Новий ензиматичний метод визначення L-аргініну за використання аргінази і людини та уреази
DOI :
https://doi.org/10.15587/2313-8416.2015.45126Mots-clés :
ензиматичний аналіз, аргіназа I, уреаза, о-фталевий альдегід, L-аргінін, сечовина, амоній, спектрофотометріяRésumé
Розроблено чутливий та селективний метод кількісного аналізу L-Аргініну із спектрофотометричним способом детектування продукту реакції (амонію) за допомогою о-фталевого альдегіду. Метод базується на використанні високоочищеної аргінази І та комерційної уреази. Новому методу властива висока порогова чутливість визначення L-Аргініну, широкий діапазон лінійності, а також нечутливість до інтерферуючого впливу широкого спектру амінокислот
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(c) Tous droits réservés Галина Зуфарівна Гайда, Наталія Євгенівна Стасюк, Суренівна Єпрємян Хасмік, Михайло Васильович Гончар, Софія Ростиславівна Басс 2015
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