Protective effect of germanium citrate in endotoxin-induced ovarian dysfunction in mice.
DOI:
https://doi.org/10.26641/2307-0404.2018.1(part1).127240Ключові слова:
germanium citrate, lipopolysaccharide, oocytes, granulosa cells, DNA damage, apoptosis, necrosisАнотація
Now it is known that endotoxin of gram-negative bacteria – lipopolysaccharide (LPS) is associated with disturbances of reproductive function in women. To simulate an ovarian dysfunction in endotoxemia and to explore a possible protective action of germanium (Ge) citrate obtained via nanotechnology we used LPS administration into female mice (3 mg/kg). Treatment with LPS caused pathological changes in mouse ovaries at 24h post-injection: an impairment of oocyte maturation in vitro, DNA damage in granulosa cells (as estimated by DNA-comet assay) and a decrease in their viability by increasing both necrosis and apoptosis. Pretreatment of mice with Ge citrate (i.p. 100 mkg/kg, 2 injections: 24 h and 1 h before LPS) was effective to reduce genotoxic stress and cell death. Ge citrate administration to LPS-treated mice significantly enhanced oocyte quality (as indicated by improvement in oocyte maturation) that may be due to cytoprotective effect of this compound on surrounding follicular cells. Our results support potential therapeutic application of Ge citrate to improve reproductive function in endotoxemia.
Посилання
Fedoruk RS, Tesarivska UI, Khrabko MI, Tsap MM, Dolaychuk OP, Kropyvka SI. [Haematological and biochemical parameters of the F2 rats organism in a period of prolonged watering of nano-Ge citrate]. Bìol. Tvarin. 2017;19(3):115–21. Ukrainian. doi: http://dx.doi.org/10.15407/animbiol19.03.115
Grushka NG. [The effect of poly(ADP-ribose) polymerase inhibitor 4-hydroxyquinazoline on death of immune cells under immune complex-mediated injury in mice]. Fiziol. Zh. 2017;63(1):43-50. Ukrainian. doi: https://doi.org/10.15407/fz63.01.043
Kosinov MV, Kaplunenko VH. [Pat. ofUkraineN 38391. 2009. IPC (2006): C07C 51/41, C07F 5/00, C07F 15/00, C07C 53/126 (2008.01), C07C 53/10 (2008.01), A23L 1/00, B82B 3/00. Method for metal carboxylates obtaining "Nanotechnology of obtaining metal carboxylates"]. Publ. 12.01.2009. Ukrainian.
Fedoruk RS, Khrabko MI, Tsap MM, Martsynko OE. [Growth, development and reproductive function of female rats and their offspring viability at the conditions of the watering of different doses of citrate germanium]. Bìol.Tvarin. 2016;18(3):97-106. Ukrainian. doi: http://dx.doi.org/10.15407/animbiol18.03.097
Sribna V, Grushka N, Yanchiy R. [DNA single-strand breaks of follicular cells surrounding the oocyte, thymic and lymph nodes cells under the conditions of experimental immune complex-mediated inflammation and antioxidant treatment]. Visnyk Probl Biol Med. 2016; 2(3):195-99. Ukrainian.
Enukidze G, Anikhovskaya I, Marachev A, Yakovlev M. [Endotoxin aggression in the pathogenesis of chronic inflammatory diseases of small pelvis organs and infertility, or an antiendotoxin approach to their treatment]. Fiziol Cheloveka. 2006;32(3):117-23. Russian. doi: 10.1134/S0362119706030169
Li L, Ruan T, Lyu Y, Wu B. Advances in Effect of Germanium or Germanium Compounds on Animals – A Review. Journal of Biosciences and Medicines. 2017;5:56-73. doi: 10.4236/jbm.2017.57006
Spivak NYa, Shepel EA,ZholobakNM, Shcherbakov AB, et al. Ceria nanoparticles boost activity of aged murine oocytes. Nano Biomed.Eng.2012;4(4), 188-94. doi: 10.5101/nbe.v4i4.p188-194
CollinsAR.The comet assay for DNA damage and repair: principles, applications, and limitations. Mol Biotechnol. 2004;26(3):249-61. doi: 10.1385/MB:26:3:249
Dolaychuk OP, Fedoruk RS, Kropyvka SJ. Physiological Reactivity and Antioxidant Defense System of the Animal Organism Induced by Germanium, Chromium, and Selenium "Nanoaquacitrates". Agricultural science and practice. 2015;2:50-55. doi: 10.15407/agrisp2.02.050
Shepel E, Grushka N, Makogon N, Voznesenskaya T, Yanchii R. Inhibition of poly (ADP-ribose) polymerase (PARP) protects against experimental immune complex-induced ovarian failure in mice. Int J Health Sci Res. 2016;6(11):103-8.
Tremellen K, Syedi N, Tan S, Pearce K. Metabolic endotoxaemia-a potential novel link between ovarian inflammation and impaired progesterone production. Gynecol Endocrinol. 2015;31(4):309-12. doi: 10.3109/09513590.2014.994602
Nakamura T, Takeda T, Tokuji Y. The Oral Intake of Organic Germanium, Ge-132, Elevates α-Tocopherol Levels in the Plasma and Modulates Hepatic Gene Expression Profiles to Promote Immune Activation in Mice. Int J Vitam Nutr Res. 2014;84(3-4):183-95. doi: 10.1024/0300-9831/a000205
Makogon N, Voznesenskaya Т, Bryzgina Т, Sukhina V, Grushka N, Аlexeyeva І. Poly (ADP-ribose) polymerase inhibitor, 3-aminobenzamide, protects against experimental immune ovarian failure in mice. Reprod Biol. 2010;10(3):215-26.
Shimizu T. Molecular and cellular mechanisms for the regulation of ovarian follicular function in cows. J Reprod Dev. 2016;62(4):323-9. doi: 10.1262/jrd.2016-044.
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