The mixed impact of chemical and physical factors on reproductive system of rats in conditions of cold stress.
DOI:
https://doi.org/10.26641/2307-0404.2016.4.90777Keywords:
reproductive system, nіtrobenzol, methyl tertiary butyl ether, electromagnetic radiation, cold stress, gonadotoxic action, mixed impactAbstract
The results of experimental investigations on studying features of gonadotoxic effect of nitrobenzene (NB), methyl tertiary butyl ether (MTBE) and electromagnetic radiation (EMR) both in conditions of cold stress and in conditions of thermal acceptability are given. The investigations were carried out on laboratory animals, in 30-fold intragastric administration of NB or MTBE in a dose of 1/10 LD50 and exposition of animals in two different thermal regimens – 4 hours a day, 5 times a week. Effect of EMR was simulated in 70 kHz frequency, 600 V/m voltage.The features of gonadotoxic effect of NB and MTBE were studied by the indices of spermatozoa functional state, general histological analysis of the state of testicle tissues, supplemented with morphometric values, was carried out,. The analysis of results of experimental research proves that morpho-functional changes of reproductive system in laboratory animals during the influence of chemical factors (NB and MTBE), physical factors (EMR) in two different thermal regimens are similar provided that there is the increase of gonadotoxic effect in mixed effect of mentioned factors and decreased temperature.
References
Sanotckii IV, Fomenko VN, Salnikova LS. [Met-hods of experimental studies to establish thresholds of action of industrial poisons on the generative function for the purpose of hygienic regulation: Guidance]. NII GT i PZ AMN SSSR, Moskva, 1978;35. Russian.
Nikolaev AA, Kuznetcova MG, Serdiukov VG. [Go¬nadotoxic action of millimeter radiation]. Astrakhan, AGMA. 2013;78. Russian.
Chorniy OP, Nikiforov VV, Rodkin DI, Nozhen¬ko VYu. [The current state of research on the influence of electromagnetic radiation on the human body]. Inzhenerni ta osvitni tehnologiyi v elektrotehnichnih i komp’yuternih sistemah. 2013;2(2):112-4. Russian.
Zolotaryova TA, Nasibullin BA, Yaroshenko NA, Zmievskiy AV. [Modern understanding of the mecha-nisms of stress caused by changes in the activity of spermatogenesis]. Mir meditsinyi i biologii. 2011;4:(7):134-6. Russian.
Yavorovskyi OP, Paustovskyi IuO. [Features of working conditions and health of workers employed in manufacturing and application of metyl tret-butyl ether refineries Ukraine]. Dovkillia ta zdorov’ia. 2008;3(46):60-63. Ukrainian.
European convention for the protection of verteb¬rate animals used for experimental and other scientific purpose: Council of Europe. Strasbourg, 1986;123:52.
Di Girolano F, Campanella L, Samperi R, Bachi A. [Mass spectrometric identification of hemoglobin modifications induced by nitrobenzene]. Ecotoxicology and Environmental Safety. 2009;72(5):1601-8.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2017 Medical Perspectives
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Submitting manuscript to the journal "Medicni perspektivi" the author(s) agree with transferring copyright from the author(s) to publisher (including photos, figures, tables, etc.) editor, reproducing materials of the manuscript in the journal, Internet, translation into other languages, export and import of the issue with the author’s article, spreading without limitation of their period of validity both on the territory of Ukraine and other countries. This and other mutual duties of the author and all co-authors separately and editorial board are secured by written agreement by special form to use the article, the sample of which is presented on the site.
Author signs a written agreement and sends it to Editorial Board simultaneously with submission of the manuscript.
