Increased secretion of irisin hormone during physical exercise: a systematic review

N.S.R. Pembayun; J.C. Wibawa; J. Lobo; M.A.A. Ardha; Nurhasan Nurhasan; S.C.Y. Hartati; A. Komaini; N. Ayubi

doi:10.26641/2307-0404.2025.4.348350

Authors

N.S.R. Pembayun Universitas Negeri Surabaya, Surabaya, Jawa Timur, 60213, Indonesia https://orcid.org/0000-0001-6816-8705
J.C. Wibawa STKIP PGRI Trenggalek, Supriyadi str., 22, Trenggalek, Jawa Timur, 66319, Indonesia https://orcid.org/0000-0002-4667-5981
J. Lobo Bulacan State University, Malolos, Bulacan, 3000, Philippines https://orcid.org/0000-0002-2553-467X
M.A.A. Ardha Universitas Negeri Surabaya, Surabaya, Jawa Timur, 60213, Indonesia https://orcid.org/0000-0002-9192-2072
Nurhasan Nurhasan Universitas Negeri Surabaya,Surabaya,Jawa Timur,60213, Indonesia https://orcid.org/0000-0003-2790-5777
S.C.Y. Hartati Universitas Negeri Surabaya, Surabaya, Jawa Timur, 60213, Indonesia http://orcid.org/0009-0004-5698-9185
A. Komaini Universitas Negeri Padang, Prof. Dr. Hamka str., kec. Padang Utara, Padang, Sumatera Barat, 25171, Indonesia https://orcid.org/0000-0002-2955-0175
N. Ayubi Universitas Negeri Surabaya, Surabaya, Jawa Timur, 60213, Indonesia https://orcid.org/0000-0002-5196-6636

DOI:

https://doi.org/10.26641/2307-0404.2025.4.348350

Keywords:

physical exercise, irisin, health, obesity

Abstract

Obesity is a medical condition characterized by the accumulation of excess fat in the body, which can increase the risk of various chronic diseases such as type 2 diabetes, hypertension, and heart disease. Obesity also correlates with an increase in metabolic syndrome diseases that have a negative impact on human health. The prevalence of obesity continues to increase globally, including in developing countries, along with changes in diet and lifestyle. Genetic, environmental, and behavioral factors play an important role in the development of obesity. The impact is not only limited to physical health, but also includes psychological and social aspects. Therefore, it is important to immediately address this problem. Irisin is one of the hormones that plays a major role in reducing obesity rates. The purpose of this study was to determine how physical exercise can increase the expression of the irisin hormone. For this study, a number of journal databases were searched, such as Pubmed, Embase, Science Direct, Web of Science, and Scopus. This study considered various variables, including research on physical activity and the irisin hormone published in journals in the last five years. The analysis included only publications from reputable scientific journals that met the established inclusion criteria, namely: the studies had to involve human participants, and the interventions had to consist of physical exercise. Using databases from Pubmed, Science Direct, Embase, Web of Science, and Scopus, 104 publications were found. Approximately ten carefully selected publications discussed the need for a systematic review analysis. The standard operating procedure for the investigation was developed using – preferred reporting items for systematic reviews and meta-analyses (PRISMA). The results of the systematic review showed an increase in irisin expression during physical activity. The limitation of this review only concerned the effect of physical activity on the irisin hormone. The best dose given during physical exercise to increase irisin expression is still not fully understood, therefore it is important to conduct further studies that discuss an adequate and optimal dose of physical activity to increase irisin.

References

WHO. Who European Regional Obesity Report 2022 [Internet]. 2022 [cited 2025 Aug 12]. Available from: http://apps.who.int/bookorders

Clemente-Suárez VJ, et al. The Role of Adipokines in Health and Disease. Biomedicines. 2023;11(5):1290. doi: https://doi.org/10.3390/biomedicines11051290

Awaluddin SM, Lim KK, Shawaluddin NS. Global prevalence of overweight and obesity among healthcare workers: A systematic review protocol. JBI Evid Synth. 2024 Nov 1;22(11):2342-9. doi: https://doi.org/10.11124/JBIES-23-00454

Muzurović EM, Volčanšek Š, Tomšić KZ, Janež A, Mikhailidis DP, Rizzo M, et al. Glucagon-Like Peptide-1 Receptor Agonists and Dual Glucose-Dependent Insulino¬tropic Polypeptide/Glucagon-Like Peptide-1 Receptor Agonists in the Treatment of Obesity/Metabolic Syndrome, Prediabetes/Diabetes and Non-Alcoholic Fatty Liver Disease – Current Evidence. J Cardiovasc Pharmacol Ther. 2022 Jan-Dec;27:10742484221146371. doi: https://doi.org/10.1177/10742484221146371

Park S, Lee S, Kim Y, Lee Y, Kang MW, Han K, et al. Altered risk for cardiovascular events with changes in the metabolic syndrome status a nationwide population-based study of approximately 10 million persons. Ann Intern Med. 2019 Dec 17;171(12):875-84. doi: https://doi.org/10.7326/M19-0563

Kazi DS, Elkind MSV, Deutsch A, Dowd WN, Heidenreich P, Khavjou O, et al. Forecasting the Economic Burden of Cardiovascular Disease and Stroke in the United States Through 2050: A Presidential Advisory From the American Heart Association. Circulation. 2024 Jul 23;150(4):e89-e101. doi: https://doi.org/10.1161/CIR.0000000000001258

Chooi YC, Ding C, Magkos F. The epidemiology of obesity. Metabolism. 2019 Mar;92:6-10. doi: https://doi.org/10.1016/j.metabol.2018.09.005

Lin X, Li H. Obesity: Epidemiology, Pathophysiology, and Therapeutics. Front Endocrinol (Lausanne). 2021 Sep 6;12:706978. doi: https://doi.org/10.3389/fendo.2021.706978

Haverinen E, et al. Comparison of metabolic syndrome prevalence using four definitions – a case study from Finland. Eur J Public Health. 2021;31(Supple 1):9. doi: https://doi.org/10.1093/eurpub/ckab164.413

Mazur-Bialy AI. Asprosin – a fasting-induced, glucogenic, and orexigenic adipokine as a new promising player. Will it be a new factor in the treatment of obesity, diabetes, or infertility? a review of the literature. Nutrients. 2021 Feb 14;13(2):620. doi: https://doi.org/10.3390/nu13020620

Wang Y, Zhang L, Wu , Cao R, Peng X, Fu L. An Association Between FNDC5, PGC-1α Genetic Variants and Obesity in Chinese Children: A Case-Control Study. Diabetes Metab Syndr Obes. 2023 Jan 11;16:47-59. doi: https://doi.org/10.2147/DMSO.S391219

Tu WJ, Qiu HC, Cao JL, Liu Q, Zeng XW, Zhao JZ. Decreased Concentration of Irisin Is Associated with Poor Functional Outcome in Ischemic Stroke. Neurotherapeutics. 2018 Oct;15(4):1158-67. doi: https://doi.org/10.1007/s13311-018-0651-2

Gheit REAE, Younis RL, El-Saka MH, Emam MN, Soliman NA, El-Sayed RM. Irisin improves adiposity and exercise tolerance in a rat model of postmenopausal obesity through enhancing adipo-myocyte thermogenesis. J Physiol Biochem. 2022 Nov;78(4):897-913. doi: https://doi.org/10.1007/s13105-022-00915-3

Pinho-Jr JDS, Camacho FA, Cavararo CDS, Baião PF, Medeiros RF, Barroso SG, et al. Irisin and Cardiometabolic Disorders in Obesity: A Systematic Review. Int J Inflam. 2023 Oct 19;2023:5810157. doi: https://doi.org/0.1155/2023/5810157

Akbulut T, Cinar V, Aydin S, Yardim M. The Role of Different Exercises in Irisin, Heat Shock Protein 70 and Some Biochemical Parameters. J Med Biochem. 2022 Apr 8;41(2):149-55. doi: https://doi.org/10.5937/jomb0-31551

D'Amuri A, Raparelli V, Sanz JM, Capatti E, Di Vece F, Vaccari F, et al. Biological Response of Irisin Induced by Different Types of Exercise in Obese Subjects: A Non-Inferiority Controlled Randomized Study. Biology (Basel). 2022 Mar 2;11(3):392. doi: https://doi.org/10.3390/biology11030392

Minuti A, Raffaele I, Scuruchi M, Lui M, Muscarà C, Calabrò M. Role and Functions of Irisin: A Pers¬pective on Recent Developments and Neu¬rodege-nerative Diseases. Antioxidants (Basel). 2025 May 7;14(5):554. doi: https://doi.org/10.3390/antiox14050554

Colpitts BH, Rioux BV, Eadie AL, Brunt KR, Sénéchal M. Irisin response to acute moderate intensity exercise and high intensity interval training in youth of different obesity statuses: A randomized crossover trial. Physiol Rep. 2022 Feb;10(4):e15198. doi: https://doi.org/10.14814/phy2.15198

Tommasini E, Missaglia S, Vago P, Galvani C, Pecci C, Rampinini E, et al. The time course of irisin release after an acute exercise: relevant implications for health and future experimental designs. Eur J Transl Myol. 2024 Jul 1;34(2):12693. doi: https://doi.org/10.4081/ejtm.2024.12693

Dünnwald T, Melmer A, Gatterer H, Salzmann K, Ebenbichler C, Burtscher M, et al. Supervised Short-term High-intensity Training on Plasma Irisin Concentrations in Type 2 Diabetic Patients. Int J Sports Med. 2019 Mar;40(3):158-64.

doi: https://doi.org/10.1055/a-0828-8047

Enteshary M, Esfarjani F, Reisi J. Comparison of the effects of two different intensities of combined training on irisin, betatrophin, and insulin levels in women with type 2 diabetes. Asian J Sports Med. 2019;109:2. doi: https://doi.org/10.5812/asjsm.68943

Yang D, Li Y, Fan X, Liang H, Han R. The impact of exercise on serum irisin, osteocalcin, and adiponectin levels and on glycolipid metabolism in patients with type 2 diabetes. Int J Clin Exp Med [Internet]. 2020 [cited 2025 Aug 12];13(10):7816-24. Available from:

https://e-century.us/files/ijcem/13/10/ijcem0113138.pdf

Sari AR, et al. Impact of Time-Resricted Feeding and Aerobic Exercise Combination on Promotes Myokine Levels and Improve Body Composition in Obese Women. Retos. 2024;53:1-10. doi: https://doi.org/10.47197/retos.v53.102429

Amanat S, Sinaei E, Panji M, Mohammadpor Hodki R, Bagheri-Hosseinabadi Z, Asadimehr H, et al. A Randomized Controlled Trial on the Effects of 12 Weeks of Aerobic, Resistance, and Combined Exercises Training on the Serum Levels of Nesfatin-1, Irisin-1 and HOMA-IR. Front Physiol. 2020 Oct 16;11:562895. doi: https://doi.org/10.3389/fphys.2020.562895

Batumi A. The Changes of Irisin Levels in Athletes after Eight Weeks Intensity Internal Training. Journal of Humanities Insights. 2020;4(1):41-4. doi: https://doi.org/10.22034/jhi.2020.105799

Dündar A. Effect of acute handball training on irisin, leptin and some biochemical parameters for adoles-cence handball players. Univers J Educ Res. 2019;7(2):318-22. doi: https://doi.org/10.13189/ujer.2019.070202

Planella-Farrugia C, Comas F, Sabater-Masdeu M, Moreno M, Moreno-Navarrete JM, Rovira O, et al. Circulating irisin and myostatin as markers of muscle strength and physical condition in elderly subjects. Front Physiol. 2019 Jul 10;10:871. doi: https://doi.org/10.3389/fphys.2019.00871

Piccirillo R. Exercise-induced myokines with therapeutic potential for muscle wasting. Front Physiol. 2019 Mar 29;10:287. doi: https://doi.org/10.3389/fphys.2019.00287

Wang F, Wang X, Liu Y, Zhang Z. Effects of Exercise-Induced ROS on the Pathophysiological Functions of Skeletal Muscle. Oxid Med Cell Longev. 2021 Oct 1;2021:3846122. doi: https://doi.org/10.1155/2021/3846122

Hargreaves M, Spriet LL. Skeletal muscle energy metabolism during exercise. Nat Metab. 2020 Sep;2(9):817-28. doi: https://doi.org/10.1038/s42255-020-0251-4

Fritzen AM, Lundsgaard AM, Kiens B. Tuning fatty acid oxidation in skeletal muscle with dietary fat and exercise. Nat Rev Endocrinol. 2020 Dec;16(12):683-96. doi: https://doi.org/10.1038/s41574-020-0405-1

Muscella A, Stefàno E, Lunetti P, Capobianco L, Marsigliante S. The regulation of fat metabolism during aerobic exercise. Biomolecules. 2020 Dec 21;10(12):1699. doi: https://doi.org/10.3390/biom10121699

Mozaffaritabar S, Koltai E, Zhou L, Bori Z, Kolonics A, Kujach S, et al. PGC-1α activation boosts exercise-dependent cellular response in the skeletal muscle. J Physiol Biochem. 2024 May;80(2):329-35. doi: https://doi.org/10.1007/s13105-024-01006-1

Thirupathi A, da Silva Pieri BL, Queiroz JAMP, Rodrigues MS, de Bem Silveira G, de Souza DR, et al. Strength training and aerobic exercise alter mitochondrial parameters in brown adipose tissue and equally reduce body adiposity in aged rats. J Physiol Biochem. 2019 Feb;75(1):101-8. doi: https://doi.org/10.1007/s13105-019-00663-x

Lin J, Liu X, Zhou Y, Zhu B, Wang Y, Cui W, et al. Molecular Basis of Irisin Regulating the Effects of Exercise on Insulin Resistance. Appl Sci. 2022;12(12):5837. doi: https://doi.org/10.3390/app12125837

Cheng CF, Ku HC, Lin H. Pgc-1α as a pivotal factor in lipid and metabolic regulation. Int J Mol Sci. 2018 Nov 2;19(11):3447. doi: https://doi.org/10.3390/ijms19113447

Rabiee F, Lachinani L, Ghaedi S, Nasr-Esfahani MH, Megraw TL, Ghaedi K. New insights into the cellular activities of Fndc5/Irisin and its signaling pathways. Cell Biosci. 2020 Mar 30;10:51. doi: https://doi.org/10.1186/s13578-020-00413-3

Arhire LI, Mihalache L, Covasa M. Irisin: A Hope in Understanding and Managing Obesity and Metabolic Syndrome. Front Endocrinol (Lausanne). 2019 Aug 2;10:524. doi: https://doi.org/10.3389/fendo.2019.00524

Flori L, Testai L, Calderone V. The ‘irisin sys¬tem’: From biological roles to pharmacological and nutraceutical perspectives. Life Sci. 2021 Feb 15;267:118954. doi: https://doi.org/10.1016/j.lfs.2020.118954

Catalano-Iniesta L, Sánchez Robledo V, Iglesias-Osma MC, Galán Albiñana A, Carrero S, Blanco EJ, et al. Evidences for expression and location of ANGPTL8 in human adipose tissue. J Clin Med. 2020 Feb 13;9(2):512. doi: https://doi.org/10.3390/jcm9020512

Dianatinasab A, Koroni R, Bahramian M, Bagheri-Hosseinabadi Z, Vaismoradi M, Fararouei M, et al. The effects of aerobic, resistance, and combined exercises on the plasma irisin levels, HOMA-IR, and lipid profiles in women with metabolic syndrome: A randomized controlled trial. J Exerc Sci Fit. 2020 Sep;18(3):168-76. doi: https://doi.org/10.1016/j.jesf.2020.06.004

Jagsz S, Sikora M. The Effectiveness of High-Intensity Interval Training vs. Cardio Training for Weight Loss in Patients with Obesity: A Systematic Review. J Clin Med. 2025 Feb 15;14(4):1282. doi: https://doi.org/10.3390/jcm14041282