Phenotypic features of generated dendritic cells in patients with pancreatic cancer immunotherapy
DOI:
https://doi.org/10.15587/2519-4798.2017.90950Keywords:
pancreatic cancer, immunotherapy, anticancer vaccines, dendritic cells, phenotypic featuresAbstract
Aim. The aim of our research was to study quantitative and phenotypic features of dendritic cells (DC) from peripheral blood monocytes for immunotherapy in patients with pancreatic cancer (PC).
Materials and methods. DC were used as natural adjuvants in the composition of anticancer vaccines in complex treatment of PC patients. DC phenotypic maturity was estimated using flow cytofluorometry.
Results and discussion. The analysis of DC quantity and vitality has shown that in all PC patients it managed to grow a sufficient number of viable DC for immunotherapy. The expression level of CD83, CD86 и HLA-DR differentiation antigens on DC generated in vitro significantly increased during immunotherapy in PC patients.
Conclusion. The obtained data can be put into the basis for development of the protocols of DC use as natural adjuvants in the composition of anticancer vaccines in the standard schemes of PC patients’ treatmentReferences
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