Microbiota of the rhizosphere of winter wheat under the action of biopraparation Azotohelp and Groundfix

Authors

  • D. Yakovenko Institute of Agroecology and Environmental Management of NAAS; BTU Biotech Company, Ukraine
  • V. Boroday Institute of Agroecology and Environmental Management of NAAS; National University of Life and Environmental Sciences of Ukraine, Ukraine https://orcid.org/0000-0002-8787-8646

DOI:

https://doi.org/10.33730/2310-4678.4.2025.346195

Keywords:

Triticum aestivum L., rhizosphere, agrocenoses, microorganisms, phytopathogens, antagonists, microbial preparations

Abstract

The study investigated changes in the abundance and structure of the rhizosphere mycobiota of winter wheat using different methods of applying the multifunctional biological products Groundfix® and Azotohelp®. The research was conducted in the experimental field of the Khmelnytskyi State Agricultural Research Station of the Institute of Feed and Agriculture of Podillia (SARS IFAP). The most favourable ratio of saprotrophic and pathogenic fungi in the BBCH 61–69 development phase of winter wheat was formed in the variant with the combined application of Groundfix (1.5 l/ha) + Azotohelp (1.5 l/ha). In this variant, the proportion of phytopathogens was the lowest (4 thousand CFU/g of soil), which was 4.6 times less than in the control variant, and the proportion of saprotrophic mycobiota was 94.7%, in the other variants with separate application of biological products — 64.7–85.8%, in the control variant — 63.6%. The treatment with Azotohelp (1.5 l/t) + foliar spraying with Azotohelp (0.5 l/ha) in the spring tillering phase was close in enhancing soil biological activity to this variant in terms of effectiveness. The largest number of antagonistic fungi of the genera Trichoderma and Gliocladium in the rhizosphere of wheat was observed applying biological products during pre-sowing cultivation. The use of Azotohelp (3 l/ha) for pre-sowing cultivation led to an increase in the proportion of the genus Trichoderma by 60% compared to 5.6–29.4% in other variants, which indicates the formation of a high level of fungistatic activity of the soil and affected the suppression of pathogens both in the flowering phase (3.6 thousand CFU/g of soil compared to 7.3–18.4 thousand CFU/g of soil in other variants) and in the development phase of BBCH 83–89 (3.8 thousand CFU/g of soil versus 7.8–27.0 thousand CFU/g of soil). The effectiveness of this variant during the wheat growing season in 2021 was lower, which can be explained by unfavourable hydrothermal conditions.

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Published

2025-11-14

Issue

No. 4 (2025)

Section

Articles

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