Development of endogenous intoxication and changes in the concentration of C-reactive protein in the blood in bacterial-immunoid periodontitis on the background of the use of prosthetic bases

Аннотация

The purpose of this work was to determine changes in endogenous intoxication indicators and C-reactive protein levels in experimental bacterial-immune periodontitis under the conditions of using acrylic and nylon bases of removable dentures. Experimental studies were conducted on clinically healthy male white rats weighing 150-200 g, which were kept in conditions that met the requirements of sanitation and good laboratory practice (GLP). Acrylic and nylon dentures were made for rats, and bacterial-immune periodontitis was simulated by injecting Staphylococcus aureus and Streptococcus hemolyticus in egg protein into periodontal tissue. The level of endogenous intoxication was assessed by the erythrocyte index, the middle molecular weight molecules were determined by spectrophotometer, and the level of C-reactive protein was determined by enzyme-linked immunosorbent assay. The data were processed using nonparametric statistical methods in STATISTICA 10.0. Characterizing the indicators of the erythrocyte intoxication index of the experimental model of periodontitis and against the background of prosthetics with nylon bases, it should be noted that the results obtained were higher (by 1.27 times; (p<0.001) than the control indicators, but lower than the data of animals without prosthetics (by 1.44 times; p<0.001) and with acrylic prostheses (by 1.24 times; p<0.001), respectively. The content of C-reactive protein after fixation of acrylic bases was significantly increased compared to the intact group (by 2.50 times; p<0.001). The obtained data also showed that the level of this protein was by 1.60 times higher (p<0.001) compared to the indicators in rats with bacterial-immune periodontitis without the use of removable acrylic structures. In the blood serum of rats with inflammatory periodontal disease and prosthetics with nylon bases, the level of C-reactive protein also increased significantly: it was by 1.91 times higher (p<0.001) compared to the control group and by 1.22 times (p<0.01) compared to animals with periodontitis on 30^th day without dentures. The use of different types of prosthetic bases in bacterial-immune inflammation of the periodontium affects the level of endogenous intoxication, reducing the dynamics of the inflammatory process in the periodontal complex. However, the use of acrylic bases contributes to an increase in the level of C-reactive protein, which indicates a more negative effect of acrylic plastics on the main mechanisms of inflammation in periodontal tissues.