The cytokine system’s status in bacterial dysbiosis and bacterial vaginosis

Authors

DOI:

https://doi.org/10.15587/2519-4798.2020.204094

Keywords:

bacterial vaginosis, bacterial dysbiosis, normocenosis, interleukin-1β, interleukin-6, interleukin-8, tumour necrosis factor-α, interleukin-4, interleukin-10, transforming growth factor-1β

Abstract

Interrelations of conditionally-pathogenic microflora and vaginal mucosa’s APC are realized by forming of proinflammatory and regulatory cytokines which can provoke bacterial dysbiosis progression and bacterial vaginosis development.

The aim of the study: to determine cytokine system’s status in the blood and vaginal fluid in bacterial dysbiosis and bacterial vaginosis.

Material and methods. There were used data from 298 women, divided into groups according to the index of conditionally pathogenic microflora and normobiota index: normocenosis (n=53); grade I bacterial dysbiosis (n=128); and grade II bacterial dysbiosis (n=117). In the last group 83 patients with normobiota index >1 lg GE/sample, with bacterial vaginosis isolated. The posterolateral vaginal paries epithelium scrapings examined with PCR: facultative and obligate anaerobes, myco- and ureplasms and yeast fungi were quantified. Contents of IL in VF and in the serum were studied with enzyme-linked immunosorbent assay. For statistical analysis Statistica 10 soft (StatSoft, Inc., USA) was applied.

Results. Blood interleukins’ contents increased with progressing of bacterial dysbiosis, and was maximal in bacterial vaginosis: 3,0-6,0 times (p<0,001) more than in normocenosis. Levels of these interleukins were: interleukin-1β > interleukin-6>interleukin-8>tumour necrosis factor-α > interleukin-2; in vaginal fluid: interleukin-6> tumour necrosis factor-α > interleukin-1β>interleukin-8> interleukin2. Content of γ-interferon in blood and vaginal fluid was higher in bacterial dysbiosis, and was less in manifested bacterial dysbiosis and bacterial vaginosis (comparing to normocenosis). Interleukin-4 and interleukin-10 levels were less in the blood and vaginal fluid along to the progressing of bacterial dysbiosis. Transforming growth factor-1β level in the blood was more in bacterial vaginosis only, whereas in vaginal fluid –in bacterial dysbiosis and bacterial vaginosis. Among blood cytokines interleukin-1β levels correlated with index of conditionally-pathogenic microflora: its content more 24,6 pg/ml indicated bacterial dysbiosis-II, from 9,6 to 24,5 pg/ml –bacterial dysbiosis-I, and contents less than 9,6 pg/ml – normocenosis. Transforming growth factor-1β and interleukin-10 contents in vaginal fluid were suppressive.

Conclusion. Obtained data confirmed determining role of cytokine system in bacterial dysbiosis progression and bacterial vaginosis development. Content of proinflammatory cytokines in the bloodstream increased with progressing of dysbiosis and reached maximum I bacterial vaginosis. Content of anti-inflammatory cytokines with progressing of dysbiosis decreased both in the bloodstream and vaginal fluid

Author Biography

Oleksandr Hruzevskyi, Odessa National Medical University Valikhovskyi lane, 2, Odesa, Ukraine, 65029

PhD, Associate Professor, Head of Department

Departmentof Microbiology, Virology and Immunology

References

  1. Nasioudis, D., Linhares, I., Ledger, W., Witkin, S. (2016). Bacterial vaginosis: a critical analysis of current knowledge. BJOG: An International Journal of Obstetrics & Gynaecology, 124 (1), 61–69. doi: http://doi.org/10.1111/1471-0528.14209
  2. Coudray, M. S., Madhivanan, P. (2020). Bacterial vaginosis—A brief synopsis of the literature. European Journal of Obstetrics & Gynecology and Reproductive Biology, 245, 143–148. doi: http://doi.org/10.1016/j.ejogrb.2019.12.035
  3. Muzny, C. A., Taylor, C. M., Swords, W. E., Tamhane, A., Chattopadhyay, D., Cerca, N., Schwebke, J. R. (2019). An Updated Conceptual Model on the Pathogenesis of Bacterial Vaginosis. The Journal of Infectious Diseases, 220 (9), 1399–1405. doi: http://doi.org/10.1093/infdis/jiz342
  4. Muzny, C. A., Schwebke, J. R. (2016). Pathogenesis of Bacterial Vaginosis: Discussion of Current Hypotheses. Journal of Infectious Diseases, 214 (1), S1–S5. doi: http://doi.org/10.1093/infdis/jiw121
  5. Cox, C., Watt, A. P., McKenna, J. P., Coyle, P. V. (2016). Mycoplasma hominis and Gardnerella vaginalis display a significant synergistic relationship in bacterial vaginosis. European Journal of Clinical Microbiology & Infectious Diseases, 35 (3), 481–487. doi: http://doi.org/10.1007/s10096-015-2564-x
  6. Bertran, T., Brachet, P., Vareille-Delarbre, M., Falenta, J., Dosgilbert, A., Vasson, M.-P. et. al. (2016). Slight Pro-Inflammatory Immunomodulation Properties of Dendritic Cells byGardnerella vaginalis: The “Invisible Man” of Bacterial Vaginosis? Journal of Immunology Research, 2016, 1–13. doi: http://doi.org/10.1155/2016/9747480
  7. Van Teijlingen, N. H., Helgers, L. C., Zijlstra - Willems, E. M., van Hamme, J. L., Ribeiro, C. M. S., Strijbis, K., Geijtenbeek, T. B. H. (2020). Vaginal dysbiosis associated-bacteria Megasphaera elsdenii and Prevotella timonensis induce immune activation via dendritic cells. Journal of Reproductive Immunology, 138, 103085. doi: http://doi.org/10.1016/j.jri.2020.103085
  8. Larsen, J. M. (2017). The immune response toPrevotellabacteria in chronic inflammatory disease. Immunology, 151 (4), 363–374. doi: http://doi.org/10.1111/imm.12760
  9. Anahtar, M. N., Byrne, E. H., Doherty, K. E., Bowman, B. A., Yamamoto, H. S., Soumillon, M. et. al. (2015). Cervicovaginal Bacteria Are a Major Modulator of Host Inflammatory Responses in the Female Genital Tract. Immunity, 42 (5), 965–976. doi: http://doi.org/10.1016/j.immuni.2015.04.019
  10. Hilbert, D. W., Smith, W. L., Paulish-Miller, T. E., Chadwick, S. G., Toner, G., Mordechai, E. et. al. (2016). Utilization of molecular methods to identify prognostic markers for recurrent bacterial vaginosis. Diagnostic Microbiology and Infectious Disease, 86 (2), 231–242. doi: http://doi.org/10.1016/j.diagmicrobio.2016.07.003
  11. Onderdonk, A. B., Delaney, M. L., Fichorova, R. N. (2016). The Human Microbiome during Bacterial Vaginosis. Clinical Microbiology Reviews, 29 (2), 223–238. doi: http://doi.org/10.1128/cmr.00075-15
  12. Masson, L., Barnabas, S., Deese, J., Lennard, K., Dabee, S., Gamieldien, H. et. al. (2018). Inflammatory cytokine biomarkers of asymptomatic sexually transmitted infections and vaginal dysbiosis: a multicentre validation study. Sexually Transmitted Infections, 95 (1), 5–12. doi: http://doi.org/10.1136/sextrans-2017-053506
  13. Kremleva, E. A., Sgibnev, A. V. (2016). Proinflammatory Cytokines as Regulators of Vaginal Microbiota. Bulletin of Experimental Biology and Medicine, 162 (1), 75–78. doi: http://doi.org/10.1007/s10517-016-3549-1
  14. Lipova, E. V., Boldyreva, M. N., Trofimov, D. Iu., Vitvickaia, Iu. G. (2009). Urogenitalnye infekcii, obuslovlennye uslovno-patogennoi biotoi u zhenschin reproduktivnogo vozrasta (kliniko-laboratornaia diagnostika). Moscow, 30.
  15. Gruzevskyy, O. A., Vladimirova, M. P. (2014). The results of complex bacteriological examination of vaginal secretion in bacterial vaginosis. Dosiahnennia biolohii ta medytsyny, 2, 54–57.
  16. Gruzevsky, A. A. (2017). Colonization resistance in vaginal disbioosis: state of humoral and cellular components of immune system. Vestnyk morskoi medytsyni, 4 (77), 103–107.
  17. Delves, P. J., Martin, S. J., Burton, D. R., Roitt, I. M. (2016). Roitt's Essential Immunology. Wiley-Blackwell, 576.
  18. Hurianov, Ya. H., Liakh, Yu. Ye., Parii, V. D., Korotkyi, O. V., Chalyi, O. V., Chalyi, K. O., Tsekhmister, Ya. V. (2018). Posibnyk z biostatystyky. Analiz rezultativ medychnykh doslidzhen u paketi EZR (R-STATISTICS). Kyiv: Vistka, 208.

Published

2020-05-31

How to Cite

Hruzevskyi, O. (2020). The cytokine system’s status in bacterial dysbiosis and bacterial vaginosis. ScienceRise: Medical Science, (3 (36), 50–56. https://doi.org/10.15587/2519-4798.2020.204094

Issue

Section

Medical Science