Morphometric parameters of hepatocytes in experimental complete extrahepatic bile duct obstruction

Authors

DOI:

https://doi.org/10.15587/2519-4798.2020.193845

Keywords:

experimental biliary tract obstruction, cholestasis, hepatocyte, liver morphology, morphometry

Abstract

Liver changes observed in complete extrahepatic bile duct obstruction (CEBDO) with consideration to its morphometric parameters, may reflect the upcoming decompensation of liver function and may serve as objective criteria for the disease prognosis.

The aim. To study the morphological changes of hepatocytes in experimental CEBDO using macro- and micromorphometry.

Materials and methods. In 41 rats, the CEBDO was done by ligation and transection of the common bile duct. The time points were on postoperative Day 1, 3, 7, 14, 21, 28 and 35. Control group included 10 non-operated rats. The total blood bilirubin (TB), liver volume (LV), the area of hepatocytes (AH), the hepatocyte nuclear-cytoplasmic ratio (HNCR), the hepatocyte bulk density (HBD) were investigated. The LV and HBD parameters were used to calculate the total volume of hepatocytes (TVH).

Results. The highest mortality was recorded on Day 22-35 of the experiment (7 out of 11 animals). Bilirubin level was significantly higher than in the control group with its maximum on Day 1 (295±100 vs. 8±6, μmol/l, p<0.001). The highest LV value was observed on Day 14 (14.1±1.1 vs. 8.4±1.4cm³, p<0.01). The processes prevailing in the livers were cell proliferation, fibrosis with gradual displacement of hepatocytes by proliferating bile ducts, and complete loss of normal liver histostructure. The proportion of hepatocytes in the liver (HBD) was progressively decreasing from 0.94 (Control) to 0.44 (Day 35). In spite of that, the TVH level was initially increased (max 9.7±0.36cm³ on Day 3 vs. 8.3±0.26cm³ on Day 1, p<0.05), but after Day 14 it decreased, with no significant differences from the Control group on Day 21(8.2±1.2 vs. 7.9±1.6cm³, p>0.05), with its lowest level (5.0±0.9cm³) on Day 35 (p<0.05 compared with max value on Day 3). The HNCR index, which reflects proliferative activity of hepatocytes, had its maximum on Day 14 (0.53±0.01 vs. 0.21±0.05 in Control group, p<0.001).

Conclusions. In experimental CEBDO, reduction of the HBD index less than 60 % and reduction of TVH less than the value of a normal liver are accompanied by highest mortality, i.e., are a sign of hepatic decompensation. That was preceded by the maximum proliferative activity of hepatocytes, the criterion of which is the HNCR index

Author Biographies

Ivan Mamontov, Kharkiv Medical Academy of Postgraduate Education Amosova str., 58, Kharkiv, Ukraine, 61176

PhD, Associate Professor

Department of Surgery and Proctology

Igor Ivakhno, Kharkiv City Clinical Hospital No. 17 Moskovsky ave., 195, Kharkiv, Ukraine, 61037 Kharkiv Medical Academy of Postgraduate Education Amosova str., 58, Kharkiv, Ukraine, 61176

PhD, Teaching Assistant, Doctor-Pathologist

Department of pathology

Tamara Tamm, Kharkiv Medical Academy of Postgraduate Education Amosova str., 58, Kharkiv, Ukraine, 61176

MD, Professor, Head of Department

Department of Surgery and Proctology

V’yacheslav Panasenko, Kharkiv National Medical University Nauky ave., 4, Kharkiv, Ukraine, 61022

Assistant

Department of Histology, Cytology and Embryology

Volodymyr Padalko, V. N. Karazin Kharkiv National University Svobody sq., 4, Kharkiv, Ukraina, 61022

PhD, Senior Researcher, Associate Professor

Department of General and Clinical Pathology

Ismat Zulfugarov, Kharkiv Medical Academy of Postgraduate Education Amosova str., 58, Kharkiv, Ukraine, 61176

Postgraduate student

Department of surgery and proctology

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Published

2020-02-06

How to Cite

Mamontov, I., Ivakhno, I., Tamm, T., Panasenko, V., Padalko, V., & Zulfugarov, I. (2020). Morphometric parameters of hepatocytes in experimental complete extrahepatic bile duct obstruction. ScienceRise: Medical Science, (1 (34), 51–56. https://doi.org/10.15587/2519-4798.2020.193845

Issue

No. 1 (34) (2020)

Section

Medical Science

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Copyright (c) 2020 Ivan Mamontov, Igor Ivakhno, Tamara Tamm, V’yacheslav Panasenko, Volodymyr Padalko, Ismat Zulfugarov

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