Electrophysiologic research of surgically restored median nerve (experimental study).

N. O. Borzykh; S. S. Strafun; S. I. Savosko

doi:10.26641/2307-0404.2018.1.124905

Автор(и)

N. O. Borzykh
S. S. Strafun
S. I. Savosko

DOI:

https://doi.org/10.26641/2307-0404.2018.1.124905

Ключові слова:

median nerve, injury, anastomosis

Анотація

In the experiment the possibility of providing effective regeneration of the injured median nerve in conditions of creation “protected” suture with intact ulnar nerve was studied. Experimental animals were divided into 4 groups: 1) complete neurotomy of the median nerve; 2) complete neurotomy of the median nerve with neurorrhaphy; 3) complete neurotomy of the median nerve and anastomosis creation between the median and the ulnar nerves in the distal site; 4) complete neurotomy of the median nerve and its neurorrhaphy with the anastomosis between the median and the ulnar nerves in the distal site. The histological study was carried out to identify the level of the nerve regeneration, state of the nerve distal segment and electrical conductivity of the surgically restored nerve on day 30 after the trauma. Morphometric and electrophysiological examinations showed that anastomosis creation allows to prevent atrophy and elimination of distal segment of median nerve and activate regenerative process in the injured nerve. The electrical conductivity of the nerve improved. An increased M-response of the forearm muscles was observed in groups with the anastomosis between the median and the ulnar nerves (the level of M-response recovery in the group 2 – 27,1%, group 3 – 18, 9%, group 4 – 84,6%). According to the study results we have made a conclusion that reinnervation of the muscles at an early stage of the median and the ulnar nerves regeneration was realized with the participation of high-threshold myelin nerve fibers.

Біографії авторів

N. O. Borzykh

SI “Institute of Traumatology and Orthopedics NAMS Ukraine” ^*
Bulvarno-Kudriavska str., 27, Kyiv, 01601, Ukraine

S. S. Strafun

SI “Institute of Traumatology and Orthopedics NAMS Ukraine” ^*
Bulvarno-Kudriavska str., 27, Kyiv, 01601, Ukraine

S. I. Savosko

Bogomolets National medical university ^**
Т. Shevchenko boul., 13, Kyiv, 01601, Ukraine

Посилання

Mikhaylyuk IG. [An anastomosis of Martin-Gruber and its clinical significance]. Клиницист. 2015;1:50-55. Russian.

Felippe MM,TellesFL, Soares ACL, et al. Anastomosis between median nerve and ulnar nerve in the forearm. J. Morphol. Sci. 2012;29(1):23-26.

Maznychenko AV, Pilyavskii AI, Vlasenko OV, Maisky VA. Fatigue of the dorsal neck muscles initiates c-fos expression in the rat spinal cord and hypothalamus. Neurophysiology. 2006;38(4):298-301.

Fernandez E, Lauretti L, Tufo T. M.D’Ercole End-to-side neurorrhaphy. Critical appraisal of experimental and clinical data. Acta Neurochirurg. Suppl. 2007;100(1):77-84.

TrindadeAB, Schestatsky P, Torres VF, et al. Functional and regenerative effects of local administration of autologous mononuclear bone marrow cells combined with silicone conduit on transected femoral nerve of rabbits. Res Vet Sci. 2015;102:27-33.

Kiechel S. Follow-up of peripheral nerve lesions. In: High-Resolution Sonography of the Peripheral Nervous System. Springer, 2003;132.

Kimura J. Electrodiagnosis in diseases of nerve and muscle: principles and practice. 3rd ed.OxfordUniversityPress; 2001.

Kostomitsopoulos NG, Durasevic SF. The ethical justification for the use of animals in biomedical research. Arch. Biol. Sci.,Belgrade. 2010;62(3):781-7.

Nadire Unver Dogan, Ismihan Ilknur Uysal, Muzaffer Seker. The communications between the ulnar and median nerves in upper limb. Neuroanatomy. 2009;8:15-19.

Namgung U. The role of Schwann cell-axon interaction in peripheral nerve regeneration. Cells Tissues Organs. 2014;200(1):6-12.

Bulut T, Akgün U, Çıtlak A, et al. Prognostic factors in sensory recovery after digital nerve repair. ActaOrthopTraumatolTurc. 2016;50(2):157-61.