Research of the colonization level of the fungi Malassezia spp. and Candida spp. on the skin of patients with skin diseases
DOI:
https://doi.org/10.15587/2519-4798.2018.121964Keywords:
Malassezia spp, Candida spp., colonization of the skin, the method of “bacterial swab test”, skin diseasesAbstract
The aim of the work was to research the skin colonization of Malassezia spp., and Candida spp. of the patients with skin diseases and healthy individuals by the method of “bacterial swab test”. The features of colonization of the skin of patients with skin diseases the fungi of the genus Malassezia, Candida and molds, which complicate of these diseases, are established.
Methods of the research. It has included 107 patients with skin diseases and 20 healthy individuals. For the study, sterile plastic Petri dishes (d=3.5 cm.) with Leeming-Notman agar were used. After 5 days of incubation, the numbers of colonies grown on the dish were counted. The identification was performed to the morphological, microscopic and tinctorial properties.
Results: The highest level of colonization (>20 CFU/10 cm2) on the intact skin of the fungi of the genus Malassezia were detected in patients with eczema and atopic dermatitis (18.6 % and 30 %), and in the “locus morbi” in 15 % and 25 % respectively. Candida spp. in quantity >20 CFU were detected in the patients with psoriasis (9.6 %) on “locus morbi”. Malassezia spp. was detected in 40 % healthy individuals, in quantity 10–15 CFU. Candida spp was not detected in healthy individuals.
Conclusions: The method of “bacterial swab test” was used to research the skin colonization of Malassezia spp., and Candida spp. of the patients with skin diseases and healthy individuals
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