DOI: https://doi.org/10.26641/2307-5279.23.1.2019.161659

Correction of thiol status in blood and tissues of kidney of rats in the design of acute pyelonephritis and concomitant diabetes mellitus type II

S.A. Borisov, F.I. Kostyev, A.V. Borisov

Abstract


The conducted research is experimental-clinical, conducted in laboratory rats and is devoted to the determination of the effectiveness of correction of thiol status in blood and tissues of kidney of rats in acute pyelonephritis and concomitant streptozotocin-type diabetes mellitus type II.

The use of multi-vector drugs in the modeling of acute pyelonephritis with concomitant type 2 diabetes caused a likely increase in the level of reduced glutathione in the blood and kidneys and decreased oxidized in relation to the group with the traditional medication effect. Recovery of thiol status in blood and kidneys of rats in this pathology due to the use of multi-vector drugs will help to normalize the functional capacity of the kidneys. The pathogenetically important role of concomitant type 2 diabetes mellitus in a significant violation of the thiol status in blood plasma and kidney tissues of animals with acute pyelonephritis was established, which showed a decrease in the level of an important component of the antioxidant and detoxification system - reduced glutathione.

Keywords


acute pyelonephritis; thiol status; oxidative stress; diabetes mellitus; flow; medication correction; detoxification

References


Ефремова Е.Д., Ботвинева Л.А. Сахарный диабет: современные достижения и перспективные направления в профилактике и лечении. Материалы Всерос. науч. -практич. конф., посвященной 60-летию ФГБУ СКФНКЦ ФМБА России. – Пятигорск: РИА-КМВ, 2017. – С. 77–86.

Omer Evirgen, Ahmet Gцkзe, Oktay Hasan Ozturk et al. Effect of Thymoquinone on Oxidative Stress in Escherichia coli – Induced Pyelonephritis in Rats // Current Therapeutic Research. – 2011. – V. 72, N 5. – Р. 204–215.

Emin Ozbek. Induction of Oxidative Stress in Kidney // International Journal of Nephrology. –2012, Article ID 465897, 9 pages. doi:10.1155/2012/465897.

Nasri H. Antioxidant therapy to ameliorate chronic kidney disease induced by oxidative stress; an updated mini-review. J. Prev. Epidemiol. 2017; 2(1): e04.

Drachuk V. M., Zamorskii I. I., Goroshko O. M. The influence of glutathione on the prooxidant-antioxidant activity in kidneys and blood of rats with rhabdomyolytic model of acute kidney injury // Укр. біофарм. журн. 2016;4(45);20–123.

Franco R. , Cidlowski J. A. Apoptosis and Glutathione: Beyond an Antioxidant // Cell Death and Dif ferentiation. – 2009. – V. 16. – Р. 1303–1314.

Derai El-hadjela, Kechrid Zine, Bouhafs Leila. The beneficial effect of combined administration of vitamins C and E on renal function and selected parameters of antioxidant system in diabetic rats fed zinc-deficient diet // Afr. J. Biotechnol. – 2013. – Vol. 12(43). – Р. 6240–6232.

Yumi Takiyama, Masakazu Haneda. Hypoxia in Diabetic Kidneys // BioMed Research International. – 2014. –Article ID 837421, 10 pages. doi:10.1155/2014/837421.

Bergmeyer H.U. Metoden der enzymatischen Analyse / H.U. Bergmeyer. – Berlin: Akademie- Verlag, 1970. – 2220 p.