Development of method quantitative content of dihydroquercetin. Report 1
DOI:
https://doi.org/10.15587/2313-8416.2016.59243Keywords:
photocolorimetric method, quantitative determination, validation characteristics, the State Pharmacopoeia of Ukraine, dihydroquercetinAbstract
Today is markedly increasing scientific interest in the study of flavonoids in plant objects due to their high biological activity. In this regard, the urgent task of analytical chemistry is in developing available analytical techniques of determination for flavonoids in plant objects.
Aim. The aim was to develop specific techniques of quantitative determination for dihydroquercetin and determination of its validation characteristics.
Methods. The technique for photocolorimetric quantification of DQW, which was based on the specific reaction of cyanidine chloride formation when added zinc powder to dihydroquercetin solution in an acidic medium has been elaborated.
Results. Photocolorimetric technique of determining flavonoids recalculating on DQW has been developed, its basic validation characteristics have been determined. The obtained metrological characteristics of photocolorimetric technique for determining DQW did not exceed admissibility criteria in accordance with the requirements of the State Pharmacopoeia of Ukraine.
Conclusions. By the results of statistical analysis of experimental data, it has been stated that the developed technique can be used for quantification of DQW. Metrological data obtained indicate that the method reproduced in conditions of two different laboratories with confidence probability 95 % unit value deviation was 101,85±2,54 %
References
Galleano, M., Pechanova, O., G. Fraga, C. (2010). Hypertension, Nitric Oxide, Oxidants, and Dietary Plant Polyphenols. Current Pharmaceutical Biotechnology, 11 (8), 837–848. doi: 10.2174/138920110793262114
Przybylski, R., Gruczyńska, E. (2009). A review of nutritional and nutraceutical components of Buckwheat. Eur. J. Plant Sci. Biotechnol., 3 (1), 10–22. Available at: http://www.globalsciencebooks.info/Online/GSBOnline/images/0906/EJPSB_3(SI1)/EJPSB_3(SI1)10-22o.pdf
Cucciolla, V., Borriello, A., Oliva, A., Galletti, P., Zappia, V., Ragione, F. D. (2007). Resveratrol: From Basic Science to the Clinic. Cell Cycle, 6 (20), 2495–2510. doi: 10.4161/cc.6.20.4815
Zielińska, D., Szawara-Nowak, D., Zieliński, H. (2010). Determination of the antioxidant activity of rutin and its contribution to the antioxidant capacity of diversified buckwheat origin material by updated analytical strategies. Polish journal of food and nutrition sciences, 60 (4), 315–321.
Krikova, A. V. et. al (2006). Biologicheskaja aktivnost' rastitel'nyh istochnikov flavonoidov. Farmacija, 54 (3), 17–18.
Evdokimova, O. V. (2006). Preparaty rastitel'nogo proishozhdenija pri hronicheskoj venoznoj nedostatochnosti. Novaja apteka, 4, 11–12.
Es'kin, A. P., Levdanskij, V. A., Polezhaeva, N. I. (1998). Metod kolichestvennogo fotometricheskogo opredelenija digidrokvercetina. Himija rastitel'nogo syr'ja, 3, 41–46.
Bagdonaitė, E., Mártonfi, P., Repčák, M., Labokas, J. (2012). Variation in concentrations of major bioactive compounds in Hypericum perforatum L. from Lithuania. Industrial Crops and Products, 35 (1), 302–308. doi: 10.1016/j.indcrop.2011.07.018
Liu, B., Zhu, Y. (2007). Extraction of flavonoids from flavonoid-rich parts in tartary buckwheat and identification of the main flavonoids. Journal of Food Engineering, 78 (2), 584–587. doi: 10.1016/j.jfoodeng.2005.11.001
Bobkov, Ju. G., Babajan, Je. A. et. al (1987). Obshhie metody analiza: Gosudarstvennaja farmakopeja SSSR. Issue 1. Moscow: «Medicina», 336.
Vladymyrov, O. Ju. (2015). Rozrobka ta standartyzacija profilaktychnyh zasobiv na osnovi roslynnyh dzherel dygidrokvercetynu. Kharkiv, 24.
Derzhavna Farmakopeja Ukrai'ny (2008). Derzh. p-vo “Naukovo–ekspertnyj farmakopejnyj centr”. Kharkiv: RIREG, 617.
Jevtifjejeva, O. A. (2011). Standartyzacija pidhodiv do ocinky jakosti ekstemporal'nyh likars'kyh zasobiv. Kharkiv, 42.
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Copyright (c) 2016 Олександр Юрійович Владимиров, Вікторія Акоповна Георгіянц, Ганна Олександрівна Бур’ян, Шарк Амжад Ібрагім Абу, Лідія Олександрівна Петрушова, Тетяна Вікторівна Алексєєва, Олександр Анатолійович Здорик, Василь Васильович Гриненко, Ірина Анатоліївна Данилова
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