Assay of L-Arginine in rat blood by enzymatic method
DOI:
https://doi.org/10.15587/2313-8416.2015.54486Keywords:
Аrginine, urea, rat blood, enzymatic-chemical assay, arginase, 2, 3-butandion monooximAbstract
The study of enzymatic-chemical method of L-Arginine (Arg) assay on the real samples of rat blood was done. The developed method is based on the usage of recombinant arginase I and 2,3-butandion monooxim. Urea, the final product of the reaction, is detected by fluorometry. The new method was shown to be suitable for Arg assay in biological liquids of mammalians
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Copyright (c) 2015 Галина Зуфарівна Гайда, Наталія Євгенівна Стасюк, Наталія Олександрівна Cибірна, Михайло Васильович Гончар
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