Role of circulating oxidized LDL: a useful diagnostic risk marker in cardiovascular disease




acute myocardial infarction (AMI), unstable angina (UA), stable angina pectoris (SAP), cardiovascular disorder (CVD)


The aim: to assess levels of circulating plasma ox-LDL in various subgroups with different CVD and their relationship with oxidative stress markers, MDA, catalase, and traditional coronary disease risk factors.

Material and methods: a total of 215 subjects divided into four groups comprising 54 healthy controls, patients with the SAP were 52 persons, with the UAP 53 ones, and with the AMI there were 56 persons, respectively. Lipid profile parameters (TC, TG, HDL-C, LDL-C, and VLDL-C), plasma MDA, catalase were estimated by kit methods, TBARS method, and colorimetric assay respectively. Plasma Ox-LDL was estimation by competitive ELISA kit method (Mercodia) with the help of specific monoclonal antibody mAb4Eb. Results were present as mean ± SD, significance level at p-values<0.05 with Student’s unpaired t-test. Data analysis was performed by software package SPSS version 17.0.

Results: it showed a highly significant (p<0.001) correlation in SAP, UAP, and AMI except for age in the SAP subgroup, moderately significant (p<0.01). Lipid profile except HDL-C was found highly elevated (p<0.001) in subgroups than in healthy controls. HDL-C was higher (p<0.001) in controls with respect to patient subgroups. Comparison of oxidative stress markers (MDA and catalase) and ox-LDH in control with patient’s subgroup indicates highly significant (p<0.001) correlation. The correlation between SAP & UAP was insignificant (p<0.05), SAP with AMI was significant (p<0.05), and UAP & AMI were highly significant (p<0.001). Large interquartile range in SAP subgroup suggesting scattered deviation in the mean value as compared to UAP and AMI showed in the box and whiskers plot and concluded that significantly elevated level of ox-LDL in SAP, UAP, and AMI subgroups indicate its diagnostic importance of CVD.

Conclusions: study concluded that significantly elevated level of ox-LDL in SAP, UAP, and AMI subgroups indicate its diagnostic importance of CVD.

Author Biographies

Deepti Mandsorwale, Shaikh-Ul-Hind Maulana Mahmood Hasan Medical College

PhD, Assistant Professor

Department of Biochemistry

Bindu Sharma, Varun Arjun Medial College & Rohilkhand Hospital

PhD, Associate Professor

Department of Biochemistry

Munindra Pratap Singh, Gajara Raja Medical College

MD, Demonstrator

Department of Physiology


  1. Benjamin, E. J., Muntner, P., Alonso, A., Bittencourt, M. S., Callaway, C. W. et. al. (2019). Heart Disease and Stroke Statistics – 2019 Update: A Report From the American Heart Association. Circulation, 139 (10), e56–e528. doi:
  2. Holvoet, P. (2012). Stress in Obesity and Associated Metabolic and Cardiovascular Disorders. Scientifica, 2012, 1–19. doi:
  3. Prabhakaran, D., Yusuf, S. (2010). Cardiovascular disease in India: learnt & challenges ahead. Indian Journal of Medical Research, 132 (5), 529–530.
  4. Yang, X., Li, Y., Li, Y., Ren, X., Zhang, X., Hu, D. et. al. (2017). Oxidative Stress-Mediated Atherosclerosis: Mechanisms and Therapies. Frontiers in Physiology, 8. doi:
  5. Di Pietro, N., Formoso, G., Pandolfi, A. (2016). Physiology and pathophysiology of oxLDL uptake by vascular wall cells in atherosclerosis. Vascular Pharmacology, 84, 1–7. doi:
  6. Linton, M. R. F., Yancey, P. G., Davies, S. S., Jerome, W. G., Linton, E. F., Song, W. L. et. al.; Feingold, K. R., Anawalt, B., Boyce, A., Chrousos, G., de Herder, W. W., Dungan, K. et. al. (Eds.) (2019). The role of lipids and lipoproteins in atherosclerosis. in Endotext. South Dartmouth: MD, Inc. Available at:
  7. Ndrepepa, G., Braun, S., Mehilli, J., Von Beckerath, N., Schömig, A., Kastrati, A. (2008). Myeloperoxidase level in patients with stable coronary artery disease and acute coronary syndromes. European Journal of Clinical Investigation, 38 (2), 90–96. doi:
  8. Gao, S., Liu, J. (2017). Association between circulating oxidized low-density lipoprotein and atherosclerotic cardiovascular disease. Chronic Diseases and Translational Medicine, 3 (2), 89–94. doi:
  9. Heslop, C. L., Frohlich, J. J., Hill, J. S. (2010). Myeloperoxidase and C-Reactive Protein Have Combined Utility for Long-Term Prediction of Cardiovascular Mortality After Coronary Angiography. Journal of the American College of Cardiology, 55 (11), 1102–1109. doi:
  10. Schulz, E., Gori, T., Münzel, T. (2011). Oxidative stress and endothelial dysfunction in hypertension. Hypertension Research, 34 (6), 665–673. doi:
  11. Van der Zwan, L. P., Scheffer, P. G., Dekker, J. M., Stehouwer, C. D. A., Heine, R. J., Teerlink, T. (2010). Hyperglycemia and Oxidative Stress Strengthen the Association Between Myeloperoxidase and Blood Pressure. Hypertension, 55 (6), 1366–1372. doi:
  12. Lobbes, M. B. I., Kooi, M. E., Lutgens, E., Ruiters, A. W., Lima Passos, V., Braat, S. H. J. G. et. al. (2010). Leukocyte Counts, Myeloperoxidase, and Pregnancy-Associated Plasma Protein A as Biomarkers for Cardiovascular Disease: Towards a Multi-Biomarker Approach. International Journal of Vascular Medicine, 2010, 1–9. doi:
  13. Gibson, M. S., Domingues, N., Vieira, O. V. (2018). Lipid and Non-lipid Factors Affecting Macrophage Dysfunction and Inflammation in Atherosclerosis. Frontiers in Physiology, 9. doi:
  14. Karajibani, M., Hashemi, M., Montazerifar, F., Bolouri, A., Dikshit, M. (2009). The Status of Glutathione Peroxidase, Superoxide Dismutase, Vitamins A, C, E and Malondialdehyde in Patients with Cardiovascular Disease in Zahedan, Southeast Iran. Journal of Nutritional Science and Vitaminology, 55 (4), 309–316. doi:
  15. Poznyak, A. V., Grechko, A. V., Orekhova, V. A., Chegodaev, Y. S., Wu, W.-K., Orekhov, A. N. (2020). Oxidative Stress and Antioxidants in Atherosclerosis Development and Treatment. Biology, 9 (3), 60. doi:
  16. Poznyak, A. V., Nikiforov, N. G., Markin, A. M., Kashirskikh, D. A., Myasoedova, V. A., Gerasimova, E. V., Orekhov, A. N. (2021). Overview of OxLDL and Its Impact on Cardiovascular Health: Focus on Atherosclerosis. Frontiers in Pharmacology, 11. doi:
  17. Zuliani, G., Morieri, M. L., Volpato, S., Vigna, G. B., Tch, C. B., Maggio, M. et. al. (2013). Determinants and clinical significance of plasma oxidized LDLs in older individuals. A 9 years follow-up study. Atherosclerosis, 226 (1), 201–207. doi:
  18. Gómez, M., Vila, J., Elosua, R., Molina, L., Bruguera, J., Sala, J. et. al. (2014). Relationship of lipid oxidation with subclinical atherosclerosis and 10-year coronary events in general population. Atherosclerosis, 232 (1), 134–140. doi:




How to Cite

Mandsorwale, D., Sharma, B., & Singh, M. P. (2021). Role of circulating oxidized LDL: a useful diagnostic risk marker in cardiovascular disease. ScienceRise: Medical Science, (3(42), 4–8.



Medical Science