Study of modification of magnetically labeled yeasts Saccharomyces cerevisiae for copper cations Cu2+ removal

Authors

DOI:

https://doi.org/10.15587/2312-8372.2017.93766

Keywords:

magnetically labeled yeast S. cerevisiae, sorption capacity of biosorbent, functional groups

Abstract

Research of biosorption and search for the cheapest and effective biosorbents of heavy metals are important for wastewater treatment, recovery and allocation of precious metals. Biosorbent artificially provided with magnetic properties quickly and efficiently can be removed from the workspace. Magnetically labeled biosorbent obtained by multi-vortical MHD stirring of yeast S. cerevisiae with nanoscale magnetic labels is able to remove from solutions a wide range of metals, and is the subject of the study.

Sorption properties of cell walls in the case of passive biosorption are dependent from represented on its surface functional groups such as carboxyl and amino groups. Quantitative analysis of the contribution of functional groups, lipids and proteins in sorption capacity of magnetically labeled cells of interest for understanding the sorption of metal cations, interactions of particles of magnetite with cell wall and sorption of metal cations by immobilized magnetite. There is a need to detect how many functional groups are blocked by magnetite during multi-vortical MHD stirring.

To solve this problem it is prompted to investigate and analyze the sorption capacity of magnetically labeled yeast by modifying the surface of biosorbent by extraction or blocking in terms of biosorption by functional groups.

The results showed that the carboxyl groups, and after them the amino group of the cell wall of native and magnetically labeled yeasts have the greatest contribution to the sorption of copper cations. Magnetic labels interact with −COOH groups and block them − about 15 % of the cell wall components extracted using NaOH. At the same time 1 % by weight of magnetite provides biosorbent equivalent amount of copper cations binding sites on the surface of cells, which in turn leads to the same sorption capacity of native and magnetically labeled yeasts.

Author Biography

Yuriy Karpenko, National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute», Peremohy ave., 37, Kyiv, Ukraine, 03056

Postgraduate student

Department of Bioinformatics

References

  1. Gorobets, S. V., Mykhailenko, N. O., Karpenko, Yu. V. (2013). Determination of optimum characteristics of magnetically operated biosorbent based on Saсcharomyces cerevisiae yeasts. Chemistry, physics and technology of surface, 4 (2), 219–225.
  2. Wang, J., Chen, C. (2009). Biosorbents for heavy metals removal and their future. Biotechnology Advances, 27 (2), 195–226. doi:10.1016/j.biotechadv.2008.11.002
  3. Gorobets, S. V., Karpenko, Yu. V., Kovalev, O. V., Olishevsky, V. V. (2013). Application of Magnetically Labeled Cells S. cerevisiae as Biosorbents at Treatment Plants. Naukovi Visti NTUU KPI, 3 (89), 42–47.
  4. Abbas, S. H., Ismail, I. M., Mostafa, T. M., Sulaymon, A. H. (2014). Biosorption of Heavy Metals: A Review. Journal of Chemical Science and Technology, 3 (4), 74–102.
  5. Wang, T., Zheng, X., Wang, X., Lu, X., Shen, Y. (2017). Different biosorption mechanisms of Uranium(VI) by live and heat-killed Saccharomyces cerevisiae under environmentally relevant conditions. Journal of Environmental Radioactivity, 167, 92–99. doi:10.1016/j.jenvrad.2016.11.018
  6. Geva, P., Kahta, R., Nakonechny, F., Aronov, S., Nisnevitch, M. (2016). Increased copper bioremediation ability of new transgenic and adapted Saccharomyces cerevisiae strains. Environmental Science and Pollution Research, 23 (19), 19613–19625. doi:10.1007/s11356-016-7157-4
  7. Xu, M., Zhang, Y., Zhang, Z., Shen, Y., Zhao, M., Pan, G. (2011). Study on the adsorption of Ca2+, Cd2+ and Pb2+ by magnetic Fe3O4 yeast treated with EDTA dianhydride. Chemical Engineering Journal, 168 (2), 737–745. doi:10.1016/j.cej.2011.01.069
  8. Emanet, M., Fakhrullin, R., Çulha, M. (2016). Boron Nitride Nanotubes and Layer-By-Layer Polyelectrolyte Coating for Yeast Cell Surface Engineering. ChemNanoMat, 2 (5), 426–429. doi:10.1002/cnma.201600044
  9. Gorobets, S. V., Gorobets, O. Y., Goyko, I. Y., Kasatkina, T. P. (2004). Intensification of the extraction process of copper and chromium (VI) ions from the solutions in a magnetic field. Functional Materials, 11 (4), 793–797.
  10. Aronbaev, S. D., Nasimov, A. M., Aronbaev, D. M. (2011). Biosorbtsiia tiazhelyh metalov kletochnymi obolochkami drozhzhei saccharomyces cerevisiae. Vserossiiskii zhurnal nauchnyh publikatsii, 1 (2), 13–15.
  11. Gorobets, S. V., Gorobets, O. Yu., Demianenko, I. V., Nikolaenko, R. N. (2013). Self-organization of magnetite nanoparticles in providing Saccharomyces cerevisiae Yeasts with magnetic properties. Journal of Magnetism and Magnetic Materials, 337-338, 53–57. doi:10.1016/j.jmmm.2013.01.004
  12. Gorobets, S., Gorobets, O., Chyzh, Y., Kovalev, O., Perizhok, V., Golub, V. (2016). Analysis of effectivness of magnetically labeled biosorbent obtained through the mechanical and magnetohydrodynamic stirring. EUREKA: Physics and Engineering, 5, 37–43. doi:10.21303/2461-4262.2016.00165
  13. Liu, J., Zhao, Z., Jiang, G. (2008). Coating Fe3O4Magnetic Nanoparticles with Humic Acid for High Efficient Removal of Heavy Metals in Water. Environmental Science & Technology, 42 (18), 6949–6954. doi:10.1021/es800924c
  14. Soares, E. V., Soares, H. M. V. M. (2013). Cleanup of industrial effluents containing heavy metals: a new opportunity of valorising the biomass produced by brewing industry. Applied Microbiology and Biotechnology, 97 (15), 6667–6675. doi:10.1007/s00253-013-5063-y
  15. Kushnirov, V. V. (2000). Rapid and reliable protein extraction from yeast. Yeast, 16 (9), 857–860. doi:10.1002/1097-0061(20000630)16:9<857::aid-yea561>3.0.co;2-b
  16. Tobin, J. M., Cooper, D. G., Neufeld, R. J. (1990). Investigation of the mechanism of metal uptake by denatured Rhizopus arrhizus biomass. Enzyme and Microbial Technology, 12 (8), 591–595. doi:10.1016/0141-0229(90)90132-a
  17. Kapoor, A., Viraraghavan, T. (1997). Heavy metal biosorption sites in Aspergillus niger. Bioresource Technology, 61 (3), 221–227. doi:10.1016/s0960-8524(97)00055-2
  18. Azevedo, R. B., Silva, L. P., Lemos, A. P. C., Bao, S. N., Lacava, Z. G. M., Safarik, I., Safarikova, M., Morais, P. C. (2003). Morphological study of saccharomyces cerevisiae cells treated with magnetic fluid. IEEE Transactions on Magnetics, 39 (5), 2660–2662. doi:10.1109/tmag.2003.815547

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Published

2017-01-31

How to Cite

Karpenko, Y. (2017). Study of modification of magnetically labeled yeasts Saccharomyces cerevisiae for copper cations Cu2+ removal. Technology Audit and Production Reserves, 1(3(33), 45–49. https://doi.org/10.15587/2312-8372.2017.93766

Issue

Vol. 1 No. 3(33) (2017): Chemical Engineering

Section

Ecology and Environmental Technology: Original Research

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Copyright (c) 2017 Yuriy Karpenko

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