Development of endogenous intoxication and changes in the concentration of C-reactive protein in the blood in bacterial-immunoid periodontitis on the background of the use of prosthetic bases

A.Ye. Demkovych; Y.I. Poliukhovych; L.V. Piasetska; S.O. Rosolovska

doi:10.26641/2307-0404.2025.4.348272

Authors

A.Ye. Demkovych I. Horbachevsky Ternopil National Medical University, Maidan Voli, 1, Ternopil, 46001, Ukraine https://orcid.org/0000-0001-9823-4283
Y.I. Poliukhovych I. Horbachevsky Ternopil National Medical University, Maidan Voli, 1, Ternopil, 46001, Ukraine https://orcid.org/0009-0007-4726-9372
L.V. Piasetska I. Horbachevsky Ternopil National Medical University, Maidan Voli, 1, Ternopil, 46001, Ukraine https://orcid.org/0000-0002-5640-2258
S.O. Rosolovska I. Horbachevsky Ternopil National Medical University, Maidan Voli, 1, Ternopil, 46001, Ukraine https://orcid.org/0000-0003-4768-3905

DOI:

https://doi.org/10.26641/2307-0404.2025.4.348272

Keywords:

removable prosthetics, base materials, nylon prosthesis, acrylic prosthesis, periodontium, periodontitis, endogenous intoxication, C-reactive protein

Abstract

The purpose of this work was to determine changes in endogenous intoxication indicators and C-reactive protein levels in experimental bacterial-immune periodontitis under the conditions of using acrylic and nylon bases of removable dentures. Experimental studies were conducted on clinically healthy male white rats weighing 150-200 g, which were kept in conditions that met the requirements of sanitation and good laboratory practice (GLP). Acrylic and nylon dentures were made for rats, and bacterial-immune periodontitis was simulated by injecting Staphylococcus aureus and Streptococcus hemolyticus in egg protein into periodontal tissue. The level of endogenous intoxication was assessed by the erythrocyte index, the middle molecular weight molecules were determined by spectrophotometer, and the level of C-reactive protein was determined by enzyme-linked immunosorbent assay. The data were processed using nonparametric statistical methods in STATISTICA 10.0. Characterizing the indicators of the erythrocyte intoxication index of the experimental model of periodontitis and against the background of prosthetics with nylon bases, it should be noted that the results obtained were higher (by 1.27 times; (p<0.001) than the control indicators, but lower than the data of animals without prosthetics (by 1.44 times; p<0.001) and with acrylic prostheses (by 1.24 times; p<0.001), respectively. The content of C-reactive protein after fixation of acrylic bases was significantly increased compared to the intact group (by 2.50 times; p<0.001). The obtained data also showed that the level of this protein was by 1.60 times higher (p<0.001) compared to the indicators in rats with bacterial-immune periodontitis without the use of removable acrylic structures. In the blood serum of rats with inflammatory periodontal disease and prosthetics with nylon bases, the level of C-reactive protein also increased significantly: it was by 1.91 times higher (p<0.001) compared to the control group and by 1.22 times (p<0.01) compared to animals with periodontitis on 30^th day without dentures. The use of different types of prosthetic bases in bacterial-immune inflammation of the periodontium affects the level of endogenous intoxication, reducing the dynamics of the inflammatory process in the periodontal complex. However, the use of acrylic bases contributes to an increase in the level of C-reactive protein, which indicates a more negative effect of acrylic plastics on the main mechanisms of inflammation in periodontal tissues.

References

Valgimigli L. Lipid Peroxidation and Antioxidant Protection. Biomolecules. 2023 Aug 24;13(9):1291. doi: https://doi.org/10.3390/biom13091291

Demkovych A, Bondarenko Y, Hasiuk PA. Oxidative modification of proteins in the process of experimental periodontitis development. Interv Med Appl Sci. 2017 Dec;9(4):218-221. doi: https://doi.org/10.1556/1646.9.2017.28

Abdulkareem AA, Al-Taweel FB, Al-Sharqi AJB, Gul SS, Sha A, Chapple ILC. Current concepts in the pathogenesis of periodontitis: from symbiosis to dysbiosis. J Oral Microbiol. 2023 Apr 2;15(1):2197779. doi: https://doi.org/10.1080/20002297.2023.2197779

Syniachenko OV, Iermolaieva MV, Aliieva NYu, Liventsova KV, Verzilov SM, Syniachenko TY. [Clinical-pathogenetic significance of msddle mass molecules of different fractions in pulmonary expirates of patients with rheumatoid arthritis]. Tuberculosis Lung Diseases HIV Infection. 2021;(1):60-6. Ukrainian. doi: https://doi.org/10.30978/TB2021-1-60

Konovchuk V, Andrushchak A, Kushnir S, Maksymiuk V, Kokalko M. The state of toxin-releasing function of the kidneys in the syndrome of endogenous intoxication of purulent-septic origin in patients with diabetes mellitus. Wiad Lek. 2022;75(7):1724-7. doi: https://doi.org/10.36740/WLek202207121

Herwald H, Egesten A. C-Reactive Protein: More than a Biomarker. J Innate Immun. 2021;13(5):257-8. doi: https://doi.org/10.1159/000519091

Lokes K, Lychman V, Steblovsky D, Bondarenko V, Avetikov D. Dynamics of changes of c-reactive protein in the serum content in patients with phlegmons of the jaws and facial localization at different terms of the drug. World of Medicine and Biology. 2021;3(77):97-101. doi: https://doi.org/10.26724/2079-8334-2021-3-77-101

Shcherba V, Demkovych A, Hasiuk P, Lebid O, Duda K, Stoikevych H. Morphological changes of perio-dontal components under experimental lipopolysaccharide periodontitis combined with hyperthyroidism. Wiadomosci Lekarskie. 2022;8(2):1960-4. doi: https://doi.org/10.36740/WLek202208203

Gorodova-Andreeva TV, Liakhovsky VI, Sydo-renko AV. [Role of c-reactive protein in assessing efficiency of treatment of purulent wounds in soft tissues]. Actual Problems of the Modern Medicine: Bulletin of Ukrainian Medical Stomatological Academy. 2022;22(3-4):38-41. Ukrainian.

doi: https://doi.org/10.31718/2077-1096.22.3.4.38

Nidzelsky M, Tsvetkova N. [Dynamics of sound production during the period of adaption to full removable dentures]. Actual Problems of the Modern Medicine: Bulletin of Ukrainian Medical Stomatological Academy. 2020;20(3):87-91. Ukrainian. doi: https://doi.org/10.31718/2077-1096.20.3.87

Lokota YuYe, Paliichuk IV. Dynamics of indicators of microbiocenosis of the oral cavity in treatment of patients with complete removable plate prosthesis with the use of ozone therapy. Art of Medicine. 2022;2(22):53-62. doi: https://doi.org/10.21802/artm.2022.2.22.53

Pires JR, Nogueira MRS, Nunes AJF, et al. Depo-sition of Immune Complexes in Gingival Tissues in the Presence of Periodontitis and Systemic Lupus Erythe-matosus. Front Immunol. 2021;12:591236. doi: https://doi.org/10.3389/fimmu.2021.591236

Chaulagain R, Thakur SN, Khanal B, Sapkota SM, Chaudhary NK. Microbial Spectrum of Complete Denture Wearer in Old Age People of Chitwan. J Nepal Health Res Counc. 2023 Sep 10;21(1):122-8. doi: https://doi.org/10.33314/jnhrc.v21i1.4603

Makeev V, Skalskyi V, Huniovskyi Ya, Huniov-ska R. [Static crack resistance evaluation of polymeric materials for the manufacture of bases for removable dentures]. Actual Dentistry. 2019;(2):102-6. Ukrainian. doi: https://doi.org/10.33295/1992-576X-2019-2-102

Bolon B, et al. Good laboratory practice in the academic setting: Fundamental principles for nonclinical safety assessment and GLP-compliant pathology support when developing innovative biomedical products. ILAR Journal. 2018;59:18-28. doi: https://doi.org/10.1093/ilar/ily008

Kostić M, Igić M, Gligorijević N, Nikolić V, Stošić N, Nikolić L. The Use of Acrylate Polymers in Dentistry. Polymers (Basel). 2022 Oct 25;14(21):4511. doi: https://doi.org/10.3390/polym14214511

Le Bars P, Bandiaky ON, Le Guéhennec L, Clouet R, Kouadio AA. Different Polymers for the Base of Removable Dentures? Part I: A Narrative Review of Mechanical and Physical Properties. Polymers (Basel). 2023 Aug 22;15(17):3495. doi: https://doi.org/10.3390/polym15173495

Demkovych A, Rubas L, Luchynskyi V, Luchynska Y, Stoikevych H, Machogan V. Changes of ultrastructural organization in periodontal complex components in experimental periodontitis and its correction with quercetin. Pharmacia. 2022;69(2):563-9. doi: https://doi.org/10.3897/pharmacia.69.e82128

Kachur OI, Fira LS, Likhatsky PG. [Endogenic in-toxication in rats with experimental carcinogenesis after application of cytostatics on the background of sorption therapy]. Medical and Clinical Chemistry. 2020;22(2):39-46. Ukrainian.

doi https//doi.org/10.11603/mcch.2410-681X.2020.v.i2.11356

European convention for the protection of verte-brate animal used for experimental and other scientific purposes [Internet]. Council of Europe, Strasbourg. 1986 [cited 2024 Feb 23]. 11 р. Available from: https://rm.coe.int/168007a67b

Zhu X. Sample size calculation for Mann-Whitney U test with five methods. Int J Clin Trials. 2021 Aug;8(3):184-95. doi: https://doi.org/10.18203/2349-3259.ijct20212840

Kim WJ, Soh Y, Heo SM. Recent Advances of Therapeutic Targets for the Treatment of Periodontal Disease. Biomol Ther (Seoul). 2021 May 1;29(3):263-7. doi: https://doi.org/10.4062/biomolther.2021.001

Olekshij PV, Regeda MS. Peculiarities of changes in endogenous intoxication indicators in the dynamics of the experimental periodontitis development. Journal of Education, Health and Sport. 2023;13(2):309-14. doi: https://doi.org/0.12775/JEHS.2023.13.02.044

D'Ambrosio F, Santella B, Di Palo MP, Giorda-no F, Lo Giudice R. Characterization of the Oral Micro-biome in Wearers of Fixed and Removable Implant or Non-Implant-Supported Prostheses in Healthy and Pathological Oral Conditions: A Narrative Review. Microorganisms. 2023;11(4):1041. doi: https://doi.org/10.3390/microorganisms11041041

Rai J, Shah V, Shah M. Periodontitis severity grading scale and C-reactive protein: A possible relation. Cureus. 2023;15(7):e41618. doi: https://doi.org/10.7759/cureus.41618