Diabetes-induced impairments in renal cortex of rats: effect of nicotinamide treatment


  • Леся Василівна Яніцька National Medical University of Ukraine 34 Victory ave., Kiev, Ukraine, 01061, Ukraine




renal cortex, diabetes, nephropathy, nicotinamide, NAD, ratio, rats, experiment, model, NAD(P)/NAD(P)H pairs


There was offered the methods of study of nicotinamide administration modeling effect (invivo) that can be realized by means of NAD and is capable to get combined with renal cortex membranes in a specific way.

The aim of research was to explore is the content of NAD and NADP and free NAD(P)/NAD(P)H pairs ratio in renal cortex changes at diabetes mellitus and nicotinamide effect.

Methods. 50 rats-males of Wistar line weighing 180–210 g. with experimental diabetes mellitus type 1 caused by single intra-abdominal administration of streptozotocin, dose – 60 mg. for 1 kg. of body weight. Animals were separated into 3 groups – the control one (C), the group of rats with diabetes mellitus type 1 (D) and rats with diabetes that underwent administration of Nam (nicotinamide) («Sigma», США), dose – 100 mg\kg of body weight during 14 days. The glucose concentration was defined using glucometer «Accu-chek» (Roshediagnostics, Swizerland).

Results. According to the data received NAD level in renal cortex was reduced to 0,179±0,012 mmol/g at diabetes against 0,259±0,023 mmol/g of tissue, Р<0,05 in the control. The NAD(P)/NAD(P)H free pairs ratio reduced to 202.0±16,1 and 0,008±0,001 in renal cortex at diabetes against 297.0±21,2 and 0.013±0.002 in the control for NAD and NADP respectively. Nicotinamide administration resulted in partial renewal of NAD level in renal cortex and NAD(P)/ NAD(P)H free pairs ratio. The modeling effect invivo of administered nicotinamide can be realized by means of NAD that is capable to get combined in renal cortex membranes in a specific way.

Conclusions. So nicotonamide takes part in regulation of kidney processes that indicates its usefulness for diabetes nephropathy treatment

Author Biography

Леся Василівна Яніцька, National Medical University of Ukraine 34 Victory ave., Kiev, Ukraine, 01061

Candidate of Biological Sciences.

Department of Bioorganic and Biological Chemistry


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Medical Science. Part 1