0000-0001-9823-4283
Yu.I. Bondarenko I. Horbachevsky Ternopil National Medical University, Department of Pathological Physiology, Volia sq., 1, Ternopil, 46002, Ukraine, Україна https://orcid.org/0000-0003-2681-5526
O.O. Fastovets Dnipro State Medical University, Department of Orthopedic Dentistry, V. Vernadsky str., 9, Dnipro, 49044, Ukraine, Україна https://orcid.org/0000-0002-2769-3244
A.O. Hrad I. Horbachevsky Ternopil National Medical University, Department of Orthopedic Dentistry, Volia sq., 1, Ternopil, 46002, Ukraine, Україна https://orcid.org/0000-0001-5905-452X
P.A. Hasiuk I. Horbachevsky Ternopil National Medical University, Department of Orthopedic Dentistry, Volia sq., 1, Ternopil, 46002, Ukraine, Україна https://orcid.org/0000-0002-2915-0526
O.V. Denefil I. Horbachevsky Ternopil National Medical University, Department of Pathological Physiology, Volia sq., 1, Ternopil, 46002, Ukraine, Україна https://orcid.org/0000-0002-3606-5215

DOI:

https://doi.org/10.26641/2307-0404.2021.2.234488

Ключові слова:

periodontitis, inflammation, collagenolysis, oxyproline, quercetin

Анотація

The article presents an assessment of the dynamics of changes in the content of the marker of collagenolysis – free oxyproline in the homogeniate of soft tissues and bone in experimental bacterial-immune periodontitis and elucidation of the effect of flavonol quercetin on these indicators. The aim of this study was to determine the role of cytokinogenesis and the effect of flavonol on it in the pathogenesis, development and course of experimental periodontitis. During the experiment, a fragment of the mandible was taken from the animals, from which the soft tissues and bone were carefully separated. The state of collagen was determined by the content of free oxyproline in the soft and bone tissues. The concentration was determined according to the calibration graph and expressed in μmol/g. The results of studies of the indicators of the state of biopolymers of connective tissue structures of periodontium on the 7^th, 14^th and 30^th day of experimental bacterial-immune periodontitis and after its correction with flavonol (from the 7^th to the 14^th day of the experiment) are presented. The data on the nature of changes in the content of collagen monomers in the process of formation of the inflammatory focus in the periodontal complex are given. During the acute phase of the inflammatory process in rats there was revealed a slight increase in blood free oxyproline in bone homogenate and homogenate of soft periodontal tissues, on the 14^th day the dynamics continued to increase, at a later stage of the experiment, namely on the 30^th day, increase in bone resorption continued as compared to the 7^th and 14^th day. During the correction of disorders resulted from the development of this pathological process there was a decrease in the level of free oxyproline in the bone homogenate and homogenate of soft tissues of mandibular periodontium, as compared to the same indicators of animals who did not receive quercetin on the 14^th day. The use of flavonol quercetin, which, by affecting immune processes, limited the inflammatory response in periodontal tissues and stabilized collagenolysis processes in periodontal tissues was manifested by a decrease in free oxyproline in bone and soft tissue homogenates of experimental animals.

Посилання

Antomonov M. [Mathematical processing and analysis of medical and biological data]. Kyiv; 2017. p. 19. Russian. URL: http://www.health.gov.ua/www.nsf/16a436f1b0cca21ec22571b300253d46/522e94120f630ce5c225803b004b3867/$FILE/Antomonov_monogr_titul%2BOGLAVL%2Bvvedenie.pdf

Horoshko OM, Zakharchuk OI, Zamorsky II, Ezhned MA, Drachuk VM, Palamar AO, Sakhatska IM. [Influence of long-term administration of corvitin on proteolytic activity in rats with gentamicin nephropathy]. Ukrainskyi zhurnal medytsyny, biolohii ta sportu. 2019;4(1):41-45. Ukrainian. doi: https://doi.org/10.26693/jmbs04.01.041

Demkovych AYe. [Changes in lipoperoxidation parameters in experimental periodontitis of bacterial-immune genesis and the effect of quercetin on them]. Visnyk medychnykh i biolohichnykh doslidzhen. 2020;2:34-38. Ukrainian. doi: https://doi.org/10.11603/bmbr.2706-6290.2020.2.11263

Mazur IP, Slobodyannik MV. [Systemic antibacterial drugs in periodontology]. Sovremennaia stomatolohyia. 2016;1:32-38. Russian. Available from: http://nbuv.gov.ua/UJRN/ss_2016_1_10

Pysareva EV, Vlasov MYu, Holub YuV, Stadler ER. [Modification of the method for determination of oxyproline fractions in blood serum]. Vestn SamHU Estestvennonauchn ser. 2012;9(100):211-216. Russian. Available from: https://elib.grsu.by/doc/12044

Savelieva NN. [Phagocytic activity of blood neutrophils in patients with chronic generalized periodontitis of I-II severity on the background of parasitosis]. Immunopatolohiia, allerholohiia, infektol. 2017;2:45-50. Russian. doi: https://doi.org/10.14427/jipai.2017.2.45

Saturskaya AS, Usinsky RS, Saturskaya UV, Levchuk RD. [Dynamics of humoral immunity indicators at the stages of development of experimental diffuse cardiosclerosis depending on individual resistance of the organism to hypoxia]. Imunolohiia ta alerholohiia: nauka i praktyka. 2018;4:24-32. Russian.

Förster Y, Bernhardt R, Hintze V, Moller S, Schnabelrauch M, Scharnweber D, Rammelt S. Collagen/glycosaminoglycan coatings enhance new bone formation in a critical size bone defect – A pilot study in rats. Mater Sci Eng C Mater Biol Appl. 2017;71:84-92. doi: https://doi.org/ 10.1016/j.msec.2016.09.071

Demkovych A, Bondarenko Yu, Hasiuk P. Effects of quercetin on antioxidant potential in the experimental periodontitis development. Interv Med and App Sc. 2019;11(1):60-64. doi: https://doi.org/10.1556/1646.11.2019.06

Masola V, Zaza G, Arduini A, Onisto M, Gambaro G. Endothelial Glycocalyx as a Regulator of Fibrotic Processes. Int J Mol Sci. 2021;22(6):2996. Available from: https://www.mdpi.com/1422-0067/22/6/2996

Fukuhara D, Irie K, Uchida Y, Kataoka K, Akiyama K, Ekuni D, Tomofuji T, Morita M. Impact of commensal flora on periodontal immune response to lipopolysaccharid. Periodontol. 2018;89(10):1213-20. doi: https://doi.org/10.1002/jper.17-0567

Karthikeyan BV, Khanna D, Chowdhary KY, Prabhuji ML. The versatile subepithelial connective tissue graft: a literature update. Gen Dent. 2016;64(6):28-33. Available from: https://pubmed.ncbi.nlm.nih.gov/27814265/

Klingler CH. Glycosaminoglycans: how much do we know about their role in the bladder? Urologia. 2016;83(1):11-14.

doi: https://doi.org/ 10.5301/uro.5000184

Massi A, Bortolini O, Rango D, Bernardi T, Sacchetti G, Tacchini M, De Risi C. Research Progress in the Modification of Quercetin Leading to Anticancer Agents. Molecules. 2017;22(8):1270. doi: https://doi.org/10.3390/molecules22081270

Sculean A, Romanos G, Schwarz F, Ramanauskaite A, Keeve PL, Khoury F, Koo KT, Cosgarea R. Soft-Tissue Management as Part of the Surgical Treatment of Periimplantitis: A Narrative Review. Implant Dent. 2019;2:210-216. doi: https://doi.org/10.1097/id.0000000000000870