New enzymatic method for l-arginine determination based on use of human arginase i and urease
DOI:
https://doi.org/10.15587/2313-8416.2015.45126Keywords:
enzymatic assay, arginase I, urease, o-Phthalaldehyde, L-arginine, urea, ammonium, spectrophotometryAbstract
It is developed a sensitive and selective method for quantitative analysis of L-arginine with spectrophotometric method of detection of the reaction product (ammonium) using o-Phthalaldehyde. The method is based on the use of highly purified arginase I and commercial urease. The new method is highly sensitive for L-arginine determination, a wide range of linearity and insensitivity to interfering influence of wide range of amino acids
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Copyright (c) 2015 Галина Зуфарівна Гайда, Наталія Євгенівна Стасюк, Суренівна Єпрємян Хасмік, Михайло Васильович Гончар, Софія Ростиславівна Басс
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