Using of purified platelets for investigation of “cell-efector” model in hemostasis system

Authors

  • Тетяна Іванівна Галенова Taras Shevchenko National University of Kyiv Volodymirska str., 64/13, Kyiv, Ukraine, 01601, Ukraine
  • Наталія Миколаївна Pocлова Taras Shevchenko National University of Kyiv Volodymirska str., 64/13, Kyiv, Ukraine, 01601, Ukraine
  • Олексій Миколайович Савчук Taras Shevchenko National University of Kyiv Volodymirska str., 64/13, Kyiv, Ukraine, 01601, Ukraine

DOI:

https://doi.org/10.15587/2519-8025.2016.83427

Keywords:

platelet purification, gel filtration on Sepharose 4B, aggregation, electrophoresis

Abstract

Gel filtration on a column of Sepharose 4B was tested as method for separating platelet fraction. The results of cytological and electrophoretic analysis showed absence of other blood cells and plasma proteins in the content of the resulting cell fraction. Obtained platelets were proved to keep morphological stability and retain the ability to aggregate in response to ADP – one of the main physiological inductors

Author Biographies

Тетяна Іванівна Галенова, Taras Shevchenko National University of Kyiv Volodymirska str., 64/13, Kyiv, Ukraine, 01601

PhD, senior researcher

Department of Biochemistry

Наталія Миколаївна Pocлова, Taras Shevchenko National University of Kyiv Volodymirska str., 64/13, Kyiv, Ukraine, 01601

Senior engineer

Олексій Миколайович Савчук, Taras Shevchenko National University of Kyiv Volodymirska str., 64/13, Kyiv, Ukraine, 01601

Doctor of Biological Science, professor

Department of Biochemistry

References

  1. Ghoshal, K., Bhattacharyya, M. (2014). Overview of Platelet Physiology: Its Hemostatic and Nonhemostatic Role in Disease Pathogenesis. The Scientific World Journal, 2014, 1–16. doi: 10.1155/2014/781857
  2. Ribatti, D., Crivellato, E. (2007). Giulio Bizzozero and the discovery of platelets. Leukemia Research, 31 (10), 1339–1341. doi: 10.1016/j.leukres.2007.02.008
  3. Patel, S. R. (2005). The biogenesis of platelets from megakaryocyte proplatelets. Journal of Clinical Investigation, 115 (12), 3348–3354. doi: 10.1172/jci26891
  4. Daly, M. E. (2010). Determinants of platelet count in humans. Haematologica, 96 (1), 10–13. doi: 10.3324/haematol.2010.035287
  5. Packham, M. A. (1994). Role of platelets in thrombosis and hemostasis. Canadian Journal of Physiology and Pharmacology, 72 (3), 278–284. doi: 10.1139/y94-043
  6. Guyton, A. C., Hall, J. E. (2005). Textbook of Medical Physiology. Chap. 36. Hemostasis and Blood Coagulation. Elsevier, 457–467.
  7. Kauskot, A., Hoylaerts, M. F. (2012). Platelet Receptors. Handbook of Experimental Pharmacology, 23–57. doi: 10.1007/978-3-642-29423-5_2
  8. Munnix, I. C. A., Cosemans, J. M. E. M., Auger, J. M., Heemskerk, J. W. M. (2009). Platelet response heterogeneity in thrombus formation. Thrombosis and Haemostasis. doi: 10.1160/th09-05-0289
  9. Steinhubl, S. R., Moliterno, D. J. (2005). The Role of the Platelet in the Pathogenesis of Atherothrombosis. American Journal of Cardiovascular Drugs, 5 (6), 399–408. doi: 10.2165/00129784-200505060-00007
  10. Heemskerk, J. W., Bevers, E. M., Lindhout, T. (2002). Platelet activation and blood coagulation. Thromb. Haemost., 88 (2), 186–193.
  11. Flaumenhaft, R. (2003). Molecular Basis of Platelet Granule Secretion. Arteriosclerosis, Thrombosis, and Vascular Biology, 23 (7), 1152–1160. doi: 10.1161/01.atv.0000075965.88456.48
  12. Rumbaut, R. E., Thiagarajan, P. (2010). Platelet-vessel wall interactions in hemostasis and thrombosis. Chap. 5. Platelet recruitment and blood coagulation. Morgan & Claypool, 29–35.
  13. Esmon, C. T. (2009). Basic mechanisms and pathogenesis of venous thrombosis. Blood Reviews, 23 (5), 225–229. doi: 10.1016/j.blre.2009.07.002
  14. Cerletti, C., Tamburrelli, C., Izzi, B., Gianfagna, F., De Gaetano, G. (2012). Platelet-leukocyte interactions in thrombosis. Thrombosis Research, 129 (3), 263–266. doi: 10.1016/j.thromres.2011.10.010
  15. Connolly, A. J., Ishihara, H., Kahn, M. L., Farese, R. V., Coughlin, S. R. (1996). Role of the thrombin receptor in development and evidence for a second receptor. Nature, 381 (6582), 516–519. doi: 10.1038/381516a0
  16. Kisucka, J., Butterfield, C. E., Duda, D. G., Eichenberger, S. C., Saffaripour, S., Ware, J. et. al. (2006). Platelets and platelet adhesion support angiogenesis while preventing excessive hemorrhage. Proceedings of the National Academy of Sciences, 103 (4), 855–860. doi: 10.1073/pnas.0510412103
  17. Paidas, M. J., Haut, M. J., Lockwood, C. J. (1994). Platelet disorders in pregnancy: implications for mother and fetus. Mt. Sinai J. Med., 61 (5), 389–403.
  18. Belloc, C., Lu, H., Soria, C., Fridman, R., Legrand, Y., Menashi, S. (1995). The effect of platelets on invasiveness and protease production of human mammary tumor cells. International Journal of Cancer, 60 (3), 413–417. doi: 10.1002/ijc.2910600324
  19. Ruf, W., Mueller, B. (2006). Thrombin Generation and the Pathogenesis of Cancer. Seminars in Thrombosis and Hemostasis, 32 (S1), 061–068. doi: 10.1055/s-2006-939555
  20. Jain, S., Harris, J., Ware, J. (2010). Platelets: Linking Hemostasis and Cancer. Arteriosclerosis, Thrombosis, and Vascular Biology, 30 (12), 2362–2367. doi: 10.1161/atvbaha.110.207514
  21. Cleveland, D. W. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. Journal of Biological Chemistry, 252 (3), 1102-1106.
  22. Harrison, P., Mackie, I., Mumford, A., Briggs, C., Liesner, R., Winter, M., Machin, S. (2011). Guidelines for the laboratory investigation of heritable disorders of platelet function. British Journal of Haematology, 155 (1), 30–44. doi: 10.1111/j.1365-2141.2011.08793.x
  23. Timmons, S., Hawiger, J. (1989). Isolation of human platelets by albumin gradient and gel filtration. Platelets: Receptors, Adhesion, Secretion Part A, 11–21. doi: 10.1016/0076-6879(89)69046-5
  24. Groscurth, P., Cheng, S., Vollenweider, I., von Felten, A. (2009). Effects of Washing and Gel Filtration on the Ultrastructure of Human Platelets. Acta Haematologica, 77 (3), 150–155. doi: 10.1159/000205980
  25. Jesty, J., Bluestein, D. (1999). Acetylated Prothrombin as a Substrate in the Measurement of the Procoagulant Activity of Platelets: Elimination of the Feedback Activation of Platelets by Thrombin. Analytical Biochemistry, 272 (1), 64–70. doi: 10.1006/abio.1999.4148
  26. Moran, N., Kiernan, A., Dunne, E., Edwards, R. J., Shields, D. C., Kenny, D. (2006). Monitoring modulators of platelet aggregation in a microtiter plate assay. Analytical Biochemistry, 357 (1), 77–84. doi: 10.1016/j.ab.2006.06.037
  27. Katrii, T. B., Vovk, T. B., Kravchenko, N. K., Savchuk, O. M., Ostapchenko, L. I. (2015). Influence of IgG Separated from Blood Plasma of Patients with Ischemic Stroke on the Process Platelet's Proteins Secretion. Intern. J. Chem. Biomol. Science, 1 (4), 278–283.

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Published

2016-11-18

How to Cite

Галенова, Т. І., Pocлова Н. М., & Савчук, О. М. (2016). Using of purified platelets for investigation of “cell-efector” model in hemostasis system. ScienceRise: Biological Science, (3 (3), 4–9. https://doi.org/10.15587/2519-8025.2016.83427

Issue

No. 3 (3) (2016)

Section

Biological Sciences

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Copyright (c) 2016 Тетяна Іванівна Галенова, Наталія Миколаївна Pocлова, Олексій Миколайович Савчук

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