Oral microbiocenosis state under different approaches of replacing dental crown defects

Автор(и)

DOI:

https://doi.org/10.26641/2307-0404.2024.3.313603

Ключові слова:

bacteria, fungi, microbiological study, microbiota, microbiocenosis, resistance, biofilms, adhesiveness

Анотація

Dental caries is one of the most common health problems. The aim of the research was to study the spectrum of microbiota, which is part of the dental biofilm, and its biological properties. The study involved 90 participants of the study group and 20 of the control group. Dental plaque and saliva were used as principal clinical specimens. The main research method was bacteriological. All samples from study participants were positive for microbiota. At the initial collection of material, 395 unique isolates were obtained: 338 from patients with caries and 57 from healthy volunteers. The microbiocenosis of the oral cavity associated with the development of caries contained such eudodominants as Streptococcus mutans, Peptostreptococcus spp., Fusobacterium spp., Prevotella spp. and dominants Streptococcus pyogenes, Enterobacterales, Prevotella spp. and Candida albicans. In contrast the eudominants of the healthy oral cavity were microorganisms with known commensal properties, in particular Streptococcus salivarius, Aerococcus viridans, Veillonella spp. After the dental treatment, significant shifts in the taxonomic composition of the dental biofilm were observed. Therefore, the composition of the oral cavity microbiocenosis in participants who underwent restoration of the dental crown defects with zirconium inserts approached patients to the healthy cohort participants. The eudodominants of the oral microbiocenosis of the participants who underwent direct restoration were S. mitis, Peptostreptococcus spp. and Veillonella spp. In zirconium restoration, the commensals S. salivarius and A. viridans were eudodominants. The obtained isolates of opportunists possessed high virulence properties. Streptococcus spp. cultures were sensitive to norfloxacin screening in only 28.6% of cases. Among cultures of the genus Staphylococcus, 23.8% were methicillin resistant. Representatives of Enterobacterales had chemotherapeutic sensitivity that varied significantly depending on the group of antimicrobial agents. Using disks with phenylboronic acid, ethylenediaminetetraacetic acid and cloxacillin, it was established that Klebsiella spp. were producers of class A carbapenemases (n=3) and contained AmpC (n=3). Carbapenemases were also produced by at least 65.2% of the studied cultures of non-fermenting gram-negative microorganisms. In our study, all obtained isolates were also endowed with the ability to form a biofilm. Therefore, microbiological research in dentistry should be considered as one of the objective methods for assessment of health of the oral cavity and will also have advantages in choosing a method of correcting defects of dental crown parts and in some cases, making an informed decision about the prescription of antimicrobial treatment.

Посилання

Giacaman RA, Fernández CE, Muñoz-Sandoval C, León S, García-Manríquez N, Echeverría C, et al. Understanding dental caries as a non-communicable and behavioral disease: Management implications. Front Oral Health. 2022;3:64479. doi: https://doi.org/10.3389/froh.2022.764479

Thanetchaloempong W, Koontongkaew S, Utispan K. Fixed Orthodontic Treatment Increases Carioge-nicity and Virulence Gene Expression in Dental Biofilm. J Clin Med. 2022;11(19):5860. doi: https://doi.org/10.3390/jcm11195860

Spatafora G, Li Y, He X, Cowan A, Tanner ACR. The Evolving Microbiome of Dental Caries. Micro-organisms. 2024;12(1):121. doi: https://doi.org/10.3390/microorganisms12010121

Trottini M, Campus G, Corridore D, Cocco F, Cagetti MG, Vigo MI, et al. Assessing the Predictive Performance of Probabilistic Caries Risk Assessment Models: The Importance of Calibration. Caries Res. 2020;54(3):258-65. doi: https://doi.org/10.1159/000507276

Carroll CK, Pfaller AM. Manual of Clinical Microbiology, 4 Volume Set. 13-st ed. ASM Books; 2023. 3456 p.

Efimenko AO, Ishchenko OV. [Features of microbiological research in dental practice]. Perspektyvy ta innovatsiyi nauky. 2023;14(32):969-86. Ukrainian. doi: https://doi.org/10.52058/2786-4952-2023-14(32)-969-986

The European Committee on Antimicrobial Susceptibility Testing. Breakpoint tables for interpretation of MICs and zone diameters. Version 13.0 [Internet]. 2023 [cited 2024 Mar 15]. Available from: http://www.eucast.org

Ishchenko OV, Plotnikova MM, Stolyetova YuYu, Sharun OV, Stepanskyi DO. [The influence of different concentrations of pectin on the adhesive properties of clinical isolates obtained from patients with cystic fibrosis.] Vistnyk problem biolohiyi i medytsyny. 2020;1(155):137-40. Ukrainian. doi: https://doi.org/10.29254/2077-4214-2020-1-155-137-140

Toc DA, Csapai A, Popa F, Popa C, Pascalau V, et al. Easy and Affordable: A New Method for the Studying of Bacterial Biofilm Formation. Cells. 2022;11(24):4119. doi: https://doi.org/10.3390/cells11244119

Ryzhov OA, Penkin YuM. [Statistical methods of processing the results of medical and biological research]. Magnolia; 2022. 160 p. Ukrainian.

Zhang L, Chen X, Ren B, Zhou X, Cheng L. Helicobacter pylori in the Oral Cavity: Current Evidence and Potential Survival Strategies. Int J Mol Sci. 2022 Nov 7;23(21):13646. doi: https://doi.org/10.3390/ijms232113646

Zhu Y, Wang Y, Zhang S, Li J, Li X, Ying, et al. Association of polymicrobial interactions with dental caries development and prevention. Front Microbiol. 2023;14:1237596. doi: https://doi.org/10.3389/fmicb.2023.1237596

Lei L, Long L, Yang X, Qiu Y, Zeng Y, Hu T, et al. The VicRK Two-component system regulates Strepto-coccus mutans virulence. Curr Issues Mol Biol. 2019;32:167-200. doi: https://doi.org/10.21775/cimb.032.167

Acet Ö, Erdönmez D, Acet B, Odabaşı M. Nacyl homoserine lactone molecules assisted quorum sensing: Effects consequences and monitoring of bacteria talking in real life. Arch Microbiol. 2021;203:3739-49. doi: https://doi.org/10.1007/s00203-021-02381-9

Lu Y, Lin Y, Li M, He J. Roles of Streptococcus mutans-Candida albicans interaction in early childhood caries: a literature review. Front Cell Infect Microbiol. 2023 May 16;13:1151532. doi: https://doi.org/10.3389/fcimb.2023.1151532

Li Y, Huang S, Du J, Wu M, Huang X. Current and prospective therapeutic strategies: tackling Candida albicans and Streptococcus mutans cross-kingdom biofilm. Front Cell Infect Microbiol. 2023;13:1106231. doi: https://doi.org/10.3389/fcimb.2023.1106231

Kim HE, Liu Y, Dhall A, Bawazir M, Koo H, Hwang G. Synergism of Streptococcus mutans and Can-dida albicans Reinforces Biofilm Maturation and Acidogenicity in Saliva: An In Vitro Study. Front Cell Infect Microbiol. 2021 Feb 19;10:623980. doi: https://doi.org/10.3389/fcimb.2020.623980

Contaldo M, Lucchese A, Lajolo C, Rupe C, Di Stasio D, Romano A, et al. The Oral Microbiota Changes in Orthodontic Patients and Effects on Oral Health: An Overview. J Clin Med. 2021;10(4):780. doi: https://doi.org/10.3390/jcm10040780

Ionescu AC, Hahnel S, Delvecchio P, Ilie N, Moldovan M, Zambelli V, et al. Microbiological models for accelerated development of secondary caries in vitro. J Dent. 2022;127:104333. doi: https://doi.org/10.1016/j.jdent.2022.104333

Bollen C, Hakobayan G, Jörgens M. Onepiece versus two-piece ceramic dental implants. Br Dent J. 2024;236(5):383-7. doi: https://doi.org/10.1038/s41415-024-7123-3

D’Ambrosio F, Santella B, Di Palo MP, Giorda-no F, Lo Giudice R. Characterization of the Oral Mic-robiome in Wearers of Fixed and Removable Implant or Non-Implant-Supported Prostheses in Healthy and Pathological Oral Conditions: A Narrative Review. Microorganisms. 2023;11(4):1041. doi: https://doi.org/10.3390/microorganisms11041041

CDC. Antibiotic Resistance Threats in the United States, 2019. Atlanta, GA: U.S. Department of Health and Human Services, CDC [Internet]. 2019 [cited 2024 Mar 15]. doi: http://dx.doi.org/10.15620/cdc:82532

Iskandar K, Molinier L, Hallit S, Sartelli M, Hardcastle TC, Haque M, et al. Surveillance of antimicrobial resistance in low- and middle-income countries: a scattered picture. Antimicrob Resist Infect Control. 2021;10(1):63. doi: https://doi.org/10.1186/s13756-021-00931-w

Kiros A, Saravanan M, Niguse S, Gebregziabher D, Kahsay G, Dhandapani R, et al. Bacterial Profile, Antimicrobial Susceptibility Pattern, and Associated Factors among Dental Caries-Suspected Patients Attending the Ayder Comprehensive Specialized Hospital and Private Dental Clinic in Mekelle, Northern Ethiopia. Biomed Res Int. 2022;2022:3463472. doi: https://doi.org/10.1155/2022/3463472

Philips OO, Timothy O, Charlse II, Osamuyimen I. Susceptibility Pattern of Bacterial Isolates from Dental Caries Patients Attending Clinic at Irrua Specialist Teaching Hospital, Irrua, Nigeria. J Biomed Res Environ Sci. 2021;2(9):784-9. doi: https://doi.org/10.37871/jbres1311

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Опубліковано

2024-10-16

Як цитувати

1.
Sardykov Y, Ishchenko O, Fastovets O. Oral microbiocenosis state under different approaches of replacing dental crown defects. Med. perspekt. [інтернет]. 16, Жовтень 2024 [цит. за 24, Листопад 2024];29(3):151-6. доступний у: https://journals.uran.ua/index.php/2307-0404/article/view/313603

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