Results of biochemical and histological studies after restoration of bone defects using cellular technologies in dental patients

А.V. Bambuliak; N.B. Kuzniak; L.Y. Lopushniak; І.І. Dronyk; О.О.  Myhal

doi:10.26641/2307-0404.2024.2.307615

Автор(и)

А.V. Bambuliak Bukovinian State Medical University, Teatralna sq., 2, Chernivtsi, 58002, Україна https://orcid.org/0000-0002-6383-9327
N.B. Kuzniak Bukovinian State Medical University, Teatralna sq., 2, Chernivtsi, 58002, Україна https://orcid.org/0000-0002-4020-7597
L.Y. Lopushniak Bukovinian State Medical University, Teatralna sq., 2, Chernivtsi, 58002, Україна https://orcid.org/0000-0001-8362-406X
І.І. Dronyk Bukovinian State Medical University, Teatralna sq., 2, Chernivtsi, 58002, Україна https://orcid.org/0000-0001-8382-4379
О.О. Myhal Danylo Halytsky Lviv National Medical University, Pekarska str., 69, Lviv, 79010, Україна https://orcid.org/0000-0001-8000-8827

DOI:

https://doi.org/10.26641/2307-0404.2024.2.307615

Ключові слова:

multipotent mesenchymal stromal cells, hydroxyapatite, bone defect, patient, dentistry

Анотація

Objective of the research was to find out the effectiveness of the use of osteoplastic materials based on the multipotent mesenchymal stromal cells of the adipose tissue during surgical treatment for restoration of bone defects in dental patients. The clinical material included examination of 79 patients who underwent replacement of bone defects of the alveolar processes and/or an alveolar part of the appropriate jaw by means of different osteoplastic materials and their combinations or without their application (the control group). Acid phosphatase activity in the oral fluid of patients was determined by means of the unified “end point” method. Alkaline phosphatase activity in the oral fluid was determined using a set of reagents produced by the company “Filisit-Diagnostika” (Ukraine). Oral fluid mineralization index (MI) of patients was calculated according to the ratio of enzymatic activity of alkaline phosphatase (ALP) to acid phosphatase (AP) according to the formula: MI=ALP/AP. Histological material was taken at the stage of installation of a dental implant. It was established that the use of a composition based on multipotent mesenchymal stromal cells of adipose tissue, platelet-enriched blood plasma and bone tissue substitute containing hydroxyapatite contributes to the improvement of regenerative processes in the bone tissue of the jaws, which is confirmed by a 28.68% decrease in acid phosphatase activity, an increase by 25, 97% of alkaline phosphatase activity in the oral fluid of group B patients and, accordingly, a 77.08% increase in the ratio of alkaline to acid phosphatase. According to the results of histological studies and phase-contrast microscopy, after 6-8 months of observation, in patients of group B, the areas of jaw defects were filled with newly formed bone tissue, identical to native bone, and the resulting trepanobioptas had the structure of cancellous bone with osteoblastic elements forming a chain around osteoid tissue. Having analyzed the results of biochemical and histological studies it was established that application of an osteoplastic composition based on the multipotent mesenchymal stromal cells of the adipose tissue, bone tissue substitute with hydroxyapatite and platelet-rich plasma allows restoration of bone defects of the alveolar processes and an alveolar part of the appropriate jaw in shorter terms. The method is an optimal alternative to traditional osteoplastic materials to be used in modern dental practice and dental implantation.

Посилання

Kwon SG, Kwon YW, Lee TW, Park GT, Kim JH. Recent advances in stem cell therapeutics and tissue engineering strategies. Biomater Res. 2018 Dec 19;22:36. doi: https://doi.org/10.1186/s40824-018-0148-4

Bambuliak A, Kuzniak N, Honcharenko V, Ostafiychuk M, Palamar A. Osteoplastic properties of multipo¬tent mesenchymal stromal cells of adipose tissue. Wiad Lek. 2021;74(10 pt 1):2374-8. doi: https://doi.org/10.36740/WLek202110103

Gui C, Parson J, Meyer GA. Harnessing adipose stem cell diversity in regenerative medicine. APL Bioeng. 2021 Apr 1;5(2):021501. doi: https://doi.org/10.1063/5.0038101

Li Y, Jin D, Xie W, Wen L, Chen W, Xu J, et al. PPAR-γ and Wnt Regulate the Differentiation of MSCs into Adipocytes and Osteoblasts Respectively. Curr Stem Cell Res Ther. 2018 Feb 23;13(3):185-92. doi: https://doi.org/10.2174/1574888X12666171012141908

Belluci MM, de Molon RS, Rossa C Jr, Tetradis S, Giro G, Cerri PS, et al. Severe magnesium deficiency compromises systemic bone mineral density and aggravates inflammatory bone resorption. J Nutr Biochem. 2020 Mar;77:108301. doi: https://doi.org/10.1016/j.jnutbio.2019.108301

Uchida Y, Irie K, Fukuhara D, Kataoka K, Hattori T, Ono M, et al. Commensal Microbiota Enhance Both Osteoclast and Osteoblast Activities. Molecules. 2018;23(7):1517. doi: https://doi.org/10.3390/molecules23071517

Ge W, Jiang J, Baroja ML, Arp J, Zassoko R, Liu W, et al. Infusion of mesenchymal stem cells and rapamycin synergize to attenuate alloimmune responses and promote cardiac allograft tolerance. American Journal of Transplantation. 2019;9:1760-72. doi: https://doi.org/10.1111/j.1600-6143.2009.02721

Graziani F, Chappuis V, Molina A, Lazarin R, Schmid E, Chen S, et al. Effectiveness and clinical perfor-mance of early implant placement for the replacement of single teeth in anterior areas: A systematic review. J Clin Periodontol. 2019 Jun;46(Suppl 21):242-56. doi: https://doi.org/10.1111/jcpe.13092

Al-Obaidi MM, Al-Bayaty FH, Al Batran R, Ibrahim OE, Daher AM. Ellagic Acid Increases Osteocalcin and Alkaline Phosphatase After Tooth Extraction in Nicotinic-Treated Rats. Curr Pharm Des. 2016;22(16):2403-10. doi: https://doi.org/10.2174/138161282216160428002842

Skrypa OL, Bandrivsky YL. Determining the frequency of functional disorders of the TMJ in patients with mandible fractures depending on the location. Wiadomości Lekarskie. 2020;73(2):245-9. doi: https://doi.org/10.36740/WLek202002107

Chen Q, Cai J, Li X, Song A, Guo H, Sun Q, et al. Progranulin Promotes Regeneration of Inflammatory Periodontal Bone Defect in Rats via Anti-inflammation, Osteoclastogenic Inhibition, and Osteogenic Promotion. Inflammation. 2019 Feb;42(1):221-34. doi: https://doi.org/10.1007/s10753-018-0886-4

Pilawski I, Tulu US, Ticha P, Schüpbach P, Traxler H, Xu Q, et al. Interspecies Comparison of Alveolar Bone Biology, Part I: Morphology and Physiology of Pristine Bone. JDR Clin Trans Res. 2020:2380084420936979. doi: https://doi.org/10.1177/2380084420936979

Bandrivsky YL, Bandrivska OO, Shkrebnyuk RY, Dyryk VT. Prevalence of the generalized periodontitis in patients with different groups blood in depending on age and periodontal biotype. Wiad Lek. 2020;73(1):119-22. doi: https://doi.org/10.36740/WLek202001123

Meza-Zepeda LA, Noer A, Dahl JA, Micci F, Myklebost O, Collas P. High-resolution analysis of genetic stability of human adipose tissue stem cells cultured to senescence. J Cell Mol Med. 2018 Apr;12(2):553-63. doi: https://doi.org/10.1111/j.1582-4934.2007.00146x

Bambuliak АV. [Substantiation efficiency of the surgical treatment of jaw bones defects with the use of cell technology]. [dissertation]. Chernivtsi: Bukovinian State Medical University; 2023. 417 р. Ukrainian.

Bandrivsky Y, Bandrivska O, Gnid R, Minko L, Shevchuk M. Indicators of markers of bone metabolism in patients with generalized periodontitis depending on blood group. Arch Balk Med Union. 2019;54(1):72-7. doi: https://doi.org/10.31688/ABMU.2019.54.1.10

Hruzieva TS, Lekhan VM, Ohniev VA, Haliienko LI, Kriachkova LV, Palamar BI, et al. [Biostatistics]. Vinnytsia: New Book; 2020. 384 p. Ukrainian.

Storti G, Scioli MG, Kim BS, Cervelli V. Adiposederived stem cells in bone tissue engineering: useful tools with new application. Stem Cells Int. 2019;2019:3673857. doi: https://doi.org/10.1155/2019/3673857

Wankhade UD, Shen M, Kolhe R, Fulzele S. Advances in adipose-derived stem cell isolation, characte-rization, and application in regenerative tissue engineering. Stem Cells Int. 2016;2016:3206807. doi: https://doi.org/10.1155/2016/3206807