CD117 expression on blast cells in acute myeloid leukemia.

N. V. Goryainova; A. I. Gordienko; V. A. Kubarova

doi:10.26641/2307-0404.2015.3.53688

Автор(и)

N. V. Goryainova
A. I. Gordienko
V. A. Kubarova

DOI:

https://doi.org/10.26641/2307-0404.2015.3.53688

Ключові слова:

acute myeloid leukemia, blast cells, immunophenotyping, target therapy, CD117

Анотація

The aim of the present work was to analyze the frequency of CD117 (c-KIT) antigen expression on the blast cells in acute myeloid leukemia (AML), evaluation of the presence of the relationship between the expression of the c-KIT and leukemia according to the FAB classification and definition of co-expression of the antigen CD117, antigens CD33 and CD34. The data of 47 patients with AML were diagnosed. M0 AML variant was established in 3 (6%) patients, M1 – in 2 (4%), M2 – in 9 (20%), M4 – in 22 (47%) and M5 – in 11 (23%). For immunophenotypic studies monoclonal antibodies (mAb) that detect antigens of anti-CD34, anti-CD33 and anti-CD117 (Becton Dickinson, USA) were used. The presence of the antigen CD117 was detected in 39 people, accounting for 83% of all surveyed. Antigen c-KIT was present in 48.1±17.0% cells on average: in all 3 cases – AML M0, in2 cases of AML M1, in 6 cases – AML M2, 20 of 22 cases – AML M4 and in 8 of 11 AML M5 cases. Average levels of CD117 in investigated leukemia cases statistically differed significantly (p=0.0067). Among 39 CD117- positive patients in 25 (53%) co-expression of CD117+/CD34+ was revealed. Expression of CD117+/CD34- was observed in 14 cases (30%), CD117-/CD34+ – in 4 cases (8,5%), CD117-/CD34- – in 4 cases (8.5%). CD34 had of 64% of cells of myeloid origin. A high positive correlation between expression of CD117 and CD34 (r=+0,5169) was determined, being statistically significant (p<0.0067). CD33 antigen was present on 81% of the studied cells. A weak negative correlation between expression of CD117 and CD33 (r=-0,2247), was revealed, being statistically significant (p>0,0067).

Біографії авторів

N. V. Goryainova

SI "Institute of Hematology and Transfusiology of NAMS of Ukraine"
M. Berlynskogo st., 12, Kyiv, 02121,Ukraine

A. I. Gordienko

SI "Institute of Hematology and Transfusiology of NAMS of Ukraine"
M. Berlynskogo st., 12, Kyiv, 02121,Ukraine

V. A. Kubarova

SI "Institute of Hematology and Transfusiology of NAMS of Ukraine"
M. Berlynskogo st., 12, Kyiv, 02121,Ukraine

Посилання

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