Morphological changes in blood in pneumonia in children, taking into account the impact of COVID-19

Authors

DOI:

https://doi.org/10.15587/2519-4798.2025.348501

Keywords:

pneumonia, morphological changes in blood, COVID-19, hemomicrocirculatory system, SARS-CoV-2, hypoxia, lung disease

Abstract

The study of morphological changes in blood in pneumonia in children, in particular those accompanied by coronavirus infection, is an important aspect for improving diagnostics and predicting the occurrence of complications arising from the action of oxidative stress.

Objective: to identify and compare morphological changes in blood in children with pneumonia depending on the presence of COVID-19 infection, to find out which morphological indicators are associated with the severity of the disease and to assess the significance of morphological markers for the development of complications.

Materials and methods: A cohort study was conducted on the basis of the pulmonology department of the Ivano-Frankivsk Regional Children's Clinical Hospital of the Ivano-Frankivsk Regional Council in the period 2021-2025. Children aged 1-3 years with clinical and radiological confirmation of pneumonia were included. For this study, 60 young children (1-3 years old) diagnosed with community-acquired pneumonia were selected and divided into 2 groups according to the presence of complicated pneumonia.

Results: Using ultrastructural blood analysis, it was found that the most pronounced morphological changes are observed in complicated cases of COVID-19. Erythrocytes change their shape, some of them are destroyed, others participate in the formation of microthrombi. Among leukocytes, an increase in the number of segmented neutrophils, lymphocytes and monocytes is noted.

Conclusions. The results of the study will clarify the prognostic role of morphological changes in the blood in children with pneumonia, in particular with concomitant COVID-19 infection

Author Biographies

Oksana Dutchuk, Ivano-Frankivsk National Medical University

Assistant

Department of Pediatrics

Zoryana Kocherha, Ivano-Frankivsk National Medical University

Doctor of Medical Sciences, Professor, Head of Department

Department of Propaedeutics of Pediatrics

References

  1. Horiuchi, Y., Hayashi, F., Iwasaki, Y., Matsuzaki, A., Nishibe, K., Kaniyu, K. et al. (2021). Peripheral granular lymphocytopenia and dysmorphic leukocytosis as simple prognostic markers in COVID‐19. International Journal of Laboratory Hematology, 43 (6), 1309–1318. https://doi.org/10.1111/ijlh.13696
  2. Sadigh, S., Massoth, L. R., Christensen, B. B., Stefely, J. A., Keefe, J., Sohani, A. R. (2020). Peripheral blood morphologic findings in patients with COVID‐19. International Journal of Laboratory Hematology, 42 (6). https://doi.org/10.1111/ijlh.13300
  3. Wang, F., Nie, J., Wang, H., Zhao, Q., Xiong, Y., Deng, L. et al. (2020). Characteristics of Peripheral Lymphocyte Subset Alteration in COVID-19 Pneumonia. The Journal of Infectious Diseases, 221 (11), 1762–1769. https://doi.org/10.1093/infdis/jiaa150
  4. Lippi, G., Plebani, M., Henry, B. M. (2020). Thrombocytopenia is associated with severe coronavirus disease 2019 (COVID-19) infections: A meta-analysis. Clinica Chimica Acta, 506, 145–148. https://doi.org/10.1016/j.cca.2020.03.022
  5. Mehta, P., McAuley, D. F., Brown, M., Sanchez, E., Tattersall, R. S., Manson, J. J. (2020). COVID-19: consider cytokine storm syndromes and immunosuppression. The Lancet, 395 (10229), 1033–1034. https://doi.org/10.1016/s0140-6736(20)30628-0
  6. Zini, G., Bellesi, S., Ramundo, F., d’Onofrio, G. (2020). Morphological anomalies of circulating blood cells in COVID‐19. American Journal of Hematology, 95 (7), 870–872. https://doi.org/10.1002/ajh.25824
  7. Merino, A., Vlagea, A., Molina, A., Egri, N., Laguna, J., Barrera, K. et al. (2020). Atypical lymphoid cells circulating in blood in COVID-19 infection: morphology, immunophenotype and prognosis value. Journal of Clinical Pathology, 75 (2), 104–111. https://doi.org/10.1136/jclinpath-2020-207087
  8. Tan, L., Wang, Q., Zhang, D., Ding, J., Huang, Q., Tang, Y.-Q. et al. (2020). Lymphopenia predicts disease severity of COVID-19: a descriptive and predictive study. Signal Transduction and Targeted Therapy, 5 (1). https://doi.org/10.1038/s41392-020-0148-4
  9. Yamada, T., Wakabayashi, M., Yamaji, T., Chopra, N., Mikami, T., Miyashita, H., Miyashita, S. (2020). Value of leukocytosis and elevated C-reactive protein in predicting severe coronavirus 2019 (COVID-19): A systematic review and meta-analysis. Clinica Chimica Acta, 509, 235–243. https://doi.org/10.1016/j.cca.2020.06.008
  10. Tuharov, Y., Dvorshchenko, K. (2024). Oxidative modification of proteins in blood plasma of patients with osteoarthritis after SARS-CoV2 Infection. Bulletin of Taras Shevchenko National University of Kyiv. Series: Biology, 97 (2), 22–27. https://doi.org/10.17721/1728.2748.2024.97.22-27
  11. Cecchini, R., Cecchini, A. L. (2020). SARS-CoV-2 infection pathogenesis is related to oxidative stress as a response to aggression. Medical Hypotheses, 143, 110102. https://doi.org/10.1016/j.mehy.2020.110102
  12. Forcados, G. E., Muhammad, A., Oladipo, O. O., Makama, S., Meseko, C. A. (2021). Metabolic Implications of Oxidative Stress and Inflammatory Process in SARS-CoV-2 Pathogenesis: Therapeutic Potential of Natural Antioxidants. Frontiers in Cellular and Infection Microbiology, 11. https://doi.org/10.3389/fcimb.2021.654813
  13. Georgieva, E., Ananiev, J., Yovchev, Y., Arabadzhiev, G., Abrashev, H., Abrasheva, D. et al. (2023). COVID-19 Complications: Oxidative Stress, Inflammation, and Mitochondrial and Endothelial Dysfunction. International Journal of Molecular Sciences, 24 (19), 14876. https://doi.org/10.3390/ijms241914876
  14. Spiezia, L., Boscolo, A., Poletto, F., Cerruti, L., Tiberio, I., Campello, E., Navalesi, P., Simioni, P. (2020). COVID-19-Related Severe Hypercoagulability in Patients Admitted to Intensive Care Unit for Acute Respiratory Failure. Thrombosis and Haemostasis, 120 (6), 998–1000. https://doi.org/10.1055/s-0040-1710018
  15. Tang, N., Li, D., Wang, X., Sun, Z. (2020). Abnormal coagulation parameters are associated with poor prognosis in patients with novel coronavirus pneumonia. Journal of Thrombosis and Haemostasis, 18 (4), 844–847. https://doi.org/10.1111/jth.14768
  16. Harris, C. K., Hung, Y. P., Nielsen, G. P., Stone, J. R., Ferry, J. A. (2021). Bone Marrow and Peripheral Blood Findings in Patients Infected by SARS-CoV-2. American Journal of Clinical Pathology, 155 (5), 627–637. https://doi.org/10.1093/ajcp/aqaa274
  17. Bahadur, S., Kalonia, T., Kamini, K., Gupta, B., Kalhan, S., Jain, M. (2021). Changes in peripheral blood in SARS CoV‐2 patients and its clinico‐pathological correlation: A prospective cross‐sectional study. International Journal of Laboratory Hematology, 43 (6), 1334–1340. https://doi.org/10.1111/ijlh.13720
  18. Cattaneo, M., Bertinato, E. M., Birocchi, S., Brizio, C., Malavolta, D., Manzoni, M. et al. (2020). Pulmonary Embolism or Pulmonary Thrombosis in COVID-19? Is the Recommendation to Use High-Dose Heparin for Thromboprophylaxis Justified? Thrombosis and Haemostasis, 120 (8), 1230–1232. https://doi.org/10.1055/s-0040-1712097
  19. Grobbelaar, L. M., Venter, C., Vlok, M., Ngoepe, M., Laubscher, G. J., Lourens, P. J. et al. (2021). SARS-CoV-2 spike protein S1 induces fibrin(ogen) resistant to fibrinolysis: implications for microclot formation in COVID-19. Bioscience Reports, 41 (8). https://doi.org/10.1042/bsr20210611
  20. Matharu, S. S., Nordmann, C. S., Ottman, K. R., Akkem, R., Palumbo, D., Cruz, D. R. D. et al. (2023). Deep learning, 3D ultrastructural analysis reveals quantitative differences in platelet and organelle packing in COVID-19/SARSCoV2 patient-derived platelets. Platelets, 34 (1). https://doi.org/10.1080/09537104.2023.2264978
Morphological changes in blood in pneumonia in children, taking into account the impact of COVID-19

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Published

2025-11-30

How to Cite

Dutchuk, O., & Kocherha, Z. (2025). Morphological changes in blood in pneumonia in children, taking into account the impact of COVID-19. ScienceRise: Medical Science, (3 (64), 54–60. https://doi.org/10.15587/2519-4798.2025.348501

Issue

No. 3 (64) (2025)

Section

Medical Science

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Copyright (c) 2025 Oksana Dutchuk, Zoryana Kocherha

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