THE QUESTION ABOUT β-GLUCAN SUPRAMOLECULAR STRUCTURES OF YEAST SACCHAROMYCES CEREVISIAE
DOI:
https://doi.org/10.15673/2073-8684.4/2015.55867Keywords:
yeast Saccharomyces cerevisiae, β-glucan, supramolecular structure, water-soluble fragments, immunomodulatorAbstract
The biological activity of β-glucan depends on several factors: polysaccharide water solubility, its molecular mass, type and configuration of glycoside bonds between residual monosaccharides, and macromolecular branching and conformations.
The article shows that structure of the baker's yeast (Saccharomyces cerevisiae) glucan, isolated with peroxide method, contains sections with varying order strength. Hydrogen peroxide (H2O2) yeast treatment is supplemented by the destruction of amorphous formations and increase of glucan preparations crystallinity degree in 2.6...3.3 times compared to the reference specimen. It has been shown that water-soluble glucan has more ordered supramolecular structure (crystallinity index is 4.06) than the original insoluble glucan (crystallinity index is 2.55). The water-soluble glucan preparation obtained by partial enzimatic degradation of structural β-glucan contains molecular formations in the form of triple helices.
Animal experiments showed the effectiveness of obtained β-glucan preparations as immunomodulators. The aggregate research findings of preparation supramolecular structure and its medical and biological assessment would be an argument, confirming the relation of β-glucan’s biological action to triple helix in its structure.
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