Features of age-related changes in thyroid and sex hormones levels in spontaneously hypertensive rats

Authors

DOI:

https://doi.org/10.15587/2519-4798.2025.329568

Keywords:

arterial hypertension, thyroid hormones, sex hormones, spontaneously hypertensive rats

Abstract

The course of development and progression of arterial hypertension (AH) is influenced by many factors, including the functional state of the endocrine system.

The aim of the study is to determine the content of thyrotropin, free forms of thyroid hormones, total testosterone and estradiol in the blood serum of spontaneously hypertensive rats of the SHR line of different age groups.

Materials and methods. The studies were performed on male rats aged 9 and 24 months. All animal experiments were performed in accordance with the guidelines of the EU Directive 2010/63/EU on the protection of animals used for scientific purposes. White outbred rats served as normotensive controls, and spontaneously hypertensive rats of the SHR line served as a classical model of hypertension. The study of the content of thyrotropin, free forms of thyroid hormones, total testosterone and estradiol was carried out on a microplate enzyme immunoassay analyzer "Stat Fax 3200" (Awareness Technology inc., USA) using standard commercial test kits from the company "Hema" (Ukraine).

Results. The levels of pituitary thyroid-stimulating hormone, free triiodothyronine and total testosterone were significantly higher in SHR rats of both age groups compared to age-matched controls. The level of free thyroxine remained unchanged in all groups. The level of estradiol in the serum of spontaneously hypertensive rats was significantly higher (by 60%) than that of normotensive rats.

Conclusions.  SHR rats aged 9 and 24 months are characterized by neuroendocrine features in the form of subclinical hypothyroidism, significantly increased levels of free triiodothyronine and total testosterone, which may affect the premature development of cardiovascular diseases. The level of estradiol was significantly higher only in 9-month-old SHR rats, which may explain the protective effect of these hormones on the state of the cardiovascular system of young rats with hypertension.

Author Biography

Igor Kandybko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine

PhD Student

Department of Cryophysiology

References

  1. Moroz, D. M. et al. (2016). Problemy zdorovia i medychnoi dopomohy ta model pokrashchennia v suchasnykh umovakh. Kyiv: DRUKARNIa «HORDON», 261. Available at: https://library.gov.ua/problemy-zdorovya-medychnoyi-dopomogy-ta-model-pokrashhannya-v-suchasnyh-umovah/
  2. Sirenko, Yu. M. (2014). Medical and social problems of cardiac care in Ukraine: metods of a solutions. Problemy bezperervnoi med. osvity ta nauky, 2, 6–10. Available at: http://nbuv.gov.ua/UJRN/Psmno_2014_2_3
  3. Koval, S. M., Rieznik, L. A., Starchenko, T. G. (2023). Characteristics of arterial hypertension in patients after being in a combat zone in the Kharkiv region of Ukraine. Ukrainian Therapeutical Journal, 3, 5–12. https://doi.org/10.30978/utj2023-3-5
  4. Yamakawa, H., Kato, T. S., Noh, J. Y., Yuasa, S., Kawamura, A., Fukuda, K., Aizawa, Y. (2021). Thyroid Hormone Plays an Important Role in Cardiac Function: From Bench to Bedside. Frontiers in Physiology, 12. https://doi.org/10.3389/fphys.2021.606931
  5. Kjaergaard, A. D., Marouli, E., Papadopoulou, A., Deloukas, P., Kuś, A., Sterenborg, R. et al. (2021). Thyroid function, sex hormones and sexual function: a Mendelian randomization study. European Journal of Epidemiology, 36 (3), 335–344. https://doi.org/10.1007/s10654-021-00721-z
  6. Lerman, L. O., Kurtz, T. W., Touyz, R. M., Ellison, D. H., Chade, A. R., Crowley, S. D. et al. (2019). Animal Models of Hypertension: A Scientific Statement From the American Heart Association. Hypertension, 73 (6), e87–e120. https://doi.org/10.1161/hyp.0000000000000090
  7. Bruhn, T. O., Jackson, I. M. D. (1992). Abnormalities of the thyroid hormone negative feedback regulation of TSH secretion in spontaneously hypertensive rats. Regulatory Peptides, 38 (3), 221–230. https://doi.org/10.1016/0167-0115(92)90104-3
  8. Kandybko, I. V., Babiychuk, V. G., Babiychuk, L. V., Kudokotseva, O. V., Lomakin, I. I. (2025). Age-based changes in structure and functions of the spontaneously hypertensive rats’ heart. Medicine Today and Tomorrow, 94 (1). https://doi.org/10.35339/msz.2025.94.1.kbb
  9. Weisser-Thomas, J., Nguyen, Q., Schuettel, M., Thomas, D., Dreiner, U., Grohé, C., Meyer, R. (2007). Age and hypertrophy related changes in contractile post-rest behavior and action potential properties in isolated rat myocytes. AGE, 29 (4), 205–217. https://doi.org/10.1007/s11357-007-9040-1
  10. Atramentova, L. A., Utevskaia, O. M. (2008). Statisticheskie metodi v biologii. Gorlovka: ChP «VIDAVNITcTVO LІKhTAR», 248. Available at: https://dokumen.pub/978-966-2129-26-7.html
  11. Mallya, M., Ogilvy-Stuart, A. L. (2018). Thyrotropic hormones. Best Practice & Research Clinical Endocrinology & Metabolism, 32 (1), 17–25. https://doi.org/10.1016/j.beem.2017.10.006
  12. Vavřínová, A., Behuliak, M., Vodička, M., Bencze, M., Ergang, P., Vaněčková, I., Zicha, J. (2024). More efficient adaptation of cardiovascular response to repeated restraint in spontaneously hypertensive rats: the role of autonomic nervous system. Hypertension Research, 47 (9), 2377–2392. https://doi.org/10.1038/s41440-024-01765-w
  13. Machado, N. R., Miyazaki, M. A., Oliveira, T. D., Dias, K. T., Colli, L. G., Belardin, L. B. et al. (2024). Systemic alpha-1 adrenergic receptor inhibition reduces sperm damage in adult and aging spontaneously hypertensive rats. Scientific Reports, 14 (1). https://doi.org/10.1038/s41598-024-77661-7
  14. Loh, S., Salleh, N. (2017). Influence of testosterone on mean arterial pressure: A physiological study in male and female normotensive WKY and hypertensive SHR rats. Physiology International, 104 (1), 25–34. https://doi.org/10.1556/2060.104.2017.1.3
  15. Iorga, A., Cunningham, C. M., Moazeni, S., Ruffenach, G., Umar, S., Eghbali, M. (2017). The protective role of estrogen and estrogen receptors in cardiovascular disease and the controversial use of estrogen therapy. Biology of Sex Differences, 8 (1). https://doi.org/10.1186/s13293-017-0152-8
  16. Teoh, H., Quan, A., Leung, S. W. S., Man, R. Y. K. (2000). Differential effects of 17β‐estradiol and testosterone on the contractile responses of porcine coronary arteries. British Journal of Pharmacology, 129 (7), 1301–1308. https://doi.org/10.1038/sj.bjp.0703164
  17. Reckelhoff, J. F., Zhang, H., Granger, J. P. (1998). Testosterone Exacerbates Hypertension and Reduces Pressure-Natriuresis in Male Spontaneously Hypertensive Rats. Hypertension, 31 (1), 435–439. https://doi.org/10.1161/01.hyp.31.1.435
  18. Dickey, C., Toot, J., Terwilliger, M., Payne, R., Turner, M., Ely, D. (2012). The SHR Y chromosome increases cardiovascular, endocrine, and behavioral responses to stress compared to the WKY Y chromosome. Physiology & Behavior, 106 (2), 101–108. https://doi.org/10.1016/j.physbeh.2012.01.009
  19. Stice, J. P., Lee, J. S., Pechenino, A. S., Knowlton, A. A. (2009). Estrogen, aging and the cardiovascular system. Future Cardiol., Jan, 5 (1), 93–103. https://doi.org/ 10.2217/14796678.5.1.93.
  20. Faustino, L. C., Gagnidze, K., Ortiga-Carvalho, T. M., Pfaff, D. W. (2015). Impact of Thyroid Hormones on Estrogen Receptor α-Dependent Transcriptional Mechanisms in Ventromedial Hypothalamus and Preoptic Area. Neuroendocrinology, 101 (4), 331–346. https://doi.org/10.1159/000381459
Features of age-related changes in thyroid and sex hormones levels in spontaneously hypertensive rats

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Published

2025-05-16

How to Cite

Kandybko, I. (2025). Features of age-related changes in thyroid and sex hormones levels in spontaneously hypertensive rats. ScienceRise: Medical Science, (1 (62), 20–24. https://doi.org/10.15587/2519-4798.2025.329568

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No. 1 (62) (2025)

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Medical Science

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Copyright (c) 2025 Igor Kandybko

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