Recurrent pregnancy loss association with allelic variants of IL8 and IL10 genes

Anastasiia Kucherenko; Roman Gulkovskyi; Kseniya Hazhylenko; Iryna Vorobiova; Tamara Nakvasiuk; Ludmila Livshits

doi:10.15587/2313-8416.2014.27254

Authors

Anastasiia Kucherenko Taras Shevchenko National University of Kyiv Glushkova ave., Kyiv, Ukraine, 03022, Ukraine
Roman Gulkovskyi Institute of Molecular Biology and Genetics of NAS of Ukraine 150 Zabolotnogo str., Kyiv, Ukraine, 03680, Ukraine
Kseniya Hazhylenko Obstetric – gynecological clinic “ISIDA” 65, Lepse blvd, Kyiv, Ukraine, 03000, Ukraine
Iryna Vorobiova SI “Institute of Pediatrics, Obstetrics and Gynecology of NAMS of Ukraine” 8 Maiborody str., Kyiv, Ukraine, 04050, Ukraine
Tamara Nakvasiuk SI “Institute of Pediatrics, Obstetrics and Gynecology of NAMS of Ukraine” 8 Maiborody str., Kyiv, Ukraine, 04050, Ukraine
Ludmila Livshits Institute of Molecular Biology and Genetics of NAS of Ukraine 150 Zabolotnogo str., Kyiv, Ukraine, 03680, Ukraine

DOI:

https://doi.org/10.15587/2313-8416.2014.27254

Keywords:

IL8 gene/ IL10 gene/ interleukin/ recurrent pregnancy loss

Abstract

The study evaluated IL8 -781C/T and IL10 -592C/A, -1082A/G polymorphisms association with recurrent pregnancy loss (RPL) (n=110 case, n=106 control).

IL10 -592АА genotype and -1082A allele carriers frequency was higher in RPL group (6.6 %, 87.3 %) comparing to control (0.9 %, 76.4 %). The -1082A allele carriers have twice increased RPL risk (OR=2.12; CI95 %: 1,03–4,34).

Author Biographies

Anastasiia Kucherenko, Taras Shevchenko National University of Kyiv Glushkova ave., Kyiv, Ukraine, 03022

PhD student, junior research fellow

Roman Gulkovskyi, Institute of Molecular Biology and Genetics of NAS of Ukraine 150 Zabolotnogo str., Kyiv, Ukraine, 03680

PhD student, junior research fellow

Department of general and molecular genetics

Kseniya Hazhylenko, Obstetric – gynecological clinic “ISIDA” 65, Lepse blvd, Kyiv, Ukraine, 03000

Obstetrician/gynecologist of Superior Expert Category, geneticist

Iryna Vorobiova, SI “Institute of Pediatrics, Obstetrics and Gynecology of NAMS of Ukraine” 8 Maiborody str., Kyiv, Ukraine, 04050

M.D., obstetrician/gynecologist of Superior Expert Category, Head of the Department

Department of scientific problems of pregnancy loss

Tamara Nakvasiuk, SI “Institute of Pediatrics, Obstetrics and Gynecology of NAMS of Ukraine” 8 Maiborody str., Kyiv, Ukraine, 04050

PhD student

Department of scientific problems of pregnancy loss

Ludmila Livshits, Institute of Molecular Biology and Genetics of NAS of Ukraine 150 Zabolotnogo str., Kyiv, Ukraine, 03680

PhD, Professor, Head of the Department

Human genomics department

References

1. Branch, D. W., Gibson, M., Silver, R. M. (2010). Clinical practice. Recurrent miscarriage. New England Journal of Medicine, 363 (18), 1740–1747. doi: 10.1056/NEJMcp1005330

2. Lim, K. J. H., Odukoya, O. A., Li, T. C., Cooke, I. D. (1996). Cytokines and immuno-endocrine factors in recurrent miscarriage. Human Reproduction Update, 2 (6), 469–481. doi: 10.1093/humupd/2.6.469

3. Saini, V., Arora, S., Yadav, A., Bhattacharjee, J. (2011). Cytokines in recurrent pregnancy loss. Clinica Chimica Acta, 412 (9-10), 702–708. doi: 10.1016/j.cca.2011.01.002

4. Dealtry, G. B., O’Farrell, M. K., Fernandez, N. (2000). The Th2 cytokine environment of the placenta. International Archives of Allergy and Immunology, 123 (2), 107–119. doi: 10.1159/000024441

5. Daly, A. K., Day, C. P., Donaldson, P. T. (2002). Polymorphisms in immunoregulatory genes: towards individualized immunosuppressive therapy? American Journal of Pharmacogenomics, 2 (1), 13–23. doi: 10.2165/00129785-200202010-00002

6. Emonts, M., Hazes, M. J., Houwing-Duistermaat, J. J., van der Gaast-de Jongh, C. E., de Vogel, L., Han, H. K., Wouters, J. M., Laman, J. D., Dolhain, R. J. (2011). Polymorphisms in genes controlling inflammation and tissue repair in rheumatoid arthritis: a case control study. BMC Medical Genetics, 12 (1), 36. doi: 10.1186/1471-2350-12-36

7. Heinzmann, A., Ahlert, I., Kurz, T., Berner, R., Deichmann, K. A. (2004). Association study suggests opposite effects of polymorphisms within IL8 on bronchial asthma and respiratory syncytial virus bronchiolitis. Journal of Allergy and Clinical Immunology, 114 (3), 671–676. doi: 10.1016/j.jaci.2004.06.038

8. Weimar, C. H., Macklon, N. S., Post Uiterweer, E. D., Brosens, J. J., Gellersen, B. (2013). The motile and invasive capacity of human endometrial stromal cells: implications for normal and impaired reproductive function. Human Reproduction Update, 19 (5), 542–557. doi: 10.1093/humupd/dmt025

9. The GeneCards human gene. Available at: http://www.genecards.org/cgi-bin/carddisp.pl?gene=il8.

10. The GeneCards human gene. Available at: http://www.genecards.org/cgi-bin/carddisp.pl?gene=il10.

11. Hacking, D., Knight, J. C., Rockett, K., Brown, H., Frampton, J., Kwiatkowski, D. P., Hull, J., Udalova, I. A. (2004). Increased in vivo transcription of an IL-8 haplotype associated with respiratory syncytial virus disease-susceptibility. Genes Immun, 5 (4), 274-282. doi: 10.1038/sj.gene.6364067

12. Temple, S. E., Lim, E., Cheong, K. Y., Almeida, C. A., Price, P., Ardlie, K. G., Waterer, G. W. (2003). Alleles carried at positions -819 and -592 of the IL10 promoter affect transcription following stimulation of peripheral blood cells with Streptococcus pneumoniae. Immunogenetics, 55 (9), 629–632. doi: 10.1007/s00251-003-0621-6

13. Choi, Y. K., Kwak-Kim, J. (2008). Cytokine gene polymorphisms in recurrent spontaneous abortions: a comprehensive review. American Journal of Reproductive Immunology, 60 (2), 91–110. doi: 10.1111/j.1600-0897.2008.00602.x

14. Costa, G. C., da Costa Rocha, M. O., Moreira, P. R., Menezes, C. A., Silva, M. R., Gollob, K. J., Dutra, W. O. (2009). Functional IL-10 gene polymorphism is associated with Chagas disease cardiomyopathy. Journal of Infectious Diseases, 199 (3), 451–454. doi: 10.1086/596061

15. Rousset, F. (2008). Genepop'007: a complete reimplementation of the Genepop software for Windows and Linux. Molecular Ecology Resources, 8 (1), 103–106. doi: 10.1111/j.1471-8286.2007.01931.x

16. Sullivan, K. M., Dean, A., Soe, M. M. (2009). OpenEpi: a web-based epidemiologic and statistical calculator for public health. Public Health Reports, 124 (3), 471–474.

17. Saito, S., Nakashima, A., Shima, T., Ito, M. (2010). Th1/Th2/Th17 and regulatory T-cell paradigm in pregnancy. American Journal of Reproductive Immunology, 63 (6), 601-610. doi: 10.1111/j.1600-0897.2010.00852.x

18. Krieg, S. A., Fan, X., Hong, Y., Sang, Q. X., Giaccia, A., Westphal, L. M., Lathi, R. B., Krieg, A. J., Nayak, N. R. (2012). Global alteration in gene expression profiles of deciduas from women with idiopathic recurrent pregnancy loss. Molecular Human Reproduction, 18 (9), 442–450. doi: 10.1093/molehr/gas017

19. Morelli, S. S., Keegan, D. A., Krey, L. C., Katz, J., Liu, M., Noyes, N. (2008). Early serum interleukin-8 evaluation may prove useful in localizing abnormally implanted human gestations after in vitro fertilization. Fertility and Sterility, 90 (6), 2068–2072. doi: 10.1016/j.fertnstert.2007.10.063

20. Piccinni, M. P., Beloni, L., Livi, C., Maggi, E., Scarselli, G., Romagnani, S. (1998). Defective production of both leukemia inhibitory factor and type 2 T-helper cytokines by decidual T cells in unexplained recurrent abortions. Nature Medicine, 4 (9), 1020-1024. doi: 10.1038/2006

21. Robertson, S. A., Care, A. S., Skinner, R. J. (2007). Interleukin 10 regulates inflammatory cytokine synthesis to protect against lipopolysaccharide-induced abortion and fetal growth restriction in mice. Biology of Reproduction, 76 (5), 738–748. doi: 10.1095/biolreprod.106.056143

22. Chaouat, G., Assal Meliani, A., Martal, J., Raghupathy, R., Elliott, J. F., Mosmann, T., Wegmann, T. G. (1995). IL-10 prevents naturally occurring fetal loss in the CBA x DBA/2 mating combination, and local defect in IL-10 production in this abortion-prone combination is corrected by in vivo injection of IFN-tau. The Journal of Immunology, 154 (9), 4261–4268.