Study of the effect of the xenobiotic hormone on the reproductive function of rats
DOI:
https://doi.org/10.15587/2519-4798.2020.212118Keywords:
Nonylphenol, reproductive system, gonads, spermatogenesisAbstract
The aim is to study the effect of the xenobiotic of Nonylphenol on functional and morphological indices of the reproductive system of males of rats.
Materials and methods. Experimental modelling of gonadotoxic effects of the xenobiotic was performed by inhalation of Nonylphenol at a concentration of 500 mg/m3 for 30 days, 5 days a week, 5 hours a day in conditions of the seed chamber. Male rats ejaculate were obtained by the method of electrostimulation seed tubercle in the mucous membrane of the rectum. The number of sperm in the ejaculate and their mobility were determined in the Hemocytometer 1, 7, 14 and 30 days after the start of the experiment. Macroscopic study of the testes included the assessment of external signs of damage, the determination of the mass and length of the testes.
Results. It is established that the introduction of Nonylphenol to rats males leads to a gradual dynamical reduction of the total number and the number of mobile forms of sperm cells compared to intact animals. At the end of the experiment the number of mobile forms of sperm was 2.1 times below the original data. Long-term effect of Nonylphenol has also led to a significant decrease of body weight growth, which confirms its high toxicity. In the study found that intoxication in Mature rats Nonylphenol were significant changes in the reproductive system, which is primarily characterized by decreased weight of the testes and the appendages of the testis, and changes in linear dimensions of the bodies downward. These violations indicate a feminizing effect of this hormone-like xenobiotic and its role in the development of male infertility.
Conclusions. Quantitative and qualitative parameters of the ejaculate of sexually mature male rats can serve as a valid criterion for adaptive and maladaptive processes occurring in the organism under the influence of the xenobiotic. In conditions of prolonged exposure to Nonylphenol (within 30 days) the changes in mass of the testes and reduce their linear dimensionsReferences
- Nikitin, A. I., Sergeev, O. V., Suvorov, A. N. (2016). Vliianie vrednykh faktorov sredy na reproduktivnuiu, endokrinnuiu sistemy i epigenom. Moscow: «Akvarel», 348.
- Veeramachaneni, D. N. R., Klinefelter, G. R. (2014). Phthalate-induced pathology in the foetal testis involves more than decreased testosterone production. Reproduction, 147 (4), 435–442. doi: http://doi.org/10.1530/rep-13-0441
- Lu, X., Liu, Y., Kong, X., Lobie, P. E., Chen, C., Zhu, T. (2013). Nanotoxicity: A Growing Need for Study in the Endocrine System. Small, 9 (9-10), 1654–1671. doi: http://doi.org/10.1002/smll.201201517
- Reznikov, A. G. (2014). Reproduktivnye misheni endokrinnykh dizraptorov. Reproduktivnaia endokrinologiia, 3 (17), 14–21.
- Stus, V. P., Polion, N. Iu., Salkova, N. V., Gubar, I. A. (2014). Biomonitoring tiazhelykh metallov v krovi i eiakuliate muzhchin s idiopaticheskim besplodiem. Urologіia, 18 (1), 31–35.
- Khliakina, O. V., Zakhriapina, L. V., Gulin, A. V., Agadzhanian, N. A. (2010). Osobennosti razvitiia narushenii fertilnosti u muzhchin reproduktivnogo vozrasta v zavisimosti ot urovnia antropotekhnogennoi nagruzki regiona prozhivaniia. Vestnik TGU, 15 (5), 1531–1534.
- Vitrischak, S. V., Bondarenko, V. V., Izorkina, I. I., Gavrik, S. Iu., Bondarenko, M. V., Sanina, E. V. (2013). Vozdeistvie toluola na organizm cheloveka i mery profilaktiki. Ukrainskii zhurnal klinichnoi ta laboratornoi meditsini, 2 (8), 12–16.
- Voloshina, I. S. (2017). Morfometricheskie izmeneniia semennikov i epididimisov krys posle ingaliatsionnogo vozdeistviia na organizm toluola. Kurskii nauchno-prakticheskii vestnik «Chelovek i ego zdorove», 1, 73–77.
- Guskova, T. A. (2003). Toksikologiia lekarstvennykh sredstv. Moscow: Izdatelskii dom «Russkii vrach», 154.
- Korshunov, M. N., Korshunova, E. S. (2016). Estimation of changes in spermogram and index of sperm DNA fragmentation in patients with premature ejaculation receiving Neurodoz biocomplex. Andrology and Genital Surgery, 17 (2), 119–122. doi:10.17650/2070-9781-2016-17-2-119-122
- Kim, Y.-B., Cheon, Y.-P., Choi, D., Lee, S.-H. (2019). Adverse Effect of Nonylphenol on the Reproductive System in F2 Male Mice : A Qualitative Change? Development & Reproduction, 23 (3), 255–262. doi: http://doi.org/10.12717/dr.2019.23.3.255
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2020 Nadiia Kononenko, Andrey Minukhin, Valentyna Chikitkina
This work is licensed under a Creative Commons Attribution 4.0 International License.
Our journal abides by the Creative Commons CC BY copyright rights and permissions for open access journals.
Authors, who are published in this journal, agree to the following conditions:
1. The authors reserve the right to authorship of the work and pass the first publication right of this work to the journal under the terms of a Creative Commons CC BY, which allows others to freely distribute the published research with the obligatory reference to the authors of the original work and the first publication of the work in this journal.
2. The authors have the right to conclude separate supplement agreements that relate to non-exclusive work distribution in the form in which it has been published by the journal (for example, to upload the work to the online storage of the journal or publish it as part of a monograph), provided that the reference to the first publication of the work in this journal is included.
